URM1 antibody

Principal name

URM1 antibody

Alternative names for URM1 antibody

URM-1, Ubiquitin-related modifier 1, C9orf74, YIL008W

SwissProt ID

P40554 (Yeast), Q148F0 (Bovin), Q9BTM9 (Human), Q9D2P4 (Mouse)

Gene ID

81605 (URM1), 854809

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), S. cerevisiae (Saccharomyces cerevisiae), Bac (Bacteria), Ye (Yeast)

Available hosts

Rabbit

Available applications

Enzyme Immunoassay (E), Paraffin Sections (P), Western blot / Immunoblot (WB), Frozen Sections (C)

Background of URM1 antibody

Ubiquitin-like proteins fall into two classes: the first class, ubiquitin-like modifiers (UBLs) function as modifiers in a manner analogous to that of ubiquitin. Examples of UBLs are SUMO, Rub1 (also called Nedd8), Apg8 and Apg12. Proteins of the second class include parkin, RAD23 and DSK2, are designated ubiquitin-domain proteins (UDPs). These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other. In contrast to UBLs, UDPs are not conjugated to other proteins. Urm1 is a newly identified ubiquitin related modifier. Urm 1 is a 99-amino acid protein terminated with glycine-glycine. Target proteins are conjugated to Urm1 via its C-terminal glycine. Initially Urm1 forms a thioester with a novel E1-like protein, Uba4.

General readings

Furukawa K et al. A protein conjugation system in yeast with homology to biosynthetic enzyme reaction of prokaryotes. J Biol Chem 275:7462-5 (2000). PubMed PMID: 10713047
Hochstrasser M Evolution and function of ubiquitin-like protein-conjugation systems. Nat Cell Biol 2:E153-7 (2000). PubMed PMID: 10934491
Jentsch S & Pyrowolakis G Ubiquitin and its kin: how close are the family ties? Trends Cell Biol 10:335-42 (2000). PubMed PMID: 10884686

11 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

URM1 antibody

Western blot analysis of hURM1 (arrow) using rabbit polyclonal hURM1 Antibody (N-term) (Cat.#TA302028). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the hURM1 gene (Lane 2) (Origene Technologies). Rabbit Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

URM1 antibody

Figure 1. Immunoblot of Urm1 fusion protein: Anti- Urm1 antibody generated by immunization with recombinant yeast Urm1 was tested by immunoblot against yeast lysates expressing the Urm1-GFP fusion protein and other UBL fusion proteins. All UBLs possess limited homology to Ubiquitin and to each other, therefore it is important to know the degree of reactivity of each antibody against each UBL. Panel A shows total protein staining using ponceau. Panel B shows positions of free GFP or GFP containing recombinant proteins present in each lysate preparation after reaction with a 1:1,000 dilution of anti-GFP followed by reaction with a 1:15,000 dilution of HRP Donkey-a-Goat IgG. Panel C shows specific reaction with Urm1 using a 1:1,000 dilution of IgG fraction of Rabbit-anti- Urm1 (Yeast) followed by reaction with a 1:15,000 dilution of HRP Goat-a-Rabbit IgG. All primary antibodies were diluted in TTBS buffer supplemented with 5% non-fat milk and incubated with the membranes overnight at 4° C. Yeast lysate proteins were separated by SDS-PAGE using a 15% gel. This data indicates that anti-Urm1 is highly specific and does not cross react with other UBLs. Bands present in Panel C indicate that Urm1 and conjugated Urm1 is present in most yeast cell lysates albeit at significantly reduced levels relative to the Urm1-GFP transfected lysate. A chemiluminescence system was used for signal detection (Roche). Other detection systems will yield similar results. Data contributed by M. Malakhov, www.lifesensors.com, personal communication. Rabbit Purified Ye E, WB
0.5 mg / €440.00
  Acris Antibodies GmbH

URM1 antibody

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with URM1 polyclonal antibody ( Cat # PAB3628 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. Rabbit Hu P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

URM1 antibody

Immunoblot of Urm1 fusion protein.
URM1 polyclonal antibody ( Cat # PAB11366 ) generated by immunization with recombinant yeast URM1 was tested by immunoblot against yeast lysates expressing the URM1-GFP fusion protein and other UBL fusion proteins.
All UBLs possess limited homology to Ubiquitin and to each other, therefore it is important to know the degree of reactivity of each antibody against each UBL.
Panel A shows total protein staining using ponceau.
Panel B shows positions of free GFP or GFP containing recombinant proteins present in each lysate preparation after reaction with a 1:1,000 dilution of anti-GFP followed by reaction with a 1:15,000 dilution of HRP Donkey-a-Goat IgG MX.
Panel C shows specific reaction with URM1 using a 1:1,000 dilution of URM1 polyclonal antibody ( Cat # PAB11366 ) ( Yeast ) followed by reaction with a 1:15,000 dilution of HRP Goat-a-Rabbit IgG MX.
All primary antibodies were diluted in TTBS buffer supplemented with 5% non-fat milk and incubated with the membranes overnight at 4°C.
Yeast lysate proteins were separated by SDS-PAGE using a 15% gel. This data indicates that anti-URM1 is highly specificand does not cross react with other UBLs.
Bands present in Panel C indicate that URM1 and conjugated URM1 is present in most yeast cell lysates albeit at significantly reduced levels relative to the URM1-GFP transfected lysate.
A chemiluminescence system was used for signal detection ( Roche ) .
Data contributed by M. Malakhov, www.lifesensors.com, personal communication. Rabbit Ye E, WB
0.1 mg / €560.00
  Abnova Taiwan Corp.

URM1 antibody

Western Blot: URM1 Antibody [NB600-469] - Immunoblot of Urm1 fusion protein. Anti- Urm1 antibody generated by immunization with recombinant yeast Urm1 was tested by immunoblot against yeast lysates expressing the Urm1-GFP fusion protein and other UBL fusion proteins. All UBLs possess limited homology to Ubiquitin and to each other, therefore it is important to know the degree of reactivity of each antibody against each UBL. Panel A shows total protein staining using ponceau. Panel B shows positions of free GFP or GFP containing recombinant proteins present in each lysate preparation after reaction with a 1:1,000 dilution of anti-GFP followed by reaction with a 1:15,000 dilution of HRP Donkey-a-Goat IgG MX. Panel C shows specific reaction with Urm1 using a 1:1,000 dilution of IgG fraction of Rabbit-anti- Urm1 (Yeast) followed by reaction with a 1:15,000 dilution of HRP Goat-a-Rabbit IgG MX. All primary antibodies were diluted in TTBS buffer supplemented with 5% non-fat milk and incubated with the membranes overnight at 4 degrees C. Yeast lysate proteins were separated by SDS-PAGE using a 15% gel. This data indicates that anti-Urm1 is highly specific and does not cross react with other UBLs. Bands present in Panel C indicate that Urm1 and conjugated Urm1 is present in most yeast cell lysates albeit at significantly reduced levels relative to the Urm1-GFP transfected lysate. A chemiluminescence system was used for signal detection (Roche). Other detection systems will yield similar results. Data contributed by M. Malakhov, www.lifesensors.com, personal communication. Rabbit Ig Fraction Bac E, WB
0.5 mg / €510.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

URM1 (transcript variant 1)

URM1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

URM1 (1-101, His-tag)

URM1 Human Purified > 95 % E. coli
0.5 mg / €750.00
  Acris Antibodies GmbH

URM1 (1-101, His-tag)

URM1 Human Purified > 95 % E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

URM1

URM1 Human
0.1 mg / €380.00
  Abnova Taiwan Corp.

URM1

URM1 Human Purified
0.1 mg / €380.00
  Novus Biologicals Inc.

URM1

URM1 Human Purified
0.5 mg / €870.00
  Novus Biologicals Inc.

11 Item(s)

per page
  • LinkedIn