UCHL3 antibody

Principal name

UCHL3 antibody

Alternative names for UCHL3 antibody

UCH-L3, Ubiquitin thioesterase L3, Ubiquitin carboxyl-terminal hydrolase isozyme L3

SwissProt ID

P15374 (Human), Q06AB3 (Pig), Q2TBG8 (Bovin), Q91Y78 (Rat), Q9JKB1 (Mouse)

Gene ID

7347 (UCHL3)

Available reactivities

Hu (Human), Rt (Rat), Ms (Mouse), Bov (Bovine), Can (Canine), Chk (Chicken), Por (Porcine), Ze (Zebrafish), African clawed frog, Rb (Rabbit), Eq (Equine), Mky (Monkey), GP (Guinea Pig), Ye (Yeast)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of UCHL3 antibody

Deubiquititing enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitited proteins. Thiol protease that recognizes and hydrolyzes a peptide bond at the C-termil glycine of either ubiquitin or NEDD8. Uchl3 has a 10-fold preference for Arg and Lys at position P3', and exhibits a preference towards 'Lys-48'-linked Ubiquitin chains. Uchl3 deubiquitites EC in apical compartments, thereby regulating apical membrane recycling. Uchl3 indirectly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin-sigling and insulin-induced adipogenesis. It is required for stress-response retil, skeletal muscle and germ cell maintence. Uchl3 may be involved in working memory and can hydrolyze UBB(+1), a mutated form of ubiquitin which is not effectively degraded by the proteasome.

General readings

Zhang Y., et al. (2000) J. Mol. Endocrinol. 25:221-228.
Zhang Y., et al. (2009) Mol Cell Endicrinol. 298(1-2):76-83.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

UCHL3 antibody

Anti-UCHL3 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Purified Hu
50 µg / €379.00
  OriGene Technologies, Inc.

UCHL3 antibody

Anti-UCHL3 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Aff - Purified Hu, Ms, Rt P
50 µg / €379.00
  OriGene Technologies, Inc.

UCHL3 antibody

Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu, Ms, Rt P
50 µg / €379.00
  OriGene Technologies, Inc.

UCHL3 antibody

WB Suggested Anti-UCHL3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human Liver Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

UCHL3 antibody

Host: Rabbit; Target Name: Uchl3; Sample Tissue: Mouse Testis lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ye WB
50 µg / €325.00
  OriGene Technologies, Inc.

UCHL3 antibody

WB Suggested Anti-UCHL3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: MCF7 cell lysate Rabbit IgG Purified Bov, Can, GP, Hu, Ms, Por, Rb, Rt, Ze P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

UCHL3 antibody

UCHL3 polyclonal antibody (A01). Western Blot analysis of UCHL3 expression in human ovary cancer. Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

UCHL3 antibody

UCHL3 monoclonal antibody (M01), clone 4E9. Western Blot analysis of UCHL3 expression in PC-12 ( Cat # L012V1 ). Mouse IgG2a 4E9 Purified Hu, Rt E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

UCHL3 antibody

Immunoblot analysis of cell and tissue lysates.
Lane 1 : U87 MG cell lysate.
 Lnae 2 : SK-N-MC cell lysate.
Lane 3 : SK-N-SH cell lysate.
Lane 4 : IMR-32 cell lysate.
Lane 5 : PC-12 cell lysate.
Lane 6 : Mouse brain tissue lysate. Mouse AF9G8 Purified Hu, Ms, Rt E, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

UCHL3 antibody

Human Placenta; Placenta; UCHL3 antibody - N-terminal region (ARP58689_P050) in Human Placenta cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Por, Rt, Ze P, WB
50 µg / €350.00
  AVIVA Systems Biology

UCHL3 (C-term) antibody

UCHL3 Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

UCHL3 antibody

Western Blot: UCHL3 Antibody [NBP1-49711] - Detection of Human and Mouse UCH-L3 by Western Blot (h&m) and Immunoprecipitation (h).  Samples:  Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells.  Antibodies: Affinity purified rabbit anti-UCH-L3 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate.  UCH-L3 was also immunoprecipitated by rabbit anti-UCH-L3 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B). Rabbit Aff - Purified Hu, Ms IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

UCHL3 antibody

Western Blot: UCHL3 Antibody [NBP1-55316] - This Anti-UCHL3 antibody was used in Western Blot of MCF7     tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu, Ms, Rt P, WB
50 µg / €440.00
  Novus Biologicals Inc.

UCHL3 antibody

Immunohistochemistry-Paraffin: UCHL3 Antibody [NBP1-86037] - Immunohistochemical staining of human testis shows cytoplasmic positivity in cells of seminiferus ducts. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

UCHL3

UCHL3 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

UCHL3

UCHL3 Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
10 µg / €399.00
  OriGene Technologies, Inc.

UCHL3 (1-230, His-tag)

UCHL3 Human Purified > 95 % E. coli
0.5 mg / €750.00
  Acris Antibodies GmbH

UCHL3 (1-230, His-tag)

UCHL3 Human Purified > 95 % E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

UCHL3

UCHL3 Human in vitro transl.
2 µg / €300.00
  Abnova Taiwan Corp.

UCHL3

UCHL3 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

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