UFD1L antibody

Principal name

UFD1L antibody

Alternative names for UFD1L antibody

UB fusion protein 1, Ubiquitin fusion degradation protein 1 homolog

SwissProt ID

P70362 (Mouse), Q92890 (Human)

Gene ID

7353 (UFD1L)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of UFD1L antibody

The U box is a domain of approximately 70 amino acids that is present in proteins from yeast to humans. The prototype U box protein, yeast Ufd2, was identified as a ubiquitin chain assembly factor that cooperates with a ubiquitin-activating enzymes (E1, E2), and a ubiquitin-protein ligase (E3) to catalyze ubiquitin chain formation on artificial substrates. UFD1L is an essential component of the ubiquitin-dependent proteolytic pathway which degrades ubiquitin fusion proteins. The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteosome. In mammalian cells the membrane bound ubiquitin ligase gp75 promotes degradation of sterol regulated degradation of HMG CoA reductase and misfolded endoplasmic reticulum proteins. The ubiquitin fusion degradation 1 like (UFD1L) protein plays a critical role in ER associated degradation in association with Npl4 and VCP proteins. Interaction of VCP-UFD1L-Npl4 complex recognizes polyubiquitin chains and transfers the ubiquitinated proteins to proteosomes. The UFD1L also directly interacts with membrane bound gp75 as a cofactor and accelerate the ubiquitination and degradation of reductase and eventually promotes receptor mediated uptake of low density lipoproteins, thus plays an important role in cholesterol metabolism (1). It is shown that UFD1L-Npl4 complex is a negative regulator of retrotranslocation of A1 chain of the cholera toxin (CT) to the cytosol across the ER, delaying the retro-translocation of ERAD substrates independently of its association with VCP (2). The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. It may be involved in the development of some ectoderm-derived structures.
UFD1L gene is located on chromosome22q1.2 and is expressed during embryogenesis in the eyes and in the linear ear primordial. UFD1L gene is expressed in patients with DiGeorge (DGS) and velo-cardial facial (VCF) syndrome which suggest that proteolytic pathways that recognize ubiquitin fusion proteins for degrradtioin is conserved in vertebrates and UFD1L gene hemizygosity is the cause of some of the CATCH-22 (acronym for cardiac defects, abnormal facies, thymic hypoplasia, cleft palate and hypocalcimea) associated developmental defects in DGS and VCSF syndromes (3). UFD1L is expressed in adult heart, skeletal muscle and pancreas, and in fetal liver and kidney. UFD1L is a 307 amino acid protein apparent MW is 38kDa.

General readings

1. Cao J, Wang J, Qi W, Miao HH, Wang J, Ge L, DeBose-Boyd RA, Tang JJ, Li BL, Song BL. Cell Metab. 2007 Aug;6(2):115-28.
2. .McConnell E, Lass A, Wójcik C. Biochem Biophys Res Commun. 2007 Apr 20;355(4):1087-90.
3. Pizzuti A, Novelli G, Ratti A, Amati F, Mari A, Calabrese G, Nicolis S, Silani V, Marino B, Scarlato G, Ottolenghi S, Dallapiccola B. Hum Mol
Genet. 1997 Feb;6(2):259-65.

2 Item(s)

per page

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

UFD1L (transcript variant 1)

  Recombinant protein of human ubiquitin fusion degradation 1 like (yeast) (UFD1L), transcript variant 1  
UFD1L Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

UFD1L (transcript variant 2)

  Recombinant protein of human ubiquitin fusion degradation 1 like (yeast) (UFD1L), transcript variant 2  
UFD1L Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

2 Item(s)

per page
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