USP3 antibody

Principal name

USP3 antibody

Alternative names for USP3 antibody

Ubiquitin carboxyl-terminal hydrolase 3, Ubiquitin thioesterase 3, Ubiquitin-specific-processing protease 3, Deubiquitinating enzyme 3

SwissProt ID

Q91W36 (Mouse), Q9Y6I4 (Human)

Gene ID

9960 (USP3)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Rt (Rat), African clawed frog, Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Por (Porcine)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Immunoprecipitation (IP), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of USP3 antibody

Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.

General readings

Puente,X.S., et al., (2003) Nat. Rev. Genet. 4 (7), 544-558

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

USP3 antibody

Immunohistochemistry-Paraffin: USP3 Antibody [NB100-77284] - FFPE section of human ovarian carcinoma.  Affinity purified rabbit anti-USP3 used at a dilution of 1:500. Detection: DAB staining using Immunohistochemistry Accessory Kit Rabbit IgG Aff - Purified Hu F, ICC/IF, IP, P
0.1 mg / €440.00
  Novus Biologicals Inc.

USP3 antibody

WB Suggested Anti-USP3 Antibody Titration: 0.2-1 ug/ml; Positive Control: Jurkat cell lysateUSP3 is supported by BioGPS gene expression data to be expressed in Jurkat Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

USP3 antibody

WB Suggested Anti-USP3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:12500; Positive Control: Jurkat cell lysateUSP3 is supported by BioGPS gene expression data to be expressed in Jurkat Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

USP3 (N-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

USP3 (C-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

USP3 antibody

USP3 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

USP3 antibody

Immunoperoxidase of purified MaxPab antibody to USP3 on formalin-fixed paraffin-embedded human cerebral cortex. [antibody concentration 3 ug/ml] Mouse Serum Hu E(detection), P, WB
50 µl / €370.00
  Abnova Taiwan Corp.

USP3 antibody

USP3 Mouse Purified Hu E(detection), P, WB
50 µg / €370.00
  Abnova Taiwan Corp.

USP3 antibody

USP3 Rabbit Serum Hu E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

USP3 antibody

USP3 MaxPab rabbit polyclonal antibody. Western Blot analysis of USP3 expression in mouse spleen. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

USP3 antibody

Western Blot analysis of USP3 expression in transfected 293T cell line by USP3 monoclonal antibody (M01), clone 1H2.

Lane 1: USP3 transfected lysate(58.9 KDa).
Lane 2: Non-transfected lysate. Mouse IgG2a 1H2 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

USP3 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with USP3 polyclonal antibody ( Cat # PAB3917 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. Rabbit Hu E, P
0.4 ml / €390.00
  Abnova Taiwan Corp.

USP3 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with USP3 polyclonal antibody ( Cat # PAB3918 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. Rabbit Hu E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

USP3 antibody

Human Jurkat; WB Suggested Anti-USP3 Antibody Titration: 0.2-1 ug/ml. Positive Control: Jurkat cell lysate. USP3 is supported by BioGPS gene expression data to be expressed in Jurkat.; USP3 antibody - N-terminal region (ARP33680_P050) in Human Jurkat cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

USP3 antibody

Human Jurkat; WB Suggested Anti-USP3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: Jurkat cell lysate. USP3 is supported by BioGPS gene expression data to be expressed in Jurkat.; USP3 antibody - middle region (ARP33681_P050) in Human Jurkat cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

USP3 antibody

Western Blot: USP3 Antibody [NB100-77283] - Detection of Human USP3 by Western Blot of  Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP,  20% of IP loaded) from HeLa cells. Antibodies: Affinity purified  rabbit anti-USP3 antibody NB100-77283 used for IP at 3 mcg/mg  lysate. USP3 was also immunoprecipitated by rabbit anti-USP3  antibody NB100-77284, which recognizes a downstream epitope.  Detection: Chemiluminescence  with an exposure time of 10 seconds. Rabbit IgG Aff - Purified Hu IP, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

USP3

USP3 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

USP3

USP3 Human Purified
2 µg / €300.00
  Abnova Taiwan Corp.

USP3

USP3 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

USP3

USP3 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

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