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ULK1 / ATG1 antibody

Principal name

ULK1 / ATG1 antibody

Alternative names for ULK1 / ATG1 antibody

KIAA0722, Unc-51-like kinase 1, Autophagy-related protein 1 homolog, Serine/threonine-protein kinase ULK1

SwissProt ID

O70405 (Mouse), O75385 (Human)

Gene ID

8408 (ULK1), 22241 (Ulk1)

Ncbi ID

9606, NP_003556.1, NP_003556, NM_003565, NP_033495.2, NM_009469

Available reactivities

Hu (Human), Mky (Monkey), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), Por (Porcine), Ze (Zebrafish), Prim (Primate)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F)

Background of ULK1 / ATG1 antibody

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). Two human homologs of the yeast autophagy-specific kinase exist: ULK1(APG1) and ULK2. APG1 plays a critical role in regulating key elements of the autophagy pathway. APG1 stimulates autophagy, leading to autophagy-dependent restriction of cell growth and ultimately cell apoptosis at high levels of activity, and is a negative regulator of mTOR signaling.

General readings

Greenman C., et.al., Nature 446:153-158 (2007).

ULK1 monoclonal antibody (M01), clone 2H8. Western Blot analysis of ULK1 expression in human colon.

H00008408-M01

Detection limit for recombinant GST tagged ULK1 is approximately 0.03ng/ml as a capture antibody.

H00008408-M01

Kidney: Formalin-Fixed Paraffin Embedded (FFPE)

AP08293PU-N

Formalin-fixed and paraffin-embedded human skeletal muscle reacted with Denatured ATG1 Antibody Cat.-No AP51239PU-N, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

AP51239PU-N

Western blot analysis of Denatured ATG1 Antibody Cat.-No AP51239PU-N in mouse cerebellum tissue lysates (35ug/lane). ATG1 (arrow) was detected using the purified Pab.

AP51239PU-N

Flow cytometric analysis of k562 cells using Denatured ATG1 Antibody Cat.-No AP51239PU-N (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

AP51239PU-N

TP315643

Western blot analysis of ULK1 in rat brain tissue lysate with ULK1 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.

TA319746

Immunohistochemistry of ULK1 in rat brain tissue with ULK1 antibody at 2.5 ug/mL.

TA319746

Immunofluorescence of ULK1 in rat brain tissue with ULK1 antibody at 20 ug/mL.

TA319746

Western blot analysis of anti-ULK1 Pab (Cat. #TA325024) in A2058 cell lysate. ULK1 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

TA325024

IF image of U251 cells stained with APG1 (ULK1) (Center) antibody. U251 cells were treated with Chloroquine , then incubated with TA325024 APG1 (ULK1) (Center) primary antibody (1:100, 2 h at RT). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) . APG1 (ULK1) immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.

TA325024

Host: Rabbit; Target Name: ULK1; Sample Tissue: Thymus Tumor lysates; Antibody Dilution: 1.0 ug/ml

TA335767

Western Blot: ULK1 Antibody [TA337050] - Analysis of ULK1 in Raji cell lysate in the 1) absence, 2) presence of immunizing peptide and 3) RAW using this antibody.

TA337050

Immunohistochemistry-Paraffin: ULK1 Antibody [TA337050] - IHC analysis of human kidney using TA337050 at 10 ug/ml.

TA337050

Primary Antibodies

Catalog No.	Host	Iso.	Pres.	React.	Applications
TA319746	ULK1 / ATG1 antibody
	Rabbit Polyclonal ULK1 Antibody
	Rabbit	IgG	Purified	Hu, Ms, Rt	WB	0.1 mg / €415.00
						OriGene Technologies, Inc.
+2 additional images
TA325024	ULK1 / ATG1 (649-679) antibody
	Rabbit polyclonal APG1 (ULK1) Antibody (Center)
	Rabbit	Ig	Aff - Purified	Hu	WB	0.4 ml / €415.00
						OriGene Technologies, Inc.
+1 additional image

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