VDAC1 antibody

Principal name

VDAC1 antibody

Alternative names for VDAC1 antibody

VDAC, Porin 31HL, Porin 31HM, Mitochondria marker, Voltage-dependent anion-selective channel protein 1, Outer mitochondrial membrane protein porin 1, Plasmalemmal porin

SwissProt ID

P21796 (Human), P45879 (Bovin), Q60932 (Mouse), Q9MZ16 (Pig), Q9TT15 (Rabit), Q9Z2L0 (Rat)

Gene ID

7416 (VDAC1), 83529 (Vdac1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Por (Porcine), Rb (Rabbit), Av (Birds), Fi (Fish), Mam (Mammalia), Re (Reptiles), Can (Canine), Chk (Chicken), Ze (Zebrafish), African clawed frog, Chimp (Chimpanzee), Eq (Equine), Macaque, Mky (Monkey)

Available hosts

Rabbit, Mouse

Available applications

Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunoprecipitation (IP), Dot blot (Dot), Flow Cytometry (F), Frozen Sections (C)

Background of VDAC1 antibody

Voltage-dependent anion channel (VDAC) is an outer mitochondrial membrane channel protein expressed in a ubiquitous manner as it is required for proper mitochondrial function. The three isoforms expressed in higher eukaryotes are highly conserved. VDAC1, VDAC2 and VDAC3 allow the entry and exit of most metabolites in and out of the mitochondria. The channel spans the membrane 19 times through antiparallel b strands. Studies show that VDAC channels exhibit ion selectivity and are dependent on voltage. When in the open state, VDAC channels conduct mainly organic anions such as ATP, ADP and inorganic phosphate (Pi). In the closed state they still conduct ions, but much smaller and mostly inorganic like K+, + and Ca2+ . However, while cells are metabolically active, VDAC channels are constitutively open in order to eble oxidative phosphorylation in the mitochondria to take place (through the passage of ADP across the membrane). VDAC channels are best described for their role in apoptosis. In the early stages of the sigling pathway, a proapoptotic factor, a member of the Bcl2 family is responsible for closing the channel thereby decreasing the release of ADP and ATP from the mitochondria. VDAC channels are also implicated in the Warburg effect, which is typical to cancer cell. In such cells, hexokise (the first enzyme in the glycolytic pathway responsible for phosphorylating glucose on C6 to yield glucose-6 phosphate) known to be up-regulated, binds to VDAC1 and takes up most of the ATP released by the channel in order to phosphorylate glucose. This action speeds up glycolysis if downstream reactions function properly. If however a glycolysis step is inhibited or down-regulated, glucose-6-phosphate builds up and consequently inhibits hexokise, thereby restoring proper mitochondrial function. VDAC is also detected in the plasma membrane of various cell types including lymphocytes, epithelial cells, and astrocytes. The postulated roles for VDAC in the plasma membrane mainly involve the regulation of cellular ATP release and volume control. VDAC have also been proposed to function as the plasma membrane maxi anion channels. None of the isoforms are essential for viability. Knock out of VDAC3 in mice causes sterility in males. That of VDAC1 and VDAC2 affects breathing and the individual knock out of VDAC1 causes embryonic death in mice in some cases.

General readings

1. Blachly-Dyson, E., Zambronicz, E. B., Yu, W. H., Adams, V., McCabe, E. R. B., Adelman, J.,
Colombini, M., Forte, M.Cloning and functional expression in yeast of two human isoforms of the
outer mitochondrial membrane channel, the voltage-dependent anion channel.J. Biol. Chem. 268:
1835-1841, 1993.
2. Geisler, S., Holmstrom, K. M., Skujat, D., Fiesel, F. C., Rothfuss, O. C., Kahle, P. J., Springer,
W.PINK1/Parkin-mediated mitophagy is dependent on VDAC1 and p62/SQSTM1.Nature Cell Biol.
12: 119-131, 2010.
3. Huizing, M., Ruitenbeek, W., van den Heuvel, L. P., Dolce, V., Iacobazzi, V., Smeitink, J. A. M.,
Palmieri, F., Trijbels, J. M. F.Human mitochondrial transmembrane metabolite carriers: tissue
distribution and its implication for mitochondrial disorders.J. Bioenerg. Biomembr. 30: 277-284,
1998.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

VDAC1 antibody

Sample (30 ug of whole cell lysate). A: A431 . 12% SDS PAGE. VDAC1 antibody. TA308959 diluted at 1:1000. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

VDAC1 antibody

Adult rat hippocampus immunohistochemistry Mouse IgG2a N152B/23 Supernatant Hu, Ms, Rt C, ICC/IF, WB
5 ml / €320.00
  Antibodies Incorporated

NeuroMab

VDAC1 antibody

Adult rat hippocampus immunohistochemistry Mouse IgG2a N152B/23 Purified Hu, Ms, Rt C, ICC/IF, WB
0.1 ml / €380.00
  Antibodies Incorporated

NeuroMab

VDAC1 (185-197) (Loading Control) antibody

Western Blot of Rabbit Anti-VDAC/Porin Antibody. Lane 1: rat heart whole cell lysate. Lane 2: none. Load: 35 µg per lane. Primary antibody: VDAC/Porin antibody at 1:1,200 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~32 kDa corresponding to VDAC/Porin (arrowhead). Other band(s): none. Rabbit IgG Aff - Purified Av, Hu, Mam, Re E, WB
0.2 mg / €325.00
  OriGene Technologies, Inc.

VDAC1 antibody

Predicted band size: 30 kDa . Positive control: Hela cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution Exposure time: 5 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

VDAC1 antibody

Western blot analysis of extracts of various cell lines, using VDAC1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

VDAC1 antibody

Western blot analysis of rat kidney (lanes 1 and 4), brain (lanes 2 and 5) mouse brain (lanes 3 and 6) membrane and CCF-SSTGI (lanes 7 and 8) cell line lysate: 1-3, 7. Anti-Pan Voltage Dependent Anion Channels antibody, (1:200). 4-6, 8. Anti-Pan Voltage Dependent Anion Channels antibody, preincubated with the control peptide antigen. Rabbit Purified Ms, Rt WB
0.2 ml / €435.00
  OriGene Technologies, Inc.

VDAC1 antibody

Human Brain Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2a 2A8 Purified Hu E, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

VDAC1 (3594-100) antibody

Western blot analysis of VDAC/Porin with 3T3 cell lysate. Rabbit Purified Bov, Hu, Ms, Por, Rb, Rt C, WB
0.1 mg / €390.00
  Acris Antibodies GmbH

VDAC1 antibody

Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Liver. Rabbit Aff - Purified Hu E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

VDAC1 (N-term) antibody

Western blot analysis of VDAC1 Antibody (N-term) (AP17825PU-N) in A375 cell line and mouse heart tissue lysates (35ug/lane). VDAC1 (arrow) was detected using the purified Pab. Rabbit Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

VDAC1 antibody

Detection limit for recombinant GST tagged VDAC1 is approximately 1ng/ml as a capture antibody. Mouse IgG2a 4C4 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

VDAC1 antibody

VDAC1 Mouse IgG2a 2E10 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

VDAC1 antibody

VDAC1 Mouse IgG2a 2A8 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

VDAC1 antibody

VDAC1 Mouse IgG2a 2A8 Ascites Hu E, WB
0.2 ml / €370.00
  Abnova Taiwan Corp.

VDAC1 antibody

Immunoblot analysis of cell lysates. Lane 1 : Hela cell lysate and lane 2 : HepG2 cell lysate. ( * N.S : Nonspecific band ) Rabbit Hu, Ms, Rt WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

VDAC1 antibody

Western blot analysis of VDAC1 expression in NIH/3T3 cell lysate. Rabbit Bov, Hu, Ms, Rb, Rt WB
0.1 mg / €470.00
  Abnova Taiwan Corp.

VDAC1 antibody

Western blot using VDAC1 polyclonal antibody ( Cat # PAB11290 ) detects a single band at ~32 kDa corresponding to VDAC1 ( arrowhead ) present in a rat heart tissue lysate.
Approximately 35 µg of lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose.
After blocking, the membrane was probed with the primary antibody diluted to 1 : 1,200 at 4°C overnight.
Washes were followed by reaction with a 1 : 10,000 dilution of IRDyeTM800 conjugated Gt-a-Rabbit IgG [H&L] MXHu for 45 min at room temperature.
IRDyeTM800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
IRDye is a trademark of LI-COR, Inc. Rabbit Av, Hu E, WB
0.2 mg / €470.00
  Abnova Taiwan Corp.

VDAC1 antibody

Human Liver; Rabbit Anti-VDAC1 Antibody. Catalog Number: ARP35122. Paraffin Embedded Tissue: Human Liver. Cellular Data: Hepatocytes. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X; VDAC1 antibody - C-terminal region (ARP35122_T100) in Human Liver cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Por, Rb, Rt, Ze P, WB
0.1 mg / €310.00
  AVIVA Systems Biology

VDAC1 antibody

VDAC1 Rabbit IgG Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €290.00
  Boster Immunoleader

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