UHRF1 antibody
Principal name
UHRF1 antibody
Alternative names for UHRF1 antibody
ICBP90, NP95, RNF106, E3 ubiquitin-protein ligase UHRF1, Ubiquitin-like PHD and RING finger domain-containing protein 1, Ubiquitin-like-containing PHD and RING finger domains protein 1, Inverted CCAAT box-binding protein of 90 kDa, Nuclear zinc finger protein Np95, RING finger protein 106
Ncbi ID
9606, NP_037414, NP_001041666.1, ABZ92323.1, NM_001048201, NP_001104548.1, NM_001111078, NM_010931, NM_001111079, NM_001111080
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F)
Background of UHRF1 antibody
- Kit Component:
- - KN318685G1, Uhrf1 gRNA vector 1 in pCas-Guide vector
- KN318685G2, Uhrf1 gRNA vector 2 in pCas-Guide vector
- KN318685D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Immunoperoxidase of monoclonal antibody to UHRF1 on formalin-fixed paraffin-embedded human endometrium. [antibody concentration 3 ug/ml]
Immunofluorescence of monoclonal antibody to UHRF1 on HeLa cell. [antibody concentration 10 ug/ml]
UHRF1 monoclonal antibody (M01), clone 3A11 Western Blot analysis of UHRF1 expression in HeLa NE ( Cat # L013V3 ).
Immunofluorescence of monoclonal antibody to UHRF1 on MCF-7 cell . [antibody concentration 10 ug/ml]
UHRF1 monoclonal antibody (M02), clone 3B12 Western Blot analysis of UHRF1 expression in MCF-7 ( Cat # L046V1 ).
Detection limit for recombinant GST tagged UHRF1 is approximately 1ng/ml as a capture antibody.
HCT116 + siRNA; WB Suggested Anti-UHRF1 Antibody . . Positive Control: Lane 1: 60ug HCT116 lysate Lane 2: 60ug HCT116 lysate + siRNA . Primary Antibody Dilution : 1:1000. Secondary Antibody : Anti rabbit-HRP . Secondry Antibody Dilution : 1:5,000. Submitted by: Chinweike Ukomadu, Brigham and Women's Hospital, Boston; UHRF1 antibody - N-terminal region (ARP39021_P050) in HCT116 + siRNA cells using Western Blot
Human Stomach; WB Suggested Anti-UHRF1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Stomach; UHRF1 antibody - N-terminal region (ARP39021_P050) in Human Stomach cells using Western Blot
Western blot analysis of UHRF1 expression in HeLa (A); HCT116 (B); HEK293 (C) whole cell lysates.
UHRF1 Antibody (Center) (Cat. #TA325077) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the UHRF1 antibody detected the UHRF1 protein (arrow).
IF image of Hela cell stained with UHRF1 Antibody (Center)(Cat#TA325077).Hela cells were incubated with UHRF1 primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) . UHRF1 immunoreactivity is localized to Nucleus significantly.
UHRF1 Antibody (Center) (Cat.#TA325077) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of extracts of various cell lines, using UHRF1 antibody.
Immunofluorescence analysis of U20S cell using UHRF1 antibody. Blue: DAPI for nuclear staining.
WB Suggested Anti-UHRF1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human Stomach
WB Suggested Anti-UHRF1 Antibody; Positive Control: Lane 1: 60ug HCT116 lysate Lane 2: 60ug HCT116 lysate + siRNA; Primary Antibody Dilution: 1: 1000; Secondary Antibody: Anti rabbit-HRP; Secondry Antibody Dilution: 1: 5, 000; Submitted by: Chinweike Ukom
Western blot detection of UHRF1 in Mouse heart,293T,HelaNE and Hela cell lysates using UHRF1 mouse mAb (1:1000 diluted).Predicted band size: 90KDa.Observed band size: 90KDa.
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP314251 | UHRF1 (transcript variant 2) |
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| Recombinant protein of human ubiquitin-like with PHD and ring finger domains 1 (UHRF1), transcript variant 2 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
