VEGF-A antibody

Principal name

VEGF-A antibody

Alternative names for VEGF-A antibody

VEGFA, VEGF, VPF, Vascular endothelial growth factor A, Vascular permeability factor

SwissProt ID

P15691 (Bovin), P15692 (Human), P16612 (Rat), P26617 (Cavpo), P49151 (Pig), P50412 (Sheep), P67964 (Chick), Q00731 (Mouse), Q99PS1 (Mesau), Q9GKR0 (Horse), Q9MYV3 (Canfa)

Gene ID

7422 (VEGFA), 403802, 22339 (Vegfa), 83785, 281572

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Por (Porcine), Chk (Chicken), GP (Guinea Pig), Sh (Sheep), Macaque, Can (Canine), Mky (Monkey), Eq (Equine), Hst (Hamster)

Available hosts

Mouse, Rabbit, Goat, Chicken

Available applications

Paraffin Sections (P), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Functional assay (FN), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Frozen Sections (C), Radioimmunoassay (R), ELISA (detection) (E(detection))

Background of VEGF-A antibody

Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and in adults . It is a member of the PDGF family that is characterized by the presence of eight conserved cysteine residues in a cystine knot structure and formation of anti-parallel disulfide-linked dimers . Alternately spliced isoforms of 120, 164, and 188 amino acids (aa) have been found in rats and mice, while 121, 145, 165, 183, 189, and 206 aa isoforms have been identified in humans . In humans, VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189 . The same pattern may exist in rats and mice. Isoforms other than VEGF120 and VEGF121 contain basic heparin-binding regions and are not freely diffusible . Rat VEGF164 shares 97% aa sequence identity with corresponding regions of mouse, 88% with human and bovine, 89% with porcine and canine, and 90% with feline and equine VEGF. VEGF is expressed in multiple cells and tissues including skeletal and cardiac muscle , hepatocytes , osteoblasts , neutrophils , macrophages , keratinocytes , brown adipose tissue , CD34+ stem cells , endothelial cells , fibroblasts, and vascular smooth muscle cells . VEGF expression is induced by hypoxia and cytokines such as IL-1, IL-6, IL-8, Oncostatin M, and TNF-. . The isoforms are differentially expressed during development and in the adult .

VEGF dimers bind to two related receptor tyrosine kinases, VEGF R1 (also called Flt-1) and VEGF R2 (Flk-1/KDR) and induce their homodimerization and autophosphorylation . These receptors have seven extracellular immunoglobulin-like domains and an intracellular split tyrosine kinase domain. They are expressed on vascular endothelial cells and a range of non-endothelial cells. Although VEGF affinity is highest for binding to VEGF R1, VEGF R2 appears to be the primary mediator of VEGF angiogenic activity . VEGF165 also binds the semaphorin receptor, neuropilin-1, which promotes complex formation with VEGF R2 .

VEGF is best known for its role in vasculogenesis. During embryogenesis, VEGF regulates the proliferation, migration, and survival of endothelial cells , thus regulating blood vessel density and size but playing no role in determining vascular patterns. VEGF promotes bone formation through osteoblast and chondroblast recruitment and is also a monocyte chemoattractant . In postnatal life, VEGF maintains endothelial cell integrity and is a potent mitogen for micro- and macro-vascular endothelial cells. In adults, VEGF functions mainly in wound healing and the female reproductive cycle . In diseased tissues, VEGF promotes vascular permeability. It is thus thought to contribute to tumor metastasis by promoting both extravasation and tumor angiogenesis . Various strategies have been employed therapeutically to antagonize VEGF-mediated tumor angiogenesis . Circulating VEGF levels correlate with disease activity in autoimmune diseases such as rheumatoid arthritis, multiple sclerosis and systemic lupus erythematosus .

General readings

Conn, G. et al. (1990) J. Biol. Chem. 87:2628.

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Byrne, A.M. et al. (2005) J. Cell. Mol. Med. 9:777.

Robinson, C.J. and S.E. Stringer (2001) J. Cell. Sci. 114:853.

Richardson, R.S. et al. (1999) Am. J. Physiol. 277:H2247.

Sugishita, Y. et al. (2000) Biochem. Biophys. Res. Commun. 268:657.

Yamane, A. et al. (1994) Oncogene 9:2683.

Goad, D.L. et al. (1996) Endocrinology 137:2262.

Gaudry, M. et al. (1997) Blood 90:4153.

Mclaren, J. et al. (1996) J. Clin. Invest. 98:482.

Diaz, B.V. et al. (2000) J. Biol. Chem. 275:642.

Asano, A. et al. (1997) Biochem. J. 328:179.

Bautz, F. et al. (2000) Exp. Hematol. 28:700.

Namiki, A. et al. (1995) J. Biol. Chem. 270:31189.

Nauck, M. et al. (1997) Am. J. Respir. Cell. Mol. Biol. 16:398.

Angelo, L.S. and R. Kurzrock (2007) Clin. Cancer Res. 13:2825.

Neufeld, G. et al. (1999) FASEB. J. 13:9.

Kowalewski, M.P. et al. (2005) Accession #ABB82619.

Pan, Q. et al. (2007) J. Biol. Chem. 282:24049.

Weis, S.M. and D.A. Cheresh (2005) Nature 437:497.

Breier, G. (2000) Semin. Thromb. Hemost. 26:553.

Barleon, B. et al. (1996) Blood 87:3336.

Weis, S.M. and D.A. Cheresh (2005) Nature 437:497.

Thurston, G. (2002) J. Anat. 200:575.

Grothey, A. and E. Galanis (2009) Nat. Rev. Clin. Oncol. 6:507.

Carvalho, J.F. et al. (2007) J. Clin. Immunol. 27:246.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

VEGF165B (C-term) antibody

VEGF165B Mouse IgG1 7F17 Purified Hu C, P, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

VEGF-A antibody

  VEGFA (VEGF) mouse monoclonal antibody, clone OTI4G3 (formerly 4G3)  
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-VEGF mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500042) Mouse OTI4G3 Purified Can, Hu P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

VEGF-A antibody

  VEGFA (VEGF) mouse monoclonal antibody, clone OTI4E3 (formerly 4E3)  
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-VEGF mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500289) Mouse IgG1 OTI4E3 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

VEGF-A antibody

  anti-VEGFA (VEGF) mouse monoclonal antibody, clone OTI2F7 (formerly 2F7)  
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-VEGF mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500288, Dilution 1:50) Mouse IgG2b OTI2F7 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

VEGF-A (Isoform 165) antibody

Immunohistochemistry with a paraffin-bedded section of human Glioblastoma tissue. Mouse IgG1 7G7 Purified Hu E, ICC/IF, P, WB
0.1 mg / €330.00
  Acris Antibodies GmbH

VEGF-A antibody

  Rabbit Polyclonal Antibody against VEGFA  
Detection of VEGFA doublet in CSF-IR/VEGFA chimera transfected lysate. ECL 10 second exposure. Rabbit IgG Purified Bov, Can, Chk, Eq, Hst, Hu, Ms, Por, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

VEGF-A (N-term) antibody

  Rabbit Polyclonal Antibody against VEGFA (N-term)  
Western blot analysis of VEGF1 Antibody (N-term) polyclonal antibody(Cat.#TA302292)(arrow). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the VEGF1 gene (Lane 2) (Origene Technologies).  Molecular weight of monomeric VEGF1 is 27042 Da; higher observed molecular weight band is consistent with common form of disulfide linked homodimer. Rabbit Ig Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

VEGF-A antibody

  Goat Anti-VEGFA (aa288-299) Antibody  
(0.1ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Purified Can, Hu, Por WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

VEGF-A antibody

  Anti-VEGFA Rabbit Polyclonal Antibody  
Predicted band size: 27 kDa . Positive control: Human cervical cancer tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution Exposure time: 10seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

VEGF-A antibody

  Anti-VEGFA Rabbit Polyclonal Antibody  
Predicted band size: 35 kDa . Positive control: 293T and 231 cell lysate. Recommended dilution: 1/500-2000. (Gel: 12%SDS-PAGE Lane1: 293T cell lysate Lane2: 231 cell lysate Lysates: 40 ug per lane Primary antibody: 1/400 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 45 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

VEGF-A antibody

  Anti-VEGFA Rabbit Polyclonal Antibody  
Predicted band size: 35 kDa . Positive control: Hela, Jurkat, 293T and 231 cell lysate. Recommended dilution: 1/500-2000. (Gel: 12%SDS-PAGE Lane1: Hela, cell lysate Lane2: Jurkatcell lysate Lane3: 293T cell lysate Lane4: 231 cell lysate Lysates: 40 ug per lane Primary antibody: 1/450 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 minutes) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

VEGF-A antibody

  Anti-Human VEGF Goat Polyclonal Antibody  
IHC of human breast invasive ductal carcinoma.  The recommended concentrations are 5.0 ug/ml-15.0 ug/ml with a 30-minute incubation at room temperature or an overnight incubation at 4C.  An HRP-labeled polymer detection system was used with a DAB chromogen. This antibody was also tested in an HRP-biotin labeling detection system. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is recommended.  Optimal concentrations and conditions may vary. Goat IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

VEGF-A antibody

  Anti-Human VEGF Rabbit Polyclonal Antibody  
This antibody stained formalin-fixed, paraffin-embedded sections of Human breast invasive ductal carcinoma.  The recommended concentrations are 2.5 - 5.5 mg/ml with an overnight incubation at 4C.  An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is recommended.  Optimal concentrations and conditions may vary. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

VEGF-A antibody

  Biotinylated Anti-Murine VEGF Rabbit Polyclonal Antibody  
To detect mVEGF by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant mVEGF. Rabbit IgG Purified E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

VEGF-A antibody

  Anti-Murine VEGF Rabbit Polyclonal Antibody  
To detect mVEGF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine VEGF (TA328560) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mVEGF. Rabbit IgG Purified E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

VEGF-A antibody

  Biotinylated Anti-Rat VEGF Rabbit Polyclonal Antibody  
To detect Rat VEGF by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat VEGF. Rabbit IgG Purified Rt E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

VEGF-A antibody

  Anti-Rat VEGF Antibody  
To detect Rat VEGF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Rat VEGF (TA328598) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat VEGF. Rabbit Purified Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

VEGF-A antibody

  Rabbit anti-VEGFA Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using VEGFA antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

VEGF-A antibody

  Mouse Monoclonal VEGF Antibody (VG76e)  
Western Blot: VEGF Antibody (VG76e) [TA336482] - Analysis of VEGF in human kidney protein (30ug). Mouse IgG1 VG76e Purified Bov, Hu, Por, Sh C, E, ICC/IF, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

VEGF-A antibody

  Mouse Monoclonal VEGF Antibody (VG1)  
Western Blot: VEGF Antibody (VG1) [TA336487] - HUVEC and 293T Cells tested at 1:1000. Image provided by verified customer review. Mouse IgG1 VG1 Purified Can, Hu, Ms, Rt C, E, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

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