USP8 / UBPY antibody

Principal name

USP8 / UBPY antibody

Alternative names for USP8 / UBPY antibody

Ubiquitin carboxyl-terminal hydrolase 8, KIAA0055, Ubiquitin thioesterase 8, Ubiquitin-specific-processing protease 8, Deubiquitinating enzyme 8

SwissProt ID

P40818 (Human), Q80U87 (Mouse)

Gene ID

9101 (USP8), 9100 (USP10), 910133 (pgl)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Ms (Mouse), Por (Porcine), Rb (Rabbit), Rt (Rat), Ze (Zebrafish)

Available hosts

Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), PLA/Proximity ligation assay (PLA)

Background of USP8 / UBPY antibody

Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

USP8 / UBPY antibody

WB Suggested Anti-USP8 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: 293T cell lysate USP8 is supported by BioGPS gene expression data to be expressed in HEK293T Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

USP8 / UBPY antibody

WB Suggested Anti-USP8 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: HCT15 cell lysate USP8 is supported by BioGPS gene expression data to be expressed in HCT15 Rabbit IgG Purified Bov, Can, Eq, Hu, Por, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

USP8 / UBPY (N-term) antibody

Western blot analysis using anti-USP8 (N-term) Pab (Cat.#AP12017PU-N) to detect USP8 in transfected 293T cells. Data is kindly provided by Lily Yen from University of California Davis (Davis, CA). Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

USP8 / UBPY (C-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

USP8 / UBPY (C-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

USP8 / UBPY antibody

The USP8 polyclonal antibody ( Cat # PAB4187 ) is used in Western blot to detect USP8 in transfected 293T cells . Data is kindly provided by Lily Yen from University of California Davis ( Davis, CA ) . Rabbit Hu E, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

USP8 / UBPY antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with USP8 polyclonal antibody ( Cat # PAB4188 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . BC = breast carcinoma . Rabbit Hu E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

USP8 / UBPY (C-term) antibody

USP8 / UBPY Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

USP8 / UBPY (N-term) antibody

USP8 / UBPY Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

USP8 / UBPY antibody

Western Blot: UBPY/USP8 Antibody [NBP1-50001] - Detection of Human USP8 by Western Blot and Immunoprecipitation.  Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells.  Antibodies: Affinity purified rabbit anti-USP8 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate.  USP8 was also immunoprecipitated by rabbit anti-USP8 antibody which recognizes a downstream epitope.  For blotting immunoprecipitated USP8, the ReliaBLOT®  Reagents and Procedures were used.   Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B). Rabbit Aff - Purified Hu IP, PLA, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

USP8 / UBPY antibody

Immunohistochemistry-Paraffin: UBPY/USP8 Antibody [NBP1-89643] - Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

USP8 / UBPY (transcript variant 1)

USP8 / UBPY Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

USP8 / UBPY (transcript variant 2)

USP8 / UBPY Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

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