UCHL1 / PGP9.5 antibody

Principal name

UCHL1 / PGP9.5 antibody

Alternative names for UCHL1 / PGP9.5 antibody

UCH-L1, PGP 9.5, Ubiquitin thioesterase L1, Neuron cytoplasmic protein 9.5, Ubiquitin carboxyl-terminal hydrolase isozyme L1

SwissProt ID

P09936 (Human), P23356 (Bovin), Q00981 (Rat), Q6SEG5 (Pig), Q9GM50 (Horse), Q9R0P9 (Mouse)

Gene ID

7345 (UCHL1), 4747 (NEFL), 7038 (TG), 22223 (Uchl1)

Available reactivities

Hu (Human), Bov (Bovine), GP (Guinea Pig), Ms (Mouse), Por (Porcine), Rt (Rat), Eq (Equine), Mky (Monkey), Can (Canine), Fe (Feline), Hst (Hamster), Rb (Rabbit), Sh (Sheep), Mam (Mammalia), Prim (Primate), Ze (Zebrafish)

Available hosts

Goat, Guinea Pig, Mouse, Rabbit, Chicken

Available applications

Western blot / Immunoblot (WB), Frozen Sections (C), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Dot blot (Dot), Flow Cytometry (F)

Background of UCHL1 / PGP9.5 antibody

Ubiquitin C-terminal hydrolase 1 (UCHL1) was originally identified as a major component of the neuronal cytoplasm from 2-dimensional gel analysis of brain tissues, and was given the name PGP9.5. The protein is extremely abundant representing 1-2% of total brain protein. It was later found that a ubiquitin C-terminal hydrolase enzyme activity was associated with the PGP9.5 protein, resulting in the renaming of PGP9.5 to ubiquitin C-terminal hydrolase 1. This is the first of a family of ubiquitin C-terminal hydrolases which have been characterized, and is expressed heavily in neurons in the brain. The ubiquitin C-terminal hydrolases cleave ubiquitin from other molecules. This activity is important to generate mono-ubiquitin from genes which encode polyubiquitin chains or ubiquitin fused to other proteins. The activity is also important to remove ubiquitin from partially degraded proteins, allowing the ubiquitin monomer to be recycled. Regulation of the ubiquitin pathway is very important and many disease states are associated with defects in this pathway. Point mutations in the UCHL1 gene are associated with some forms of human Parkinson's disease. Recent studies suggest that UCHL1 also has a ubiqutinyl ligase activity, being able to couple ubiquitin monomers by linking the C-terminus of one with lysine 63 of the other. Since UCHL1 is heavily expressed in neurons, antibodies to UCHL1 can be used to identify neurons in histological sections and in tissue culture. The great abundance of this protein in neurons means that it is released from neurons in large amounts following injury or degeneration, so the detection of of UCHL1 in CSF and other bodily fluids can be used as a biomarker. UCHL1 was also discovered as a gene mutated in some rare famial forms of Parkinson's disease. Interestingly a common allelic variant of UCHL1, the S18Y polymorphism is actually protective against Parkinson's disease.

General readings

1. Xue, L. et al. (2000) Carbon monoxide and nitric oxide coneurotransmitters in the enteric nervous system: Evidence from genomic deletion of biosynthetic enzymes. P.N.A.S. 97: 1851-1855.
2. Adolfsson, P.I. et al. (2000) Changes in beta2-adrenoreceptor expression and in adenylyl cyclase and phosphodiesterase activity in human uterine leiomyomas. Mol. Hum. Repro. 6: 835-842.
3. Hibi, K. et al. (1999) PGP9.5 as a candidate tumor marker for non-small-cell lung cancer.
Am. J. Pathol. 155: 711-715.
4. Yamazaki, T. et al. (2002) PGP9.5 as a marker for invasive colorectal cancer. Clin. Cancer Res. 8: 192-195.
5. Donadio, V. et al. (2008) Isolated generalised anhidrosis induced by postganglionic sympathetic skin nerve fibre degeneration: an incomplete Ross syndrome? J Neurol Neurosurg Psychiatry. 79: 959-961.
6. De Filippis, D. et al. (2011) Palmitoylthanolamide reduces granuloma-induced hyperalgesia by modulation of mast cell activation in rats. Mol Pain. 7: 3.
7. Hamed, K. et al. (2011) Changes in cutaneous innervation in patients with chronic pain after burns. Burns. Jan 21. [Epub ahead of print]
8. Almond, S. et al. (2007) Characterisation and transplantation of enteric nervous system progenitor cells. Gut. 56: 489-96.
9. Lin, W. et al.,/i> (2008) TRPM5-expressing microvillous cells in the main olfactory epithelium. BMC Neurosci. 9:114.
10. Shneider, N.A. et al. (2009) Gamma motor neurons express distinct genetic markers at birth and require muscle spindle-derived GDNF for postnatal survival. Neural Dev. 4: 42.
11. Chang, J. et al. (2011) Urocortin 1 modulates immunosignaling in a rat model of colitis via corticotropin-releasing factor receptor 2.
Am J Physiol Gastrointest Liver Physiol. 300: G884-94.
12. Williams, L. et al. (2007) The effect of faecal diversion on human ileum. Gut. 56: 796-801.
12. Saporta, M.A. et al. (2011) Neuropathy in a Human Without the PMP22 Gene. Arch Neurol. 68: 814-21.
13. Arms, L. and Vizzard, M.A. (2011) Role for pAKT in Rat Urinary Bladder with Cyclophosphamide (CYP)- Induced Cystitis. Am J Physiol Renal Physiol. Jun 1. [Epub ahead of print]
14. Liao, Z. and Smith, P.G. (2011) Adaptive plasticity of vaginal innervation in term pregnant rats. Reprod Sci. 18: 1237-45.
15. Day, I.N.M. & Thompson, R. (2010) UCHL1 (PGP 9.5): Neuronal biomarker and ubiquitin system protein. Prog. Neurobiol. 90: 327-362.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

UCHL1 / PGP9.5 antibody

Western blot of rat hippocampal homogenate showing specific immunolabeling of the ~ 24k UCHL1 protein. Chicken Ig Ig Fraction Bov, Hu, Mam, Ms, Rt ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

UCHL1 / PGP9.5 (211-221) antibody

AP21292PU-N UCHL1 antibody staining of Mouse (A) and Rat (B) Brain lysate at 0.01 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Por, Rt E, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

UCHL1 / PGP9.5 (C-term) antibody

UCHL1 Antibody (C-term) (Cat.# TA324710) western blot analysis in U266,NCI-H1299,mouse Neuro-2a cell line and mouse brain tissue lysates (35ug/lane).This demonstrates the UCHL1 antibody detected the UCHL1 protein (arrow). Rabbit Hu, Ms WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

UCHL1 / PGP9.5 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-UCHL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504289) Mouse IgG1 OTI2B5 Purified Hu, Mky, Rt F, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

UCHL1 / PGP9.5 (N-term) antibody

Formalin-fixed and paraffin-embedded human brain tissue reacted with UCHL1 (Park5) antibody (N-term) (Cat.-No AP11992PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu, Ms P, WB

Regular Price: 0.4 ml / €370.00

Special Price: 0.4 ml / €231.00

  Acris Antibodies GmbH

UCHL1 / PGP9.5 antibody

Formalin-Fixed, Paraffin-Embedded Human brain stained with UCHL1 / PGP9.5 Antibody Cat.-No AM50184PU (Clone SPM574). Mouse IgG1 SPM574 Purified Bov, Hu, Ms, Por, Rt P, WB
0.2 mg / €390.00
  Acris Antibodies GmbH

UCHL1 / PGP9.5 antibody

Formalin-Fixed, Paraffin-Embedded Human brain stained with UCHL1 / PGP9.5 Antibody Cat.-No AM50184PU (Clone SPM574). Mouse IgG1 SPM574 Purified Bov, Hu, Ms, Por, Rt P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

UCHL1 / PGP9.5 antibody

Formalin-Fixed, Paraffin-Embedded Human brain stained with UCHL1 / PGP9.5 Antibody Cat.-No AM50184PU (Clone SPM574). Mouse IgG1 SPM574 Purified Bov, Hu, Ms, Por, Rt P, WB
20 µg / €160.00
  Acris Antibodies GmbH

UCHL1 / PGP9.5 antibody

AP15750PU-N PGP9.5 antibody staining of Human pancreas Rabbit Purified Hu P, WB
0.5 ml / €360.00
  Acris Antibodies GmbH

UCHL1 / PGP9.5 antibody

AP15750PU-N PGP9.5 antibody staining of Human pancreas Rabbit Purified Hu P, WB
1 ml / €440.00
  Acris Antibodies GmbH

UCHL1 / PGP9.5 antibody

AP15750PU-N PGP9.5 antibody staining of Human pancreas Rabbit Purified Hu P, WB
0.1 ml / €180.00
  Acris Antibodies GmbH

UCHL1 / PGP9.5 (58-68) antibody

AP21291PU-N UCHL1 antibody staining of Mouse (A) and Rat (B) Brain lysates at 0.01 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Por, Rt E, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

UCHL1 / PGP9.5 antibody

Formalin-Fixed, Paraffin-Embedded Human brain stained with UCHL1 / PGP9.5 Antibody Cat.-No BM699 (Clone 31A3). Mouse IgG1 31A3 Purified Bov, Hu, Ms, Por, Rt F, P, WB
0.2 mg / €390.00
  Acris Antibodies GmbH

UCHL1 / PGP9.5 antibody

Formalin-Fixed, Paraffin-Embedded Human brain stained with UCHL1 / PGP9.5 Antibody Cat.-No BM699 (Clone 31A3). Mouse IgG1 31A3 Purified Bov, Hu, Ms, Por, Rt F, P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

UCHL1 / PGP9.5 antibody

Formalin-Fixed, Paraffin-Embedded Human brain stained with UCHL1 / PGP9.5 Antibody Cat.-No BM699 (Clone 31A3). Mouse IgG1 31A3 Purified Bov, Hu, Ms, Por, Rt F, P, WB
20 µg / €160.00
  Acris Antibodies GmbH

UCHL1 / PGP9.5 antibody

Detection of UCHL1 in mouse brain lysate. ECL detection, 60 seconds. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

UCHL1 / PGP9.5 antibody

Detection of UCHL1 in mouse brain lysate using NB 300-676. ECL detection, 60 seconds Rabbit IgG Purified Eq, Hu, Mky, Ms, Por, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

UCHL1 / PGP9.5 antibody

Western blot of rat hippocampal homogenate showing specific immunolabeling of the ~ 24k UCHL1 protein. Mouse IgG1 BH7 Supernatant Bov, Hu, Mam, Ms, Rt C, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

UCHL1 / PGP9.5 antibody

Western blot analysis of extracts from 293 cells, using PGP9.5 antibody (#TA312138).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

UCHL1 / PGP9.5 antibody

Predicted band size: 25 kDa . Positive control: A549 and Y79 cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: A549 cell lysate Lane2: Y79 cell lysate Lysates: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

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