USP18 antibody

Principal name

USP18 antibody

Alternative names for USP18 antibody

Ubl carboxyl-terminal hydrolase 18, ISG43, Ubl thioesterase 18, ISG15-specific-processing protease, 43 kDa ISG15-specific protease

SwissProt ID

Q9UMW8 (Human), Q9WTV6 (Mouse)

Gene ID

11274 (USP18)

Available reactivities

Hu (Human), Chimp (Chimpanzee), Ms (Mouse), Por (Porcine), Orang-Utan, Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), Paraffin Sections (P), Immunoprecipitation (IP)

Background of USP18 antibody

USP18, a member of the deubiquitinating protease family of enzymes, removes ubiquitin adducts from a broad range of protein substrates.USP18, a member of the deubiquitinating protease family of enzymes, removes ubiquitin adducts from a broad range of protein substrates.[supplied by OMIM]. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-232 AC008079.24 80964-81195 233-495 AC008079.24 88531-88793 496-592 AC008079.24 91145-91241 593-738 AC008079.24 92763-92908 739-818 AC008079.24 98228-98307 819-965 AC008079.24 98863-99009 966-1061 AC008079.24 100817-100912 1062-1229 AC008079.24 101726-101893 1230-1361 AC008079.24 104123-104254 1362-1411 AC008079.24 104766-104815 1412-2037 AC008079.24 107745-108370

General readings

Yan,M., (2007) Blood 110 (1), 305-312

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

USP18 (40-49) antibody

  Rabbit polyclonal anti-UBP43 antibody  
WB using Anti-UBP43 antibody shows detection of a band ~43 kDa band (arrowhead) in lysates from HeLa nuclear extracts (lane 1) and whole cell lysates from HeLa (lane 2), A431 (lane 3, Jurkat (Lane 4) and HEK293 (lane 5). Approximately 18 µg of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-UBP43 antibody.The strong band between the 37kD and 50kD markers is expected to be UBP43. Rabbit IgG Aff - Purified Chimp, Hu, Orang-Utan E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

USP18 antibody

  Rabbit Polyclonal Anti-USP18 Antibody  
USP18 antibody - N-terminal region validated by WB using Mouse Brain lysate at 2 ug/ml. Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

USP18 antibody

Heart: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

USP18 antibody

Western Blot analysis of USP18 expression in transfected 293T cell line (H00011274-T01) by USP18 MaxPab polyclonal antibody.

Lane 1: USP18 transfected lysate(41.03 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

USP18 antibody

Immunofluorescence of purified MaxPab antibody to USP18 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Serum Hu E(detection), ICC/IF, WB
50 µl / €370.00
  Abnova Taiwan Corp.

USP18 antibody

Immunofluorescence of purified MaxPab antibody to USP18 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

USP18 antibody

Western Blot analysis of USP18 expression in transfected 293T cell line (H00011274-T04) by USP18 MaxPab polyclonal antibody.

Lane 1: USP18 transfected lysate(40.92 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

USP18 antibody

USP18 Rabbit Serum Hu E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

USP18 antibody

USP18 Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

USP18 antibody

Western blot using USP18 polyclonal antibody ( Cat # PAB10241 ) shows detection of a band ~43kDa band ( arrowhead ) believed to be to USP18 in lysates from HeLa nuclear extracts ( lane 1 ) and whole cell lysates from HeLa ( lane 2 ), A-431 ( lane 3 ), Jurkat ( lane 4 ) and HEK293 ( lane 5 ).
Approximately 18 µg of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1 : 500 dilution of USP18 polyclonal antibody.
Signal was detected using standard techniques.
The strong band between the 37 kDa and 50 kDa markers is expected to be USP18.
The other bands are probably non-specific. Chimp, Hu, Ms
0.1 mg / €560.00
  Abnova Taiwan Corp.

USP18 antibody

Mouse Brain; USP18 antibody - N-terminal region (ARP46253_P050) validated by WB using Mouse Brain lysate at 2ug/ml.; USP18 antibody - N-terminal region (ARP46253_P050) in Mouse Brain cells using Western Blot Rabbit Aff - Purified Hu, Por WB
50 µg / €350.00
  AVIVA Systems Biology

CRISPR

Catalog No. Species Donor Vector  

USP18 - human gene knockout kit via CRISPR

USP18 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

USP18 - human gene knockout kit via CRISPR

USP18 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

USP18 - human gene knockout kit via CRISPR

USP18 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

USP18 - human gene knockout kit via CRISPR

USP18 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

USP18

USP18 Human in vitro transl.
2 µg / €300.00
  Abnova Taiwan Corp.

USP18

USP18 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

USP18

USP18 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Antibody Pairs

Catalog No.    

USP18 (Human) IP-WB Antibody Pair

USP18 (Human) IP-WB Antibody Pair  
1 set / €710.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

USP18 293T Cell Transient Overexpression Lysate(Denatured)

USP18 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

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