UCHL5 antibody

Principal name

UCHL5 antibody

Alternative names for UCHL5 antibody

UCH-L5, Ubiquitin thioesterase L5, UCH37, AD-019, CGI-70, Ubiquitin carboxyl-terminal hydrolase isozyme L5, Ubiquitin C-terminal hydrolase UCH37

SwissProt ID

Q06AT3 (Pig), Q9WUP7 (Mouse), Q9XSJ0 (Bovin), Q9Y5K5 (Human)

Gene ID

51377 (UCHL5)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Rabbit, Mouse

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Immunoprecipitation (IP)

Background of UCHL5 antibody

Covalent attachment of the C-terminus of ubiquitin to cellular proteins plays a role in a variety of cellular processes. Ubiquitin C-terminal hydrolysis is catalyzed by deubiquitinating (DUB) enzymes and is necessary for several functions, including liberation of monomeric ubiquitin from the precursors encoded by ubiquitin genes and recycling of ubiquitin monomers. There are 2 distinct families of DUBs, ubiquitin-specific proteases (UBPs) and ubiquitin C-terminal hydrolases (UCHs). Mayer and Wilkinson (1989) identified 4 distinct UCH activities from bovine thymus. All 4 were thiol proteases and had high-affinity binding sites for ubiquitin. Wilkinson et al. (1989) purified the predominant isozyme, UCHL3, and raised antibodies against it. By screening a human B-cell expression library with the antibodies, the authors isolated cDNAs encoding human UCHL3. Sequence comparisons revealed that the sequence of the predicted 230-amino acid human UCHL3 protein is 54% identical to that of UCHL1.

General readings

Yao T., et al. (2006) Nat. Cell Biol. 8:994-1002
Nishio K, et al.(2009) Biochem Biophys Res Commun. 390(3):855-60.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

UCHL5 antibody

UCH37(UCHL5) Antibody(Cat. #TA324998) western blot analysis in 293,Hela cell line lysates (35ug/lane).This demonstrates the UCH37 (UCHL5) antibody detected the UCH37 (UCHL5) protein (arrow). Mouse IgG1 854CT5.5.6 Ascites Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

UCHL5 antibody

UCH37 (UCHL5) Antibody (N-term) (Cat. #TA325168) western blot analysis in 293,Hela,293T cell line lysates (35ug/lane).This demonstrates the UCHL5 antibody detected the UCHL5 protein (arrow). Mouse IgG1 854CT5.5.6 Purified Hu WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

UCHL5 antibody

WB Suggested Anti-UCHL5 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

UCHL5 antibody

WB Suggested Anti-UCHL5 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

UCHL5 antibody

WB Suggested Anti-UCHL5 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Human Small Intestine Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

UCHL5 (N-term) antibody

HE staining of frozen human ovarian cancer tissue reacted with the primary antibody at a 1:250 dilution. Levels using the antibody on frozen tissue array (A )correlated well with the mRNA expression levels detected by Agilent expression microarray (B). This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. 60X magnification. Data courtesy of Marlena Fejzo, University of California, Los Angeles. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

UCHL5 antibody

UCHL5 MaxPab polyclonal antibody. Western Blot analysis of UCHL5 expression in human placenta. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

UCHL5 antibody

HE staining of frozen human ovarian cancer tissue reacted with UCHL5 polyclonal antibody ( Cat # PAB3907 ) at a 1 : 250 dilution. Levels using the antibody on frozen tissue array ( A ) correlated well with the mRNA expression levels detected by Agilent expression microarray ( B ) . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. 60X magnification. Data courtesy of Marlena Fejzo, University of California, Los Angeles. Rabbit Hu, Ms C, E, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

UCHL5 antibody

Zebrafish skin; WB Suggested Anti-UCHL5 Antibody . . Positive Control: Lane 1: 30ug Zebrafish skin lysate . Primary Antibody Dilution : 1:1000. Secondary Antibody : Anti-rabbit-HRP . Secondry Antibody Dilution : 1:4000. Submitted by: William Tse; UCHL5 antibody - middle region (ARP56797_P050) in Zebrafish skin cells using Western Blot Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

UCHL5 (C-term) antibody

UCHL5 Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

UCHL5 antibody

UCHL5 Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

UCHL5 antibody

Immunohistochemistry-Paraffin: UCH37 Antibody [NBP1-85656] - Immunohistochemical staining of human cerebral cortex shows strong nuclear(nucleolar) positivity in neuronal cells. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

UCHL5 antibody

Immunohistochemistry-Paraffin: UCH37 Antibody (EPR4896) [NBP1-95618] human kidney tissue using  anti-UCH37. Rabbit EPR4896 Purified Hu, Rt F, IP, P, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

UCHL5 antibody

Immunohistochemistry-Paraffin: UCH37 Antibody (EP4897) [NBP1-95831] human kidney tissue using anti-UCHL5 / UCH37. Rabbit EP4897 Purified Hu, Ms, Rt ICC/IF, IP, P, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

UCHL5

UCHL5 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

UCHL5 (1-329, His-tag)

UCHL5 Human Purified > 90 % E. coli
0.5 mg / €750.00
  Acris Antibodies GmbH

UCHL5 (1-329, His-tag)

UCHL5 Human Purified > 90 % E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

UCHL5

UCHL5 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

UCHL5

UCHL5 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

UCHL5

UCHL5 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

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