VAV2 antibody

Principal name

VAV2 antibody

Alternative names for VAV2 antibody

VAV-2, Guanine nucleotide exchange factor VAV2

SwissProt ID

P52735 (Human), Q60992 (Mouse)

Gene ID

7410 (VAV2)

Available reactivities

Hu (Human), Can (Canine), Ms (Mouse), Por (Porcine), Rt (Rat)

Available hosts

Sheep, Goat, Rabbit

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of VAV2 antibody

The immunogen used for the anti-human Vav-2 SHD region was a his tagged fusion protein Vav-2 corresponding to amino acid sequence 578 - 878 and having a molecular weight of 100kD. Vav-2 has a molecular weight of 100 kD and shares over a 50% homology at the amino acid and nucleic acid level with Vav. Vav-2 is a ubiquitously expressed structural homolog of the Vav protooncogene that is expressed preferentially in hematopoetic cells. Both proteins are comprised of a Dbl- homology (DH) domain with guanosine nucleotide exchange (GEF) activity exclusively directed towards Rho/Rac GTPases, a pleckstrin homology (PH) domain, a calponin-homology (CH) region, an acidic domain (AD) a zinc finger butterfly motif, two SH3 regions and one SH2 domain. GEF activity of RhoA family G proteins is induced by tyrosine phosphorylation in wild type vav-2, and is constitutively activated in N terminus deleted oncogene forms. Constitutive expression of a Vav-2 oncoprotein may result in morphological alterations including highly enlarged cells in which karyokinesis and cytokinesis frequently are uncoupled.

General readings

1. Henske EP, Short MP, Jozwiak S, Bovey CM, Ramlakhan S, Haines JL, Kwiatkowski DJ. Identification of VAV2 on 9q34 and its exclusion as the tuberous sclerosis gene TSC1.
Ann Hum Genet. 1995 Jan;59 ( Pt 1):25-37. PMID: 7762982

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

VAV2 antibody

TA302768 staining (1.5µg/ml) of 293 lysate (RIPA buffer, 30µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt E, WB
20 µg / €200.00
  Acris Antibodies GmbH

VAV2 antibody

TA302768 staining (1.5µg/ml) of 293 lysate (RIPA buffer, 30µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

VAV2 (SHD Region) antibody

HEK293 cells were transfected with either emply expression vector (pSRa) or pSRa-Vav2 driving expression of human Vav2. Indicated antibodies (SHD or DPH) were used to immunoprecipitate Vav2 from 300ug protein lysates. Immunoprecipitated activity was identified by immunoblot of complexes using either DPH (upper gel) or SHD (lower gel) antisera at (1:10000). Immunoprecipitation of Endogenous/Exogenous Vav2 from HEK 283 (75 µg lysate per lane). Top gel - i.p. Ab: DPH or SHD preimmune serum (2.5 µl/300 µg lysate). DPH or SHD alpha Vav2 antiserum (2.5 µl/300 µg lysate). Western Blot Ab: DPH anti Vav2 1:10000. Bottom Gel - i.p. Ab DPH or SHD preimmune sera (2.5 µl/300 µg lysate). DPH or SHD anti Vav2 antisera (2.5 µl/300 µg lysate). Wersterm blot Ab - SHD anti Vav2 antibody 1:10000. Sheep IgG Purified Hu IP, WB
0.25 mg / €360.00
  Acris Antibodies GmbH

VAV2 (DPH Region) antibody

HEK293 cells were transfected with either emply expression vector (pSR_) or pSRa-Vav2 driving expression of human Vav2. Indicated antibodies (SHD or DPH) were used to immunoprecipitate Vav2 from 300µg protein lysates.  Immunoprecipitated activity was identified by immunoblot of complexes using either DPH (upper gel) or SHD (lower gel) antisera at (1:10000). Immunoprecipitation of Endogenous/Exogenous Vav2 from HEK 283 (75 µg lysate per lane). Top gel - i.p. Ab: DPH or SHD preimmune serum (2.5 µl/300µg lysate). DPH or SHD alpha Vav2 antiserum (2.5 µl/300µg lysate). Western Blot Ab: DPH anti Vav2 1:10000. Bottom Gel - i.p. Ab DPH or SHD preimmune sera (2.5 µl/300µg lysate). DPH or SHD anti Vav2 antisera (2.5µl/300ug lysate). Wersterm blot Ab - SHD anti Vav2 antibody 1:10000. Sheep IgG Purified Hu IP, WB
0.25 mg / €360.00
  Acris Antibodies GmbH

VAV2 antibody

Western blot (WB) analysis of VAV2 antibody (Cat.-No.: AP20506PU-N) in extracts from THP1 cells. Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

VAV2 antibody

VAV2 polyclonal antibody ( Cat # PAB6123 ) staining ( 1.5 µg/ml ) of 293 lysate ( RIPA buffer, 30 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

VAV2 antibody

Immunoprecipation results using western blot analysis. 293 cells were transfected with pSRa-VAV2 driving expression of human VAV2. DPH antiserum ( lane 1 ) or DPH-VAV2 antibody ( lane 2 ) were used to immunoprecipitate VAV2 from 300µgprotein lysates. Each lane was loaded with 75 µg/ml the immunoprecipitated proteins. Sheep Hu IP
0.25 mg / €400.00
  Abnova Taiwan Corp.

VAV2 antibody

Immunoprecipation results using Western blot analysis. 293 cells were transfected with pSRa-VAV2 driving expression of human VAV2. SHD antiserum ( lane 1 ) or SHD-VAV2 antibody ( lane 2 ) were used to immunoprecipitate VAV2 from 300ug protein lysates. Each lane was loaded with 75 µg/ml the immunoprecipitated proteins. Sheep Hu IP
0.25 mg / €400.00
  Abnova Taiwan Corp.

VAV2 pTyr142 antibody

Immunohistochemistry analysis of paraffin-embedded human brain, using VAV2 (Phospho-Tyr142) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, ICC/IF, P, WB
50 µg / €205.00
  Assay Biotech

VAV2 pTyr142 antibody

Immunohistochemistry analysis of paraffin-embedded human brain, using VAV2 (Phospho-Tyr142) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, ICC/IF, P, WB
0.1 mg / €310.00
  Assay Biotech

VAV2 antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using VAV2 (Ab-142) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, ICC/IF, P, WB
50 µg / €205.00
  Assay Biotech

VAV2 antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using VAV2 (Ab-142) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, ICC/IF, P, WB
0.1 mg / €310.00
  Assay Biotech

VAV2 antibody

Immunofluorescent analysis of VAV2 staining in THP1 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4  C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Hu ICC/IF, IP, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

VAV2 antibody

Immunofluorescent analysis of VAV2 staining in THP1 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4  C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Hu ICC/IF, IP, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

VAV2 antibody

Immunofluorescent analysis of VAV2 staining in THP1 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4  C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Hu ICC/IF, IP, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

VAV2 antibody

NB 100-895 staining (1.5µg/ml) of 293 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat IgG Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

VAV2 antibody

Immunohistochemistry: VAV2 Antibody (EP1067Y) [NB110-57640] - Immunohistochemical staining of paraffin-embedded human cervical carcinoma using anti-Vav2 (cat. #110-57640). Rabbit IgG EP1067Y Ascites Hu, Ms, Rt ICC/IF, IP, P, WB
0.1 ml / €490.00
  Novus Biologicals Inc.

VAV2 antibody

VAV2 Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, P, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

VAV2 antibody

VAV2 Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, P, WB
50 µg / €290.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

VAV2 (transcript variant 2)

VAV2 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

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