ULK1 / ATG1 antibody

Principal name

ULK1 / ATG1 antibody

Alternative names for ULK1 / ATG1 antibody

KIAA0722, Unc-51-like kinase 1, Autophagy-related protein 1 homolog, Serine/threonine-protein kinase ULK1

SwissProt ID

O70405 (Mouse), O75385 (Human)

Gene ID

8408 (ULK1), 22241 (Ulk1)

Available reactivities

Hu (Human), Mky (Monkey), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), Por (Porcine), Ze (Zebrafish), Prim (Primate)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F)

Background of ULK1 / ATG1 antibody

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). Two human homologs of the yeast autophagy-specific kinase exist: ULK1(APG1) and ULK2. APG1 plays a critical role in regulating key elements of the autophagy pathway. APG1 stimulates autophagy, leading to autophagy-dependent restriction of cell growth and ultimately cell apoptosis at high levels of activity, and is a negative regulator of mTOR signaling.

General readings

Greenman C., et.al., Nature 446:153-158 (2007).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ULK1 / ATG1 antibody

  Rabbit Polyclonal ULK1 Antibody  
Western blot analysis of ULK1 in rat brain tissue lysate with ULK1 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ULK1 / ATG1 (649-679) antibody

  Rabbit polyclonal APG1 (ULK1) Antibody (Center)  
Western blot analysis of anti-ULK1 Pab (Cat. #TA325024) in A2058 cell lysate. ULK1 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. Rabbit Ig Aff - Purified Hu WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

ULK1 / ATG1 antibody

  Rabbit Polyclonal Anti-ULK1 Antibody  
Host: Rabbit; Target Name: ULK1; Sample Tissue: Thymus Tumor lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Can, Eq, Hu, Por, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

ULK1 / ATG1 antibody

  Rabbit Polyclonal ULK1 Antibody  
Western Blot: ULK1 Antibody [TA337050] - Analysis of ULK1 in Raji cell lysate in the 1) absence, 2) presence of immunizing peptide and 3) RAW using this antibody. Rabbit IgG Purified Hu, Ms, Prim P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ULK1 / ATG1 (320-370) antibody

Kidney: Formalin-Fixed Paraffin Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Mky P, WB
50 µg / €470.00
  Acris Antibodies GmbH

ULK1 / ATG1 antibody

Formalin-fixed and paraffin-embedded human skeletal muscle reacted with Denatured ATG1 Antibody Cat.-No AP51239PU-N, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu, Ms F, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

ULK1 - human gene knockout kit via CRISPR

ULK1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

ULK1 - human gene knockout kit via CRISPR

ULK1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

ULK1 - human gene knockout kit via CRISPR

ULK1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

ULK1 - human gene knockout kit via CRISPR

ULK1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Ulk1 - mouse gene knockout kit via CRISPR

Ulk1 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Ulk1 - mouse gene knockout kit via CRISPR

Ulk1 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Ulk1 - mouse gene knockout kit via CRISPR

Ulk1 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Ulk1 - mouse gene knockout kit via CRISPR

Ulk1 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

ULK1 / ATG1

  Recombinant protein of human unc-51-like kinase 1 (C. elegans) (ULK1)  
ULK1 / ATG1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

ULK1 overexpression lysate

ULK1 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, ULK1 (mGFP-tagged) - Human unc-51-like kinase 1 (C. elegans) (ULK1), 200 uL, >10^7 TU/mL

Lenti ORF particles, ULK1 (mGFP-tagged) - Human unc-51-like kinase 1 (C. elegans) (ULK1), 200 uL, >10^7 TU/mL  
200 µl / €2,120.00
  OriGene Technologies, Inc.

Lenti ORF particles, ULK1 (Myc-DDK tagged) - Human unc-51-like kinase 1 (C. elegans) (ULK1) , 200 uL, >10^7 TU/mL

Lenti ORF particles, ULK1 (Myc-DDK tagged) - Human unc-51-like kinase 1 (C. elegans) (ULK1) , 200 uL, >10^7 TU/mL  
200 µl / €1,910.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Ulk1 (GFP-tagged) - Mouse Unc-51 like kinase 1 (C. elegans) (Ulk1), 200 uL, >10^7 TU/mL

Lenti ORF particles, Ulk1 (GFP-tagged) - Mouse Unc-51 like kinase 1 (C. elegans) (Ulk1), 200 uL, >10^7 TU/mL  
200 µl / €1,840.00
  OriGene Technologies, Inc.

Lenti ORF particles, Ulk1 (Myc-DDK-tagged) - Mouse Unc-51 like kinase 1 (C. elegans) (Ulk1), 200 uL, >10^7 TU/mL

Lenti ORF particles, Ulk1 (Myc-DDK-tagged) - Mouse Unc-51 like kinase 1 (C. elegans) (Ulk1), 200 uL, >10^7 TU/mL  
200 µl / €1,660.00
  OriGene Technologies, Inc.

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