VEGF-A antibody

Principal name

VEGF-A antibody

Alternative names for VEGF-A antibody

VEGFA, VEGF, VPF, Vascular endothelial growth factor A, Vascular permeability factor

SwissProt ID

P15691 (Bovin), P15692 (Human), P16612 (Rat), P26617 (Cavpo), P49151 (Pig), P50412 (Sheep), P67964 (Chick), Q00731 (Mouse), Q99PS1 (Mesau), Q9GKR0 (Horse), Q9MYV3 (Canfa)

Gene ID

7422 (VEGFA), 403802, 22339 (Vegfa), 83785, 281572, 6967422 (aaeB)

Available reactivities

Hu (Human), Bov (Bovine), Por (Porcine), Sh (Sheep), Can (Canine), Ms (Mouse), Rt (Rat), Rb (Rabbit), Chk (Chicken), Eq (Equine), GP (Guinea Pig), Mky (Monkey), Macaque, Hst (Hamster)

Available hosts

Mouse, Rabbit, Goat, Rat, Chicken

Available applications

Paraffin Sections (P), Frozen Sections (C), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Functional assay (FN), Radioimmunoassay (R), Neutralisation (NEUT), ELISA (detection) (E(detection)), Flow Cytometry (F)

Background of VEGF-A antibody

Vascular endothelial growth factor (VEGF) is a member of the cysteine-knot growth factor superfamily. Five VEGF splice variants exist including VEGF-121, VEGF-145, VEGF-165, VEGF-189; and VEGF-206. VEGF-165 is the most abundant and active isoform. VEGF-165 functions as a growth factor in angiogenesis, vasculogenesis and endothelial cell growth. It is widely expressed by normal tissues and by many tumor cells in response to a variety of cytokines, oncogene expression and hypoxia. VEGF-165 acts as a specific mitogen and survival factor for vascular endothelial cells, inducing microvascular permeability, cell migration and regulates the differentiation and survival of hematopoietic progenitor cells to affect hematopoiesis, immune function and tumour progression. VEGF-165 also plays roles in neurogenesis and blood brain barrier function. Local administration of VEGF-165 has been shown to enhance re-endothelialization in denuded arteries in vivo and increase new blood vessel formation in ischemic myocardium and limbs. Inhibitors of VEGF also have potential therapeutic applications, particularly as angiogenesis inhibitors to inhibit the growth of solid tumours. Structurally, VEGF-165 is related to platelet-derived growth factor (PDGF) and exists as disulfide linked homodimer and contains 4 potential N-linked glycosylation sites. This is a HCX protein. HCX Expression System Details HCX proteins mimic the proteins in the human body because they are expressed from human, rather than animal, insect or bacterial cells. This process gives them human post-translational modifications. Recombinant DNA techniques allow a human protein with the correct amino acid sequence to be expressed in a non-human cell line. However, non-human cells lack the appropriate cellular machinery, such as specific glycosyltransferases, necessary to produce the correct human post-translational modifications of a protein. An extreme example is seen in E. coli cells, which produce recombinant proteins with no glycosylation, as the above figure illustrates. Rodent and yeast cells are able to glycosylate proteins, but they are still different from glycosylation in human cells. Expression System Resultant Proteins Human (e.g. K562, HEK293) Correct amino acid sequence Human post-translational modifications Rodent (e.g. CHO, NSO) Correct amino acid sequence Some natural glycosylation - not human-like Yeast (e.g. Pichia) Correct amino acid sequence Some natural glycosylation - not human-like E.Coli Correct amino acid sequence No PTMs Although there have been significant attempts to make non-human cell derived cytokines more human-like, there is a growing awareness that in many instances, particularly in therapeutics, cytokines should mimic those found in the body as closely as possible.

General readings

Yasuji Ueda., et al. (2004) Cancer Chemother Pharmacol. 54(1):16-24. Neufeld G., et al. (1999) FASEB J. 13(1):9-22.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

VEGF165B (C-term) antibody

VEGF165B Mouse IgG1 7F17 Purified Hu C, P, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

VEGF-A antibody

Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-VEGF mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500042) Mouse OTI4G3 Purified Can, Hu P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

VEGF-A antibody

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-VEGF mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500289) Mouse IgG1 OTI4E3 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

VEGF-A antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-VEGF mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500288, Dilution 1:50) Mouse IgG2b OTI2F7 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

VEGF-A (Isoform 165) antibody

Immunohistochemistry with a paraffin-bedded section of human Glioblastoma tissue. Mouse IgG1 7G7 Purified Hu E, ICC/IF, P, WB
0.1 mg / €330.00
  Acris Antibodies GmbH

VEGF-A (C-term) antibody

LNCap; . Sample Type :. LNCap cells . Primary Antibody Dilution :. 1:500. Secondary Antibody :. Anti-rabbit-HRP . Secondary Antibody Dilution :. 1:5000. Color/Signal Descriptions :. Brown: VEGFA Blue: DAPI . Gene Name :. VEGFA. Submitted by :. Christina Theodorpoulos, Queensland Univ. of Technology; VEGFA antibody - C-terminal region (ARP60981_P050) in LNCap cells using Immunohistochemistry Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

VEGF-A (N-term) antibody

Human angiosarcoma stained with anti-VEGF antibody Rabbit Purified Hu IP, P, WB
0.5 ml / €360.00
  Acris Antibodies GmbH

VEGF-A (N-term) antibody

Human angiosarcoma stained with anti-VEGF antibody Rabbit Aff - Purified Hu IP, P, WB
1 ml / €490.00
  Acris Antibodies GmbH

VEGF-A (N-term) antibody

Human angiosarcoma stained with anti-VEGF antibody Rabbit Purified Hu IP, P, WB
0.1 ml / €190.00
  Acris Antibodies GmbH

VEGF-A antibody

Detection of VEGFA doublet in CSF-IR/VEGFA chimera transfected lysate. ECL 10 second exposure. Rabbit IgG Purified Bov, Can, Chk, Eq, Hst, Hu, Ms, Por, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

VEGF-A (N-term) antibody

Western blot analysis of VEGF1 Antibody (N-term) polyclonal antibody(Cat.#TA302292)(arrow). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the VEGF1 gene (Lane 2) (Origene Technologies).  Molecular weight of monomeric VEGF1 is 27042 Da; higher observed molecular weight band is consistent with common form of disulfide linked homodimer. Rabbit Ig Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

VEGF-A antibody

(0.1ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Purified Can, Hu, Por WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

VEGF-A antibody

Predicted band size: 27 kDa . Positive control: Human cervical cancer tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution Exposure time: 10seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

VEGF-A antibody

Predicted band size: 35 kDa . Positive control: 293T and 231 cell lysate. Recommended dilution: 1/500-2000. (Gel: 12%SDS-PAGE Lane1: 293T cell lysate Lane2: 231 cell lysate Lysates: 40 ug per lane Primary antibody: 1/400 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 45 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

VEGF-A antibody

Predicted band size: 35 kDa . Positive control: Hela, Jurkat, 293T and 231 cell lysate. Recommended dilution: 1/500-2000. (Gel: 12%SDS-PAGE Lane1: Hela, cell lysate Lane2: Jurkatcell lysate Lane3: 293T cell lysate Lane4: 231 cell lysate Lysates: 40 ug per lane Primary antibody: 1/450 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 minutes) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

VEGF-A antibody

IHC of human breast invasive ductal carcinoma.  The recommended concentrations are 5.0 ug/ml-15.0 ug/ml with a 30-minute incubation at room temperature or an overnight incubation at 4C.  An HRP-labeled polymer detection system was used with a DAB chromogen. This antibody was also tested in an HRP-biotin labeling detection system. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is recommended.  Optimal concentrations and conditions may vary. Goat IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

VEGF-A antibody

This antibody stained formalin-fixed, paraffin-embedded sections of Human breast invasive ductal carcinoma.  The recommended concentrations are 2.5 - 5.5 mg/ml with an overnight incubation at 4C.  An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is recommended.  Optimal concentrations and conditions may vary. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

VEGF-A antibody

To detect mVEGF by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant mVEGF. Rabbit IgG Purified E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

VEGF-A antibody

To detect mVEGF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine VEGF (TA328560) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mVEGF. Rabbit IgG Purified E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

VEGF-A antibody

To detect Rat VEGF by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat VEGF. Rabbit IgG Purified Rt E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

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