UGPase antibody

Principal name

UGPase antibody

Alternative names for UGPase antibody

UDP-glucose pyrophosphorylase, UDPGP, UTP-glucose-1-phosphate uridylyltransferase

SwissProt ID

Q43772 (Horvu)

Ncbi ID

4513

Available reactivities

Arabidopsis

Available hosts

Rabbit

Available applications

Western blot / Immunoblot (WB)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

UGPase (cytoplasm marker) antibody

15 µg of  total soluble protein  extract from leaves and stems of  Solanum tuberosum (1),  Solanum sogarandinum (2),   Lycopersicum esculentum (3),  Lycopersicum chilense  (4) , Arabidopsis thaliana (5) , Cucumis sativus (6) ,  Festuca arundinacea  (7) , Nicotiana tabacum (8) and Capsicum annuum (9)  were separated  on 10% SDS-PAGE and blotted onto nitrocellulose .  After blocking with 5% milk in TBST , blots were incubated with the primary antibody at a dilution of 1:1500 in TBST for 1h  at room temperature. Following incubation and wash steps, blots were incubated withSIGMA secondary Anti-Rabbit IgG , Alkaline Phosphatase Conjugate for 1 hour at a dilution of 1:40000 . Blots were developed with the alkaline phosphatase detection system using NBT/BCIP (SIGMA). Courtesy of Bartosz Szabala, Institute of Plant Genetics, Polish Academy of Science . Rabbit Serum all eucaryotic cells, Arabidopsis WB
50 µl / €300.00
  AgriSera AB

UGPase (Cytoplasm marker) (Hordeum vulgare) antibody

10 µg of total protein from Arabidopsis thaliana leaf (1) , Hordeum vulgare leaf (2), Zea mays leaf (3), recombinant UGPase 0.5 pmol (4),  were extracted with Protein Extraction Buffer PEB (AS08 300). Samples were diluted with 1X sample buffer (NuPAGE LDS sample buffer (Invitrogen) supplemented with 50 mM DTT and heat at 70°C for 5 min and keept on ice before loading. Protein samples were separated on 4-12% Bolt Plus gels,  LDS-PAGE and blotted for 70 minutes to PVDF using tank transfer. Blots were blocked immediately following transfer in 2% blocking reagent (GE RPN 2125; Healthcare) or 5% non-fat milk dissolved in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 10 000 (in blocking reagent) for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, and then washed 1x15 min and 3x5 min with TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, recommended secondary antibody AS09 602, Agrisera) diluted to 1:25 000 in blocking reagent for 1h at room temperature with agitation. The blots were washed as above. The blot was developed for 5 min with detection reagent according the manufacturers instructions. Images of the blots were obtained using a CCD imager (VersaDoc MP 4000) and Quantity One software (Bio-Rad). Exposure time was 10 seconds. Rabbit Serum Arabidopsis, Arabidopsis thaliana, Hordeum vulgare, Zea mays WB
50 µl / €300.00
  AgriSera AB

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