USP25 antibody

Principal name

USP25 antibody

Alternative names for USP25 antibody

Ubiquitin carboxyl-terminal hydrolase 25, USP21, Ubiquitin thioesterase 25, Ubiquitin-specific-processing protease 25, Deubiquitinating enzyme 25, USP on chromosome 21

SwissProt ID

P57080 (Mouse), Q9UHP3 (Human)

Gene ID

29761 (USP25)

Available reactivities

Hu (Human), Rt (Rat), Ms (Mouse)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P)

Background of USP25 antibody

Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.

12 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

USP25 antibody

Western blot analysis of lysate from Daudi cell line, using USP25 Antibody (Cat. #TA328144). TA328144 was diluted at 1:1000. A goat anti-mouse IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug. Mouse IgG1 1277CT376.106.171 Purified Hu WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

USP25 antibody

Western blot analysis of USP25 in mouse brain tissue lysate with USP25 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide. Rabbit IgG Purified Hu, Ms ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

USP25 (N-term) antibody

Western Blot analysis of anti-USP25 (N-term) Pab (Cat.#AP12045PU-N) to detect USP25 in USP25-transfected HeLa cell lysates. Transfection data is kindly provided by Dr. B. Pierrat from the Novartis Institute for Biomedical Research (Basel, Switzerland). Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

USP25 (C-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu, Rt P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

USP25 antibody

The USP25 polyclonal antibody ( Cat # PAB4213 ) is used in Western blot to detect USP25 in USP25-transfected HeLa cell lysates . Transfection data is kindly provided by Dr . B . Pierrat from the Novartis Institute for Biomedical Research ( Basel, Switzerland ) . Rabbit Hu E, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

USP25 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with USP25 polyclonal antibody ( Cat # PAB4214 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . BC = breast carcinoma . Rabbit Hu, Rt E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

USP25 antibody

Immunohistochemistry-Paraffin: USP25 Antibody [NBP1-80630] Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine cells. Rabbit IgG Aff - Purified Hu P
0.1 ml / €500.00
  Novus Biologicals Inc.

USP25 antibody

Immunohistochemistry-Paraffin: USP25 Antibody [NBP1-80631] - Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

USP25

USP25 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

USP25

USP25 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

USP25

USP25 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

USP25 overexpression lysate

USP25 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

12 Item(s)

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