USP5 / ISOT antibody

Principal name

USP5 / ISOT antibody

Alternative names for USP5 / ISOT antibody

Ubiquitin carboxyl-terminal hydrolase 5, Isopeptidase T, Ubiquitin thioesterase 5, Ubiquitin-specific-processing protease 5, Deubiquitinating enzyme 5

SwissProt ID

P45974 (Human), P56399 (Mouse)

Gene ID

8078 (USP5)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Mky (Monkey)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Flow Cytometry (F), Paraffin Sections (P), Frozen Sections (C)

Background of USP5 / ISOT antibody

Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

USP5 / ISOT antibody

Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-USP5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501287, Dilution 1:50) Mouse IgG2a OTI1F8 Purified Can, Hu, Mky, Rt P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

USP5 / ISOT antibody

Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-USP5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501288, Dilution 1:50) Mouse IgG1 OTI2F5 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

USP5 / ISOT antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-USP5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501289, Dilution 1:50) Mouse IgG2a OTI2H5 Purified Can, Hu, Mky, Rt P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

USP5 / ISOT antibody

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-USP5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501290, Dilution 1:50) Mouse IgG2a OTI2E3 Purified Can, Hu, Mky, Rt P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

USP5 / ISOT antibody

Immunohistochemistry-Paraffin: USP5 Antibody [NB100-77286] - FFPE section of human ovarian carcinoma. Affinity purified rabbit anti-USP5used at a dilution of 1:500. Detection: DAB staining using Immunohistochemistry Accessory Kit Rabbit IgG Aff - Purified Hu F, ICC/IF, IP, P
0.1 mg / €440.00
  Novus Biologicals Inc.

USP5 / ISOT antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY USP5 (RC202624, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-USP5. Mouse IgG1 OTI1F9 Purified Hu, Mky WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

USP5 / ISOT antibody

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-USP5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501312, Dilution 1:50) Mouse IgG1 OTI1A8 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

USP5 / ISOT (C-term) antibody

Western blot analysis of anti-USP5 Antibody (C-term)(Cat.#AP12012PU-N) to detect USP5 in HL-60 cell lysate. Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

USP5 / ISOT antibody

USP5 polyclonal antibody (A01), Lot # 051108JC01 Western Blot analysis of USP5 expression in SJCRH30 ( Cat # L027V1 ). Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

USP5 / ISOT antibody

Detection limit for recombinant GST tagged USP5 is approximately 0.03ng/ml as a capture antibody. Mouse IgG2a 2C8 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

USP5 / ISOT antibody

USP5 / ISOT Mouse IgG2a 4G4 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

USP5 / ISOT antibody

The USP5 polyclonal antibody ( Cat # PAB4182 ) is used in Western blot to detect USP5 in HL-60 cell lysate . Rabbit Hu E, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

USP5 / ISOT antibody

Western Blot and Immunoprecipitation: USP5 Antibody [NB100-77285] - Detection of Human and Mouse USP5/IsoT on HeLa whole cell lysate using NB100-77285. USP5/IsoT was also immunoprecipitated by rabbit anti-USP5/IsoT antibody NB100-77286. Rabbit IgG Aff - Purified Hu, Ms IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

USP5 / ISOT antibody

Western Blot: USP5 Antibody [NBP1-32590] - (30 ug whole cell lysate) A:MOLT4  B:Raji  7.5% SDS PAGE diluted at 1:1000 Rabbit IgG Hu P, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

USP5 / ISOT

USP5 / ISOT Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

USP5 / ISOT

USP5 / ISOT Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

USP5 / ISOT

USP5 / ISOT Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

USP5 / ISOT

USP5 / ISOT Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

USP5 / ISOT

USP5 / ISOT Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates (Overexpressed)

Catalog No.    

USP5 overexpression lysate

USP5 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

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