VEGF-C / Flt4-L antibody

Principal name

VEGF-C / Flt4-L antibody

Alternative names for VEGF-C / Flt4-L antibody

VEGFC, VRP, Vascular endothelial growth factor C, Vascular endothelial growth factor-related protein, Flt4 ligand

SwissProt ID

O35757 (Rat), P49767 (Human), P97953 (Mouse)

Gene ID

114111 (Vegfc), 7424 (VEGFC), 22341

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit, Mouse, Goat

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunoprecipitation (IP), Paraffin Sections (P), Frozen Sections (C), Flow Cytometry (F), ELISA (detection) (E(detection)), Neutralisation (NEUT)

Background of VEGF-C / Flt4-L antibody

Vascular endothelial growth factor C (VEGF-C), also known as Flt4 ligand (Flt4-L), vascular endothelial growth factor-related protein (VRP) and VEGF-2, is a member of the cysteine-knot growth factor superfamily and exhibits homology with VEGF-121 (32%) and PDGF (27%). However VEGF-C also possesses a 180 amino acid C-terminal cysteine-rich domain absent in other VEGFs. The major secreted form of VEGF-C is a disulfide linked antiparallel homodimeric protein containing 5 potential N-linked glycosylation sites and has a theoretical molecular weight of 44 kDa. Synthesis and secretion of VEGF-C from the cell involves a series of cleavage steps to produce the mature protein. The first step is antiparallel precursor homodimerization followed by proteolytic cleavage between Arg-227 and Ser-228 dividing the VEGF-C precursor into 2 nearly equal parts. This is followed by slow proteolytic cleavage to remove the N-terminal propeptide, with most of the VEGF-C secreted by different cell types being found as the disulfide linked antiparallel homodimeric precusor, rather than as the mature protein. The actions of VEGF-C are exerted through VEGF Receptor-2 (VEGFR-2) and VEGF Receptor-3 (VEGFR-3) however only the fully cleaved VEGF-C can activate VEGF2 receptor. Thus it is hypothesized that the complex proteolytic processing pathway of VEGF-C is used to prevent unnecessary angiogenic effects, elicited via VEGFR-2 which is present in many types of endothelia, and allows VEGF-C to signal preferentially via VEGFR-3, which has restricted expression. Please see Joukov et al., (1997) EMBO J 16:3898-3911 for further information. VEGF-C is predominantly involved in angiogenesis, vasculogenesis, lymphangiogenesis as well as an inhibitor of dendritic cell maturation. VEGF-C also possesses both mitogenic and chemotactic activity w.r.t. endothelial cells and monocytes. VEGF-C is over-expressed in a number of carcinomas such as breast and colorectal carcinoma and has been shown to enhance metastasis to regional lymph nodes and to the lungs. This is a HCX protein. HCX Expression System Details HCX proteins mimic the proteins in the human body because they are expressed from human, rather than animal, insect or bacterial cells. This process gives them human post-translational modifications. Recombinant DNA techniques allow a human protein with the correct amino acid sequence to be expressed in a non-human cell line. However, non-human cells lack the appropriate cellular machinery, such as specific glycosyltransferases, necessary to produce the correct human post-translational modifications of a protein. An extreme example is seen in E. coli cells, which produce recombinant proteins with no glycosylation, as the above figure illustrates. Rodent and yeast cells are able to glycosylate proteins, but they are still different from glycosylation in human cells. Expression System Resultant Proteins Human (e.g. K562, HEK293) Correct amino acid sequence Human post-translational modifications Rodent (e.g. CHO, NSO) Correct amino acid sequence Some natural glycosylation - not human-like Yeast (e.g. Pichia) Correct amino acid sequence Some natural glycosylation - not human-like E.Coli Correct amino acid sequence No PTMs Although there have been significant attempts to make non-human cell derived cytokines more human-like, there is a growing awareness that in many instances, particularly in therapeutics, cytokines should mimic those found in the body as closely as possible.

General readings

Hatano Y., et al. (2014) Biochem. Biophys. Res. Commun. 446:149-154.Ciobanu M., et al. (2013) Rom J Morphol. Embryol. 54:925-934.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

VEGF-C / Flt4-L antibody

Western analysis of recombinant human VEGF-C (Cat.-No AR01001PU derived from insect cells using a monoclonal antibody directed against recombinant human/rat VEGF-C. The antibody does not recognize the unreduced form. The bands correspond to different glycosylated forms. Mouse IgG2a 9/G10 Purified Hu E, WB
0.1 mg / €330.00
  Acris Antibodies GmbH

VEGF-C / Flt4-L antibody

Western blot analysis using recombinant murine VEGF-C. Lane 1: 50 ng, Lane 2: 100 ng, Lane 3: 200 ng. Rabbit Purified Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

VEGF-C / Flt4-L antibody

Predicted band size: 46 kDa . Positive control: 231 cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/200 dilution Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

VEGF-C / Flt4-L antibody

Host: Rabbit; Target Name: VEGFC; Sample Tissue: NCI-H226 Whole cell lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

VEGF-C / Flt4-L antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VEGFC (RC209451, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VEGFC. Mouse IgG1 OTI4A1 Purified Hu WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

VEGF-C / Flt4-L antibody

Figure 1. Western analysis of extracts from HDMEC cells using VEGF3 antibody. Mouse IgG1 30AT1174.77 Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

VEGF-C / Flt4-L antibody

VEGF-C Sandwich-ELISA using recombinant Human VEGF-C as standard (Cat.-No AR01001PU-N). Mouse antihuman VEGF-C Antibody Cat.-No AM33409PU-N (Clone 9/G10) was used as capture antibody, Biotinylated Mouse anti-Human VEGF-C Antibody Cat.-No AM33410BT-N (Clone 107/A11) was used for detection. Mouse IgG2a 107/A11 Biotin Hu E
50 µg / €330.00
  Acris Antibodies GmbH

VEGF-C / Flt4-L antibody

VEGF-C Sandwich-ELISA using recombinant human VEGF-C as standard (Cat.-No AR01001PU-N). Mouse anti-human VEGF-C Antibody Cat.-No AM33409PU-N (Clone 9/G10) was used as capture antibody, Biotinylated Mouse anti-Human VEGF-C Antibody Cat.-No AM33410BT-N (Clone 107/A11) was used for detection. Mouse IgG2a 107/A11 Purified Hu E, WB
0.1 mg / €330.00
  Acris Antibodies GmbH

VEGF-C / Flt4-L (Center) antibody

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with VEGF3 Antibody (Center) Cat.-No AP11908PU-N, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

VEGF-C / Flt4-L (N-term) antibody

VEGF3 Antibody (N-term) (AP54506PU-N)immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of VEGF3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu F, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

VEGF-C / Flt4-L antibody

Immunohistochemistry with paraffin-embedded section of human colon carcinoma tissue using DM3527 (Clone 9E7). Wroclaw Medical University Departmetnt of Histology and Embriology Mouse IgG2a 9E7 Purified Hu, Rt C, P, WB
0.1 mg / €330.00
  Acris Antibodies GmbH

VEGF-C / Flt4-L antibody

Western analysis of recombinant human VEGF-C derived from insect cells using Cat.-No. DM3528, a monoclonal antibody directed against recombinant human/rat VEGF-C. Mouse IgG2a 107F10 Purified Hu, Rt E, WB
0.1 mg / €330.00
  Acris Antibodies GmbH

VEGF-C / Flt4-L antibody

VEGF-C Sandwich-ELISA using the polyclonal anti-rat VEGF-C antibody (Cat.-No DP3507) as capture antibody and recombinant Rat VEGF-C (Cat.-No DA3519X) as standard. The Biotinylated rabbit anti-rat VEGF-C (Cat.No DP3507B) was used as detection antibody. Rabbit Azide Free Hu, Ms, Rt E, IP, WB
0.2 mg / €300.00
  Acris Antibodies GmbH

VEGF-C / Flt4-L antibody

VEGF-C Sandwich-ELISA using the Biotinylated polyclonal anti-Rt VEGF-C Antibody (Cat.-No DP3507B) as detection antibody and recombinant Rat VEGF-C  (Cat.-No DA3519X) as standard. The monoclonal mouse anti-Human VEGF-C Antibody was used as capture antibody. Rabbit IgG Biotin Hu, Ms, Rt E
50 µg / €300.00
  Acris Antibodies GmbH

VEGF-C / Flt4-L antibody

VEGF-C Sandwich-ELISA using the polyclonal anti-rat VEGF-C antibody (Cat.-No DP3507) as capture antibody and recombinant Rat VEGF-C (Cat.-No DA3519X) as standard. The Biotinylated rabbit anti-rat VEGF-C (Cat.No DP3507B) was used as detection antibody. Rabbit Azide Free Hu, Ms, Rt E, IP, WB
0.1 mg / €250.00
  Acris Antibodies GmbH

VEGF-C / Flt4-L antibody

VEGF-C / Flt4-L Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

VEGF-C / Flt4-L antibody

VEGF-C / Flt4-L Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

VEGF-C / Flt4-L antibody

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with VEGFC polyclonal antibody ( Cat # PAB3867 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Hu E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

VEGF-C / Flt4-L antibody

VEGF-C / Flt4-L Goat Hu C, P, WB
0.1 mg / €470.00
  Abnova Taiwan Corp.

VEGF-C / Flt4-L antibody

VEGF-C / Flt4-L Rabbit Hu, Ms, Rt E, IP
0.1 mg / €470.00
  Abnova Taiwan Corp.

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