UFD1L antibody

Principal name

UFD1L antibody

Alternative names for UFD1L antibody

UB fusion protein 1, Ubiquitin fusion degradation protein 1 homolog

SwissProt ID

P70362 (Mouse), Q92890 (Human)

Gene ID

7353 (UFD1L)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of UFD1L antibody

The U box is a domain of approximately 70 amino acids that is present in proteins from yeast to humans. The prototype U box protein, yeast Ufd2, was identified as a ubiquitin chain assembly factor that cooperates with a ubiquitin-activating enzymes (E1, E2), and a ubiquitin-protein ligase (E3) to catalyze ubiquitin chain formation on artificial substrates. UFD1L is an essential component of the ubiquitin-dependent proteolytic pathway which degrades ubiquitin fusion proteins. The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteosome. In mammalian cells the membrane bound ubiquitin ligase gp75 promotes degradation of sterol regulated degradation of HMG CoA reductase and misfolded endoplasmic reticulum proteins. The ubiquitin fusion degradation 1 like (UFD1L) protein plays a critical role in ER associated degradation in association with Npl4 and VCP proteins. Interaction of VCP-UFD1L-Npl4 complex recognizes polyubiquitin chains and transfers the ubiquitinated proteins to proteosomes. The UFD1L also directly interacts with membrane bound gp75 as a cofactor and accelerate the ubiquitination and degradation of reductase and eventually promotes receptor mediated uptake of low density lipoproteins, thus plays an important role in cholesterol metabolism (1). It is shown that UFD1L-Npl4 complex is a negative regulator of retrotranslocation of A1 chain of the cholera toxin (CT) to the cytosol across the ER, delaying the retro-translocation of ERAD substrates independently of its association with VCP (2). The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. It may be involved in the development of some ectoderm-derived structures.
UFD1L gene is located on chromosome22q1.2 and is expressed during embryogenesis in the eyes and in the linear ear primordial. UFD1L gene is expressed in patients with DiGeorge (DGS) and velo-cardial facial (VCF) syndrome which suggest that proteolytic pathways that recognize ubiquitin fusion proteins for degrradtioin is conserved in vertebrates and UFD1L gene hemizygosity is the cause of some of the CATCH-22 (acronym for cardiac defects, abnormal facies, thymic hypoplasia, cleft palate and hypocalcimea) associated developmental defects in DGS and VCSF syndromes (3). UFD1L is expressed in adult heart, skeletal muscle and pancreas, and in fetal liver and kidney. UFD1L is a 307 amino acid protein apparent MW is 38kDa.

General readings

1. Cao J, Wang J, Qi W, Miao HH, Wang J, Ge L, DeBose-Boyd RA, Tang JJ, Li BL, Song BL. Cell Metab. 2007 Aug;6(2):115-28.
2. .McConnell E, Lass A, Wójcik C. Biochem Biophys Res Commun. 2007 Apr 20;355(4):1087-90.
3. Pizzuti A, Novelli G, Ratti A, Amati F, Mari A, Calabrese G, Nicolis S, Silani V, Marino B, Scarlato G, Ottolenghi S, Dallapiccola B. Hum Mol
Genet. 1997 Feb;6(2):259-65.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

UFD1L antibody

  Rabbit polyclonal antibody to UFD1L (ubiquitin fusion degradation 1 like (yeast))  
Sample (30 ug of whole cell lysate). A: A431 . B: H1299. 10% SDS PAGETA308249 diluted at 1:10000 Rabbit IgG Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

UFD1L antibody

  UFD1L mouse monoclonal antibody, clone OTI8G6 (formerly 8G6)  
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-UFD1L mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804317) Mouse IgG1 OTI8G6 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

UFD1L antibody

  UFD1L mouse monoclonal antibody, clone OTI7C9 (formerly 7C9)  
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-UFD1L mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804319) Mouse IgG2b OTI7C9 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

UFD1L antibody

  Rabbit Polyclonal Anti-UFD1L Antibody  
WB Suggested Anti-UFD1L Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: MCF7 cell lysate UFD1L is supported by BioGPS gene expression data to be expressed in MCF7 Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

UFD1L antibody

  Purified UFD1L mouse monoclonal antibody, clone OTI3F1 (formerly 3F1)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UFD1L (RC202989, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UFD1L. Mouse IgG1 OTI3F1 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

UFD1L antibody

  UFD1L mouse monoclonal antibody, clone OTI3A12 (formerly 3A12)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UFD1L (RC202989, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UFD1L. Mouse IgG3 OTI3A12 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

UFD1L antibody

  Purified UFD1L mouse monoclonal antibody, clone OTI3E9 (formerly 3E9)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UFD1L (RC202989, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UFD1L. Mouse IgG1 OTI3E9 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

UFD1L antibody

  UFD1L mouse monoclonal antibody, clone OTI4C7 (formerly 4C7)  
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-UFD1L mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804306) Mouse IgG1 OTI4C7 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

UFD1L antibody

  UFD1L mouse monoclonal antibody, clone OTI3B4 (formerly 3B4)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UFD1L (RC202989, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UFD1L. Mouse IgG1 OTI3B4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

UFD1L antibody

  UFD1L mouse monoclonal antibody, clone OTI5E3 (formerly 5E3)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UFD1L (RC202989, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UFD1L. Mouse IgG1 OTI5E3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CRISPR

Catalog No. Species Donor Vector  

UFD1L - human gene knockout kit via CRISPR

UFD1L - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

UFD1L - human gene knockout kit via CRISPR

UFD1L - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

UFD1L - human gene knockout kit via CRISPR

UFD1L - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

UFD1L - human gene knockout kit via CRISPR

UFD1L - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

UFD1L (transcript variant 1)

  Recombinant protein of human ubiquitin fusion degradation 1 like (yeast) (UFD1L), transcript variant 1  
UFD1L Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

UFD1L (transcript variant 2)

  Recombinant protein of human ubiquitin fusion degradation 1 like (yeast) (UFD1L), transcript variant 2  
UFD1L Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

UFD1L (1-307, His-tag)

UFD1L Human Purified > 85 % by SDS - PAGE E. coli
0.25 mg / €940.00
  Acris Antibodies GmbH

UFD1L (1-307, His-tag)

UFD1L Human Purified > 85 % by SDS - PAGE E. coli
50 µg / €370.00
  Acris Antibodies GmbH

Lysates

Catalog No.    

UFD1L overexpression lysate

UFD1L overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, UFD1L (mGFP-tagged) - Human ubiquitin fusion degradation 1 like (yeast) (UFD1L), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, UFD1L (mGFP-tagged) - Human ubiquitin fusion degradation 1 like (yeast) (UFD1L), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €960.00
  OriGene Technologies, Inc.

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