USP9X antibody

Principal name

USP9X antibody

Alternative names for USP9X antibody

USP9, FAM, Probable ubiquitin carboxyl-terminal hydrolase FAF-X, DFFRX, Ubiquitin thioesterase FAF-X, Fat facets protein-related, X-linked

SwissProt ID

P70398 (Mouse), Q93008 (Human)

Gene ID

8239 (USP9X)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Mky (Monkey), Bov (Bovine), Eq (Equine), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Paraffin Sections (P)

Background of USP9X antibody

USP9X is an ubiquitin-specific protease involved in the removal and recycling of ubiquitin from proteins degraded in proteasomes. As a deubiquitinating enzyme, USP9x has a critical function in tight junction biogenesis by controlling the levels of the exchange factor for Arf6 (EFA6), a protein shown to facilitate tight junction formation during a narrow temporal window preceding the establishment of cell polarity (1). USP9X is also located on the X-chromosome but escapes X-inactivation, and defects in USP9X may cause gonadal degeneration in Turner syndrome.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

USP9X antibody

Western Blot: USP9x Antibody - Western blot analysis of USP9X in NIH/3T3 whole cell lysate using anti-USP9X. Rabbit Purified Hu, Ms, Rt C, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

USP9X antibody

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-USP9X mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800088) Mouse IgG2a OTI2G7 Purified Can, Hu, Mky, Ms, Rt P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

USP9X antibody

Sample Type: 1. total HeLa cell extract (100ug) 2. total HeLa cell extract incubated with HA-UbVME (100ug) 3. Rat Liver cytosolic extract (100ug) 4. Rat Liver cytosolic extract incubated with HA-UbVME (100ug) Primary Dilution: 1:1000 Secondary Antibody: a Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rb, Rt P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

USP9X antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY USP9X (RC217531, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-USP9X. Mouse IgG1 OTI1D7 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

USP9X antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY USP9X (RC217531, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-USP9X. Mouse IgG1 OTI2B4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

USP9X (2479-2492) antibody

Brain, cortex, Human: Formalin-Fixed, Paraffin-Embedded (FFPE) Goat Aff - Purified Elephant, Eq, Hu, Mky, Ms, Prim, Rb P
50 µg / €470.00
  Acris Antibodies GmbH

USP9X antibody

USP9X Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

USP9X antibody

Immunofluorescence of monoclonal antibody to USP9X on HeLa cell. [antibody concentration 10 ug/ml] Mouse IgG1 1C4 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

USP9X antibody

Detection limit for recombinant GST tagged USP9X is approximately 0.1ng/ml as a capture antibody. Mouse IgG1 1A7 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

USP9X antibody

Detection limit for recombinant GST tagged USP9X is approximately 0.03ng/ml as a capture antibody. Mouse IgG1 4B7 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

USP9X antibody

Detection limit for recombinant GST tagged USP9X is approximately 0.03ng/ml as a capture antibody. Mouse IgG1 2A8 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

USP9X antibody

USP9X monoclonal antibody (M05), clone 5D7. Western Blot analysis of USP9X expression in Jurkat(Cat # L017V1 ). Mouse IgG1 5D7 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

USP9X antibody

USP9X monoclonal antibody (M05), clone 5D7. Western Blot analysis of USP9X expression in human placenta. Mouse IgG1 5D7 Ascites Hu E, WB
0.2 ml / €370.00
  Abnova Taiwan Corp.

USP9X antibody

Detection limit for recombinant GST tagged USP9X is approximately 0.3ng/ml as a capture antibody. Mouse IgG1 3E12 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

USP9X antibody

Detection limit for recombinant GST tagged USP9X is approximately 0.03ng/ml as a capture antibody. Mouse IgG1 5H4 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

USP9X antibody

USP9X Mouse IgG1 2A9 Purified Hu E, WB
50 µg / €370.00
  Abnova Taiwan Corp.

USP9X antibody

USP9X Mouse IgG1 2A9 Hu E, WB
0.2 ml / €370.00
  Abnova Taiwan Corp.

USP9X (C-term) antibody

USP9X Rabbit Aff - Purified Hu P, WB
50 µg / €350.00
  AVIVA Systems Biology

USP9X antibody

Western Blot: USP9X Antibody [NB100-61591] - Detection of Human and Mouse USP9X by Western Blot  (h&m) and Immunoprecipitation (h). Samples: Whole cell  lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of  IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg)  cells. Antibodies: Affinity purified rabbit anti-USP9X antibody  NB100-61591 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B)  and used for IP at 3 mcg/mg lysate. USP9X was also  immunoprecipitated by rabbit anti-USP9X antibody NB100-61590,  which recognizes an upstream epitope.  Detection:  Chemiluminescence with exposure times of 1 second (A and B). Rabbit IgG Aff - Purified Hu, Ms IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

USP9X antibody

USP9X Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, WB
0.1 ml / €360.00
  Novus Biologicals Inc.

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