Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Por (Porcine), Mky (Monkey), Rb (Rabbit)
Available hosts
Mouse, Rabbit
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F)
Background of Uracil-DNA glycosylase (UNG) antibody
The human UNG gene encodes both nuclear (UNG2) and mitochondrial (UNG1) forms of uracil-D glycosylase. These forms are generated by altertive splicing and the use of two differentially regulated promoters, PA and PB (Slupphaug et al., 1993; Nilsen et al., 1997) The cDs for UNG1 and UNG2 are of similar size (2061 and 2058 bp, respectively) and as a result the corresponding mRs are not resolved as two species in gel electrophoresis (Huag et al., 1998). Nuclear UNG2 differs from mitochondrial UNG1 in 44 amino acids of the N-termil sequence that is not necessary for catalytic activity. A major role of the UNG gene products is to repair mutagenic U:G mispairs caused by cytosine deamition. For example, UNG2 removes misincorporated dUMP residues. The level and expression pattern of UNG1 and UNG2 differs between cell and tissue type (Huag et al. 1998). Additiolly, the expression of UNG is cell cycle regulated (gelhus et al. 1995). The expression and activity of UNG has been found in general to be higher in proliferating as compared to nonproliferating tissues and cells (reviewed in Kruman et al. 2004). UNG1 is a 304 amino acid protein. UNG2 is a 313 amino acid protein.
Formalin-Fixed Paraffin-Embedded Human Kidney stained with Uracil-DNA glycosylase / UNG Antibody Cat.-No AP07386PU-N at 5 µg/ml after heat-induced antigen retrieval.
Western blot analysis of whole cell lysate from C2C12 mouse myoblast cells probed with Rabbit anti Human uracil-DNA glycosylase (AP05613PU-N) at 0.5(A), 1(B) and 2(C) µg/ml
Western blot analysis of cell lysate of HeLa ( 30 µg ) were resolved by SDS - PAGE , transferred to PVDF membrane and probed with UNG monoclonal antibody , clone k1C12 ( 1 : 1000 ) ( Cat # MAB1099 ) . Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system.
Western Blot analysis of UNG expression in transfected 293T cell line (H00007374-T01) by UNG MaxPab polyclonal antibody.Lane 1: UNG transfected lysate(33.44 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry: AM09040PU-N UNG antibody staining of Formalin-Fixed, Paraffin-Embedded Human Thymus at 10 µg/ml followed by biotinylated anti-Mouse IgG secondary antibody, Alkaline Phosphatase-Streptavidin and chromogen.
Immunohistochemistry: AM09040PU-N UNG antibody staining of Formalin-Fixed, Paraffin-Embedded Human Testis at 10 µg/ml followed by biotinylated anti-Mouse IgG secondary antibody, Alkaline Phosphatase-Streptavidin and chromogen.
Immunohistochemistry: AM09040PU-N UNG antibody staining of Formalin-Fixed, Paraffin-Embedded Human Heart at 10 µg/ml followed by biotinylated anti-Mouse IgG secondary antibody, Alkaline Phosphatase-Streptavidin and chromogen.
Western blot analysis: Cell lysates of HeLa (30 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human UNG (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Cell lysates of HeLa (30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human UNG (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Western Blot analysis of UNG expression in transfected 293T cell line (H00007374-T01) by UNG MaxPab polyclonal antibody.Lane 1: UNG transfected lysate(33.90 KDa).Lane 2: Non-transfected lysate.
Human HeLa; WB Suggested Anti-UNG Antibody. . Titration: 1.0 ug/ml. . Positive Control: Hela Whole Cell. UNG is supported by BioGPS gene expression data to be expressed in HeLa.; UNG antibody - C-terminal region (ARP61205_P050) in Human HeLa cells using Western Blot
Immunohistochemical analysis of UDG staining in human muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-UNG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503499)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-UNG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503499)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-UNG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503499)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UNG (RC222868, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UNG.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UNG (RC222868, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UNG.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-UNG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503513)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-UNG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503513)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-UNG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503513)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-UNG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503513)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-UNG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503513)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UNG (RC222868, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UNG.
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-UNG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503533)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-UNG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503533)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UNG (RC222868, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UNG.
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-UNG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503534)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-UNG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503534)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-UNG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503534)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UNG (RC222868, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UNG.
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-UNG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503563)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-UNG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503563)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UNG (RC222868, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UNG.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UNG (RC222868, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UNG.
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-UNG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503613)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-UNG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503613)
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-UNG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503613)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UNG (RC222868, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UNG.
HEK293T cells transfected with either RC222868 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-UNG antibody(TA503613), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-UNG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503618)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UNG (RC222868, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UNG.
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-UNG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503755)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-UNG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503755)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-UNG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503755)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UNG (RC222868, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UNG.
Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-UNG monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
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