VEGFR-3 / Flt-4 antibody

Principal name

VEGFR-3 / Flt-4 antibody

Alternative names for VEGFR-3 / Flt-4 antibody

VEGFR3, FLT4, VEGF Receptor 3, Vascular endothelial growth factor receptor 3, Tyrosine-protein kinase receptor FLT4

SwissProt ID

P35916 (Human), P35917 (Mouse), Q5MD89 (Danre), Q91ZT1 (Rat)

Gene ID

2324 (FLT4), 14257 (Flt4), 114110

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Macaque

Available hosts

Rabbit, Mouse, Rat, Chicken

Available applications

Frozen Sections (C), Paraffin Sections (P), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)

Background of VEGFR-3 / Flt-4 antibody

Recombinant human soluble Vascular Endothelial Growth Factor Receptor-3 (sVEGFR-3/FLT-4) was fused with a carboxy-terminal 6X histidine-tag. All three VEGF receptors belong to the class III subfamily of receptor tyrosine kinases (RTKs) characterised by the seven immunoglobulin-like loops in the extracellular domain. The expression of VEGFR-1 to -3 is almost exclusively restricted to hematopoietic precursor cells, vascular and lymphatic endothelial cells and to the monocyte/macrophage lineage. They play key roles in vasculogenesis, hematopoiesis, angiogenesis and lymphangiogenesis. The FLT-4 cDNA encodes a 1298 amino acid (aa) residue precursor protein with a 23 aa residue signal peptide. Mature VEGFR-3/FLT-4 is composed of a 751 aa residue extracellular domain, a 22 aa transmembrane domain and a 482 aa residue cytoplasmic domain. Both VEGF family members VEGF-C and VEGF-D have been shown to bind and activate VEGFR-3/FLT-4. The Flt-4 gene is widely expressed in the early embryo but becomes restricted to the lymphatic endothelial a latter stages of development. It is important for lymphangiogenesis.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

VEGFR-3 / Flt-4 antibody

VEGFR-3 / Flt-4 Mouse IgG1 3C3 Biotin Hu E, WB
50 µg / €350.00
  Acris Antibodies GmbH

VEGFR-3 / Flt-4 antibody

Immunofluorescence staining of human VEGFR-3/FLT-4 (red) in hFlt4-transfected MG63 cells. Monoclonal mouse anti-human FLT-4  clone 9D9 (Cat.-No DM3512P) and polyclonal rabbit anti-human FLT-4 (D) Cat.-No DP3511: A and C are negative control with secondary antibody only; B (mAb) and D (pAb) with specific antibodies against human FLT-4.The experiment was performed by the research group of Dr. Wolfgang Holnthoner, Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, Austrian Cluster for Tissue Regeneration, Donaueschingenstrasse 13, A-1200 Vienna, Austria. Mouse IgG1 (3C3) 9D9 Aff - Purified Hu C, E, ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

VEGFR-3 / Flt-4 antibody

VEGFR-3 / Flt-4 Rabbit Purified Hu C, P
1 ml / €650.00
  Acris Antibodies GmbH

VEGFR-3 / Flt-4 antibody

VEGFR-3 / Flt-4 Rabbit Purified Hu P
0.5 ml / €420.00
  Acris Antibodies GmbH

VEGFR-3 / Flt-4 antibody

  Anti-FLT4 Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm. Positive control: Hela and HepG2 cell. Recommended dilution: 1/100-500 The image is immunocytochemistry of methanol-fixed Hela cell  using FLT4 antibody at dilution 1/100, the secondary antibody is FITC conjugated Goat Anti-Rabbit IgG at dilution 1/200, blue is nucleus staining by Dapi. (Original magnification:×200) Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF
0.1 ml / €325.00
  OriGene Technologies, Inc.

VEGFR-3 / Flt-4 antibody

  Rabbit anti-FLT4 Polyclonal Antibody  
Western blot analysis of extracts of HUVEC cell line, using FLT4 antibody. Rabbit IgG Purified Hu
0.1 mg / €325.00
  OriGene Technologies, Inc.

VEGFR-3 / Flt-4 antibody

  Rabbit Polyclonal Anti-VEGFR3 Antibody  
Western blot analysis of extracts from HUVEC cells using VEGFR-3 Antibody (right) and the same antibody preincubated with blocking peptide Rabbit IgG Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

VEGFR-3 / Flt-4 antibody

  Rabbit Polyclonal VEGF Receptor 3 Antibody  
ELISA: VEGF Receptor 3 Antibody Rabbit IgG Purified Hu E
0.1 mg / €325.00
  OriGene Technologies, Inc.

VEGFR-3 / Flt-4 antibody

  FLT4 mouse monoclonal antibody, clone OTI1G2 (formerly 1G2)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FLT4 (RC214285, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FLT4. Mouse IgG1 OTI1G2 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

VEGFR-3 / Flt-4 antibody

  FLT4 mouse monoclonal antibody, clone OTI1G7 (formerly 1G7)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FLT4 (RC214285, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FLT4. Mouse IgG1 OTI1G7 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

VEGFR-3 / Flt-4 antibody

  FLT4 mouse monoclonal antibody, clone OTI8C2 (formerly 8C2)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FLT4 (RC214285, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FLT4. Mouse IgG1 OTI8C2 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

VEGFR-3 / Flt-4 antibody

Western blot analysis using FLT4 antibody Cat.-No AM06464SU-N against FLT4(AA: 25-330)-hIgGFc transfected HEK293 cell lysate. Mouse IgG1 4H4 Ascites Hu E, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

VEGFR-3 / Flt-4 antibody

Figure 2. Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with VGFR3 Monoclonal Antibody (Cat.#AM11070PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Mouse IgG1 44CT92.4.4 Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

VEGFR-3 / Flt-4 antibody

Immunofluorescence staining of VEGFR-3/FLT4 in Mouse primary endothelial cells (SnoMec) with monoclonal Rat anti-Mouse VEGFR-3/FLT4 #7A3 Cat.-No AM20378PU-N (upper panel). Control with goat anti-rat ALEXA Flour 488 (lower panel). Rat IgG1 7A3 Purified Ms ICC/IF, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

VEGFR-3 / Flt-4 antibody

Western analysis of recombinant human soluble VEGFR3/FLT-4 using a Mouse anti-Human FLT-4 antibody (Cat.-No AM20379PU-N). Mouse IgG1 2E11 Aff - Purified Hu C, E, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

VEGFR-3 / Flt-4 antibody

VEGFR-3 / Flt-4 Rat IgG2 5F63 Purified Ms P, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

VEGFR-3 / Flt-4 (1279-1283) antibody

Western blot analysis of extracts from HUVEC cells using VEGFR-3 Antibody AP26050PU-N and the same antibody preincubated with blocking peptide. Rabbit Aff - Purified Hu WB
0.1 ml / €320.00
  Acris Antibodies GmbH

VEGFR-3 / Flt-4 (1279-1283) antibody

Western blot analysis of extracts from HUVEC cells using VEGFR-3 Antibody AP26050PU-N and the same antibody preincubated with blocking peptide. Rabbit Aff - Purified Hu WB
50 µl / €240.00
  Acris Antibodies GmbH

VEGFR-3 / Flt-4 antibody

VEGFR-3 / Flt-4 Rabbit Azide Free Hu E, IP, WB
0.2 mg / €290.00
  Acris Antibodies GmbH

VEGFR-3 / Flt-4 antibody

VEGFR-3 / Flt-4 Rabbit Aff - Purified Hu E, IP, WB
50 µg / €270.00
  Acris Antibodies GmbH

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