URM1 antibody

Principal name

URM1 antibody

Alternative names for URM1 antibody

URM-1, Ubiquitin-related modifier 1, C9orf74, YIL008W

SwissProt ID

P40554 (Yeast), Q148F0 (Bovin), Q9BTM9 (Human), Q9D2P4 (Mouse)

Gene ID

854809 (URM1), 81605, 68205 (Urm1)

Available reactivities

Ye (Yeast), Hu (Human)

Available hosts

Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P)

Background of URM1 antibody

Kit Component:
- KN318751G1, Urm1 gRNA vector 1 in pCas-Guide vector
- KN318751G2, Urm1 gRNA vector 2 in pCas-Guide vector
- KN318751D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

Xu J., et al. (2006) Proc Natl Acad Sci U S A. 103(31):11625-30.
Schlieker CD., et al. (2008) Proc Natl Acad Sci U S A. 105(47):18255-60.

18 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

URM1 antibody

  Rabbit Polyclonal Antibody against URM1 (N-term)  
Western blot analysis of hURM1 (arrow) using rabbit polyclonal hURM1 Antibody (N-term) (Cat.#TA302028). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the hURM1 gene (Lane 2) (Origene Technologies). Rabbit Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

URM1 antibody

Figure 1. Immunoblot of Urm1 fusion protein: Anti- Urm1 antibody generated by immunization with recombinant yeast Urm1 was tested by immunoblot against yeast lysates expressing the Urm1-GFP fusion protein and other UBL fusion proteins. All UBLs possess limited homology to Ubiquitin and to each other, therefore it is important to know the degree of reactivity of each antibody against each UBL. Panel A shows total protein staining using ponceau. Panel B shows positions of free GFP or GFP containing recombinant proteins present in each lysate preparation after reaction with a 1:1,000 dilution of anti-GFP followed by reaction with a 1:15,000 dilution of HRP Donkey-a-Goat IgG. Panel C shows specific reaction with Urm1 using a 1:1,000 dilution of IgG fraction of Rabbit-anti- Urm1 (Yeast) followed by reaction with a 1:15,000 dilution of HRP Goat-a-Rabbit IgG. All primary antibodies were diluted in TTBS buffer supplemented with 5% non-fat milk and incubated with the membranes overnight at 4° C. Yeast lysate proteins were separated by SDS-PAGE using a 15% gel. This data indicates that anti-Urm1 is highly specific and does not cross react with other UBLs. Bands present in Panel C indicate that Urm1 and conjugated Urm1 is present in most yeast cell lysates albeit at significantly reduced levels relative to the Urm1-GFP transfected lysate. A chemiluminescence system was used for signal detection (Roche). Other detection systems will yield similar results. Data contributed by M. Malakhov, www.lifesensors.com, personal communication. Rabbit Purified Ye E, WB
0.5 mg / €510.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

URM1 - human gene knockout kit via CRISPR

URM1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

URM1 - human gene knockout kit via CRISPR

URM1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

URM1 - human gene knockout kit via CRISPR

URM1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

URM1 - human gene knockout kit via CRISPR

URM1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Urm1 - mouse gene knockout kit via CRISPR

Urm1 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Urm1 - mouse gene knockout kit via CRISPR

Urm1 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Urm1 - mouse gene knockout kit via CRISPR

Urm1 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Urm1 - mouse gene knockout kit via CRISPR

Urm1 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

URM1 (transcript variant 1)

  Recombinant protein of human ubiquitin related modifier 1 homolog (S. cerevisiae) (URM1), transcript variant 1  
URM1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

URM1 (1-101, His-tag)

URM1 Human Purified > 95 % E. coli
0.5 mg / €750.00
  Acris Antibodies GmbH

URM1 (1-101, His-tag)

URM1 Human Purified > 95 % E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, URM1 (mGFP-tagged) - Human ubiquitin related modifier 1 (URM1), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, URM1 (mGFP-tagged) - Human ubiquitin related modifier 1 (URM1), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, URM1 (mGFP-tagged)-Human ubiquitin related modifier 1 (URM1), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, URM1 (mGFP-tagged)-Human ubiquitin related modifier 1 (URM1), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, URM1 (Myc-DDK tagged) - Human ubiquitin related modifier 1 (URM1), transcript variant 1 , 200 uL, >10^7 TU/mL

Lenti ORF particles, URM1 (Myc-DDK tagged) - Human ubiquitin related modifier 1 (URM1), transcript variant 1 , 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

Lenti ORF particles, URM1 (Myc-DDK-tagged)-Human ubiquitin related modifier 1 (URM1), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, URM1 (Myc-DDK-tagged)-Human ubiquitin related modifier 1 (URM1), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Urm1 (GFP-tagged) - Mouse ubiquitin related modifier 1 homolog (S. cerevisiae) (Urm1), 200 uL, >10^7 TU/mL

Lenti ORF particles, Urm1 (GFP-tagged) - Mouse ubiquitin related modifier 1 homolog (S. cerevisiae) (Urm1), 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

18 Item(s)

per page
  • LinkedIn