UCHL1 / PGP9.5 antibody

Principal name

UCHL1 / PGP9.5 antibody

Alternative names for UCHL1 / PGP9.5 antibody

UCH-L1, PGP 9.5, Ubiquitin thioesterase L1, Neuron cytoplasmic protein 9.5, Ubiquitin carboxyl-terminal hydrolase isozyme L1

SwissProt ID

P09936 (Human), P23356 (Bovin), Q00981 (Rat), Q6SEG5 (Pig), Q9GM50 (Horse), Q9R0P9 (Mouse)

Gene ID

7345 (UCHL1), 22223 (Uchl1), 29545

Available reactivities

Hu (Human), Rt (Rat), Ms (Mouse), Bov (Bovine), Can (Canine), Por (Porcine), GP (Guinea Pig), Prim (Primate), Rb (Rabbit), Sh (Sheep), Ze (Zebrafish), Mky (Monkey), Eq (Equine), Mam (Mammalia)

Available hosts

Chicken, Rabbit, Goat, Guinea Pig, Mouse

Available applications

Paraffin Sections (P), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Frozen Sections (C), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Immunoprecipitation (IP)

Background of UCHL1 / PGP9.5 antibody

Protein ubiquitition and deubiquitition are reversible processes catalyzed by ubiquititing enzymes (UBEs) and deubiquititing enzymes (DUBs). DUBs are categorized into 5 subfamilies: USP, UCH, OTU, MJD, and JAMM. UCHL1, UCHL3, UCHL5/UCH37, and BRCA-1-associated protein-1 (BAP1) belong to the UCH family of DUBs, which all posses a conserved catalytic domain (UCH domain) of about 230 amino acids. UCHL5 and BAP1 have unique extended C-termil tails. UCHL1 is abundantly expressed in neurol tissues and testes, while UCHL3 expression is more widely distributed. Although UCHL1 and UCHL3 are the most closely related UCH family members with about 53% identity, their biochemical properties differ in that UCHL1 binds monoubiquitin and UCHL3 shows dual specificity toward both ubiquitin (Ub) and NEDD8, a Ub-like molecule. In particular, UCHL3 functions as a Ub hydrolase involved in the processing of both Ub precursors and ubiquitited substrates, generating free monomeric Ub. This is accomplished through the ability of UCHL3 to recognize and hydrolyze isopeptide bonds at the C-termil glycine of either Ub or NEDD8. Recent functiol studies have identified UCH-L3 as a critical regulator of adipogenesis through its ability to promote IGF-IR and insulin receptor sigling. Furthermore, UCHL3 has been shown to promote deubiquitition, recycling, and cell surface expression of the epithelial sodium channel.

General readings

Liu Y et al , (2002) Cell 111(2):209-18. 
Mikako Sakurai.et al , (2006) Cell 119:162-171. 
Kenji H et al , (1999) Am J Pathol 155(3):711-715.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

UCHL1 / PGP9.5 antibody

  Chicken Anti-Ubiquitin C-terminal Hydrolase 1 (UCHL1) ck Antibody  
Western blot of rat hippocampal homogenate showing specific immunolabeling of the ~ 24k UCHL1 protein. Chicken Ig Ig Fraction Bov, Hu, Mam, Ms, Rt ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

UCHL1 / PGP9.5 (211-221) antibody

AP21292PU-N UCHL1 antibody staining of Mouse (A) and Rat (B) Brain lysate at 0.01 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Por, Rt E, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

UCHL1 / PGP9.5 (C-term) antibody

  Rabbit polyclonal UCHL1 Antibody (C-term)  
UCHL1 Antibody (C-term) (Cat.# TA324710) western blot analysis in U266,NCI-H1299,mouse Neuro-2a cell line and mouse brain tissue lysates (35ug/lane).This demonstrates the UCHL1 antibody detected the UCHL1 protein (arrow). Rabbit Hu, Ms WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

UCHL1 / PGP9.5 antibody

  UCHL1 (PGP9.5) mouse monoclonal antibody, clone OTI2B5 (formerly 2B5)  
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-UCHL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504289) Mouse IgG1 OTI2B5 Purified Hu, Mky, Rt F, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

UCHL1 / PGP9.5 (N-term) antibody

Formalin-fixed and paraffin-embedded human brain tissue reacted with UCHL1 (Park5) antibody (N-term) (Cat.-No AP11992PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

UCHL1 / PGP9.5 antibody

Formalin-Fixed, Paraffin-Embedded Human brain stained with UCHL1 / PGP9.5 Antibody Cat.-No AM50184PU (Clone SPM574). Mouse IgG1 SPM574 Purified Bov, Hu, Ms, Por, Rt P, WB
0.2 mg / €410.00
  Acris Antibodies GmbH

UCHL1 / PGP9.5 antibody

Formalin-Fixed, Paraffin-Embedded Human brain stained with UCHL1 / PGP9.5 Antibody Cat.-No AM50184PU (Clone SPM574). Mouse IgG1 SPM574 Purified Bov, Hu, Ms, Por, Rt P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

UCHL1 / PGP9.5 antibody

Formalin-Fixed, Paraffin-Embedded Human brain stained with UCHL1 / PGP9.5 Antibody Cat.-No AM50184PU (Clone SPM574). Mouse IgG1 SPM574 Purified Bov, Hu, Ms, Por, Rt P, WB
20 µg / €180.00
  Acris Antibodies GmbH

UCHL1 / PGP9.5 (58-68) antibody

AP21291PU-N UCHL1 antibody staining of Mouse (A) and Rat (B) Brain lysates at 0.01 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Por, Rt E, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

UCHL1 / PGP9.5 antibody

  Rabbit Polyclonal Antibody against PGP9.5 - Peripheral Nerve Fiber marker  
Detection of UCHL1 in mouse brain lysate. ECL detection, 60 seconds. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

UCHL1 / PGP9.5 antibody

  Rabbit Polyclonal Antibody against UCHL1 (PGP9.5) - Neuronal Marker - Neuronal Marker  
Detection of UCHL1 in mouse brain lysate using NB 300-676. ECL detection, 60 seconds Rabbit IgG Purified Eq, Hu, Mky, Ms, Por, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

UCHL1 / PGP9.5 antibody

  Mouse Anti-Ubiquitin C-terminal Hydrolase 1 (UCHL1) ms Antibody  
Western blot of rat hippocampal homogenate showing specific immunolabeling of the ~ 24k UCHL1 protein. Mouse IgG1 BH7 Supernatant Bov, Hu, Mam, Ms, Rt C, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

UCHL1 / PGP9.5 antibody

  Rabbit polyclonal anti-PGP9.5 antibody  
Western blot analysis of extracts from 293 cells, using PGP9.5 antibody (#TA312138).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

UCHL1 / PGP9.5 antibody

  Anti-UCHL1 Rabbit Polyclonal Antibody  
Predicted band size: 25 kDa . Positive control: A549 and Y79 cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: A549 cell lysate Lane2: Y79 cell lysate Lysates: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

UCHL1 / PGP9.5 antibody

  Mouse monoclonal UCHL1 Antibody (C-term)  
UCHL1 Antibody (C-term)(Cat. #TA324990) western blot analysis in 293T,NCI-H1299,rat PC-12 cell line lysates (35ug/lane).This demonstrates the UCHL1 antibody detected the UCHL1 protein (arrow). Mouse IgG1 696CT2.1.4 Purified Bov, Hu, Mky, Ms, Por, Rt WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

UCHL1 / PGP9.5 antibody

  Rabbit anti-UCHL1 Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using UCHL1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

UCHL1 / PGP9.5 antibody

  Rabbit Polyclonal PGP 9.5 Antibody  
Immunohistochemistry staining of Paraffin Nerve tissue tissue by PGP 9.5 antibody (dilution: 1:100 - 1:500; visualization of staining: Cytoplasmic) Rabbit Purified Hu P
1 ml / €800.00
  OriGene Technologies, Inc.

UCHL1 / PGP9.5 antibody

  Mouse Monoclonal PGP9.5 / UCHL-1 Antibody (BH7)  
Western Blot: PGP9.5 / UCHL-1 Antibody (BH7) [TA336530] - Blots of whole cell homogenate of bovine brain stained with TA336530 (right most lane) and two other monoclonal antibodies reactive with UCHL1 (left and central lane). All three antibodies show a s Mouse IgG1 BH7 Purified Bov, Hu, Rt ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

UCHL1 / PGP9.5 antibody

  Chicken Polyclonal PGP9.5 / UCHL-1 Antibody  
Western Blot: PGP9.5 / UCHL-1 Antibody [TA336532] -  Blots of whole cell homogenate of the human SH-SY5Y neuroblastoma cell line stained with chicken antibody to glyceraldehyde 3 phosphate dehydrogenase (GAPDH, left lane, blot made with monoclonal antibod Chicken IgY Purified Hu, Rt ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

UCHL1 / PGP9.5 antibody

  Rabbit Polyclonal PGP9.5 / UCHL-1 Antibody pan-  
Western Blot: PGP9.5 / UCHL-1 Antibody [TA336533] - Blot of whole bovine brain extract stained with RPCA-UCHL1 showing a strong and clean band at about 24kDa. Rabbit Purified Bov, Hu, Ms, Por, Rt ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

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