USP18 antibody

Principal name

USP18 antibody

Alternative names for USP18 antibody

Ubl carboxyl-terminal hydrolase 18, ISG43, Ubl thioesterase 18, ISG15-specific-processing protease, 43 kDa ISG15-specific protease

SwissProt ID

Q9UMW8 (Human), Q9WTV6 (Mouse)

Gene ID

11274 (USP18)

Available reactivities

Hu (Human), Chimp (Chimpanzee), Ms (Mouse), Orang-Utan, Por (Porcine), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), Paraffin Sections (P), Immunoprecipitation (IP)

Background of USP18 antibody

USP18, a member of the deubiquitinating protease family of enzymes, removes ubiquitin adducts from a broad range of protein substrates.USP18, a member of the deubiquitinating protease family of enzymes, removes ubiquitin adducts from a broad range of protein substrates.[supplied by OMIM]. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-232 AC008079.24 80964-81195 233-495 AC008079.24 88531-88793 496-592 AC008079.24 91145-91241 593-738 AC008079.24 92763-92908 739-818 AC008079.24 98228-98307 819-965 AC008079.24 98863-99009 966-1061 AC008079.24 100817-100912 1062-1229 AC008079.24 101726-101893 1230-1361 AC008079.24 104123-104254 1362-1411 AC008079.24 104766-104815 1412-2037 AC008079.24 107745-108370

General readings

Tokarz,S., Berset,C., La Rue,J., Friedman,K., Nakayama,K., Nakayama,K., Zhang,D.E. and Lanker,S. (2004) The ISG15 isopeptidase UBP43
is regulated by proteolysis via the SCFSkp2 ubiquitin ligase. J. Biol. Chem. 279 (45), 46424-46430.
Puente,X.S., Sanchez,L.M., Overall,C.M. and Lopez-Otin,C. (2003) Human and mouse proteases: a comparative genomic approach. Nat.
Rev. Genet. 4 (7), 544-558.
Kang,D., Jiang,H., Wu,Q., Pestka,S. and Fisher,P.B. (2001) Cloning and characterization of human ubiquitin-processing protease-43 from
terminally differentiated human melanoma cells using a rapid subtraction hybridization protocol RaSH. Gene 267 (2), 233-242.
Schwer,H., Liu,L.Q., Zhou,L., Little,M.T., Pan,Z., Hetherington,C.J. and Zhang,D.E. (2000) Cloning and characterization of a novel human
ubiquitin-specific protease, a homologue of murine UBP43 (Usp18). Genomics 65 (1), 44-52.
Li,X.L., Blackford,J.A., Judge,C.S., Liu,M., Xiao,W., Kalvakolanu,D.V. and Hassel,B.A. (2000) RNase-L-dependent destabilization of
interferon-induced mRNAs. A role for the 2-5A system in attenuation of the interferon response. J. Biol. Chem. 275 (12), 8880-8888.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

USP18 (40-49) antibody

WB using Anti-UBP43 antibody shows detection of a band ~43 kDa band (arrowhead) in lysates from HeLa nuclear extracts (lane 1) and whole cell lysates from HeLa (lane 2), A431 (lane 3, Jurkat (Lane 4) and HEK293 (lane 5). Approximately 18 µg of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-UBP43 antibody.The strong band between the 37kD and 50kD markers is expected to be UBP43. Rabbit IgG Aff - Purified Chimp, Hu, Orang-Utan E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

USP18 antibody

USP18 antibody - N-terminal region validated by WB using Mouse Brain lysate at 2 ug/ml. Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

USP18 antibody

Heart: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

USP18 antibody

Western Blot analysis of USP18 expression in transfected 293T cell line (H00011274-T01) by USP18 MaxPab polyclonal antibody.

Lane 1: USP18 transfected lysate(41.03 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

USP18 antibody

Immunofluorescence of purified MaxPab antibody to USP18 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Serum Hu E(detection), ICC/IF, WB
50 µl / €370.00
  Abnova Taiwan Corp.

USP18 antibody

Immunofluorescence of purified MaxPab antibody to USP18 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

USP18 antibody

Western Blot analysis of USP18 expression in transfected 293T cell line (H00011274-T04) by USP18 MaxPab polyclonal antibody.

Lane 1: USP18 transfected lysate(40.92 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

USP18 antibody

USP18 Rabbit Serum Hu E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

USP18 antibody

USP18 Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

USP18 antibody

Western blot using USP18 polyclonal antibody ( Cat # PAB10241 ) shows detection of a band ~43kDa band ( arrowhead ) believed to be to USP18 in lysates from HeLa nuclear extracts ( lane 1 ) and whole cell lysates from HeLa ( lane 2 ), A-431 ( lane 3 ), Jurkat ( lane 4 ) and HEK293 ( lane 5 ).
Approximately 18 µg of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1 : 500 dilution of USP18 polyclonal antibody.
Signal was detected using standard techniques.
The strong band between the 37 kDa and 50 kDa markers is expected to be USP18.
The other bands are probably non-specific. Chimp, Hu, Ms
0.1 mg / €560.00
  Abnova Taiwan Corp.

USP18 antibody

Mouse Brain; USP18 antibody - N-terminal region (ARP46253_P050) validated by WB using Mouse Brain lysate at 2ug/ml.; USP18 antibody - N-terminal region (ARP46253_P050) in Mouse Brain cells using Western Blot Rabbit Aff - Purified Hu, Por WB
50 µg / €350.00
  AVIVA Systems Biology

USP18 antibody

Western Blot: USP18 Antibody [NB600-893] - Western blot using NB600-893 at 1/500. Rabbit IgG Aff - Purified Hu E, ICC/IF, WB
0.1 mg / €510.00
  Novus Biologicals Inc.

USP18 antibody

Western  Blot:  USP18 Antibody [NBP1-33675] – Western blot analysis of USP18 on IMR32 lysates using NBP1-33675 at a dilution of 1:1000. 30ug whole cell lysate loaded on 10% SDS PAGE gel. Rabbit IgG Hu P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

USP18 antibody

Immunohistochemistry-Paraffin: USP18 Antibody [NBP1-92566] - Immunohistochemical staining of human esophagus shows strong cytoplasmic and membranous positivity in superficial layers of squamous epithelial cells. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

USP18

USP18 Human in vitro transl.
2 µg / €300.00
  Abnova Taiwan Corp.

USP18

USP18 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

USP18

USP18 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Antibody Pairs

Catalog No.    

USP18 (Human) IP-WB Antibody Pair

USP18 (Human) IP-WB Antibody Pair  
1 set / €710.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

USP18 293T Cell Transient Overexpression Lysate(Denatured)

USP18 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

USP18 overexpression lysate

USP18 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

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