VGAT / SLC32A1 antibody

Principal name

VGAT / SLC32A1 antibody

Alternative names for VGAT / SLC32A1 antibody

Vesicular GABA transporter, VIAAT, Vesicular inhibitory amino acid transporter, GABA and glycine transporter, Solute carrier family 32 member 1

SwissProt ID

O35458 (Rat), O35633 (Mouse), Q6PF45 (Xenla), Q9H598 (Human)

Gene ID

140679 (SLC32A1), 100364703 (LOC100364703), 83612 (Slc32a1)

Available reactivities

Hu (Human), Rt (Rat), Ms (Mouse), Bov (Bovine), Can (Canine), Ce (C. elegans)

Available hosts

Rabbit

Available applications

Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E)

Background of VGAT / SLC32A1 antibody

Synaptic transmission involves the regulated exocytosis of vesicles containing neurotransmitters. The inhibitory neurotransmitter g-aminobutyric acid (GABA) is synthesized in the cytoplasm from L-glutamate by the enzyme glutamic acid decarboxylase (GAD) and loaded in synaptic vesicles for exocytosis. The vesicular GABA transporter (VGAT, also termed vesicular inhibitory amino acid transporter or VIAAT, 52-57 kDa) is responsible for the uptake of GABA into synaptic vesicles, following synthesis by GAD, or reuptake by plasma membrane GABA transporters (GATs). VGAT protein (55 kDa) contains ten transmembrane domains and belongs to a new family of vesicular neurotransmitter transporters. VGAT is highly conserved in mouse, rat and human (95-98% identity). VGAT uses both chemical and electrical components of the luminal protein gradient to mediate the accumulation of GABA into synaptic vesicles. VGAT is also responsible for glycine uptake in glycinergic neurons. VGAT is a homolog of the c. elegans unc -47 protein involved in GABA transport. Unc-47 is expressed in GABA neurons, localizes to synaptic vesicles and confers vesicular GABA transport in transfected cells. Mutants of c. elegans in which the unc-47 gene encoding VGAT is non-functional or absent, exhibit a complete loss of GABAergic function and elevated levels of GABA in the cytoplasm. VGAT is present in nerve endings of inhibitory neurons containing GABA, but also in glycinergic neurons in the brain and retina.1,4,5 At subcellular levels, VGAT specifically associates with synaptic vesicles. VGAT/VIAAT is also expressed in rat pancreatic islet cells.

General readings

Chaudhry FA, Reimer RJ, Bellocchio EE, Danbolt NC, Osen KK, Edwards RH, Storm-Mathisen J (1998) The vesicular GABA transporter, VGAT, localizes to synaptic vesicles in sets of glycinergic as well as GABAergic neurons. J Neurosci 18:9733-9750.De GS, Schafer MK, Defamie N, Chen C, Ricci A, Weihe E, Varoqui H, Erickson JD (2005) Homeostatic scaling of vesicular glutamate and GABA transporter expression in rat neocortical circuits. J Neurosci 25:7121-7133.McIntire SL, Reimer RJ, Schuske K, Edwards RH, Jorgensen EM (1997) Identification and characterization of the vesicular GABA transporter. Nature (Lond ) 389:870-876.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

VGAT / SLC32A1 (511-525) antibody

Staining with Vesicular Inhibitory Amino Acid Transporter (VIAAT) (20092) Rabbit Serum Hu, Ms, Rt C, WB
0.1 ml / €470.00
  ImmunoStar Inc.

VGAT / SLC32A1 (N-term) antibody

Western blot of rat hippocampal lysate showing specific immunolabeling of the ~53k VGAT protein. Rabbit IgG Aff - Purified Bov, Can, non-Human Primates, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

VGAT / SLC32A1 antibody

Predicted band size: 57 kDa . Positive control: Mouse brain tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/950 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 20 seconds) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

VGAT / SLC32A1 antibody

Predicted band size: 57 kDa . Positive control: Mouse eye tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 20 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

VGAT / SLC32A1 antibody

Western blot analysis of extracts of mouse eye tissue lysate, using SLC32A1antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

VGAT / SLC32A1 antibody

Western blot analysis of rat brain membranes (lanes 1 and 4), mouse brain membranes (lanes 2 and 5) and human U-87 MG glyoblastoma lysates (lanes 3 and 6): 1-3. Anti-Vesicular GABA Transporter antibody, (1:200). 4-6. Anti-Vesicular GABA Transporter antibody, preincubated with the control peptide antigen. Rabbit Purified Hu, Ms, Rt WB
0.2 ml / €435.00
  OriGene Technologies, Inc.

VGAT / SLC32A1 antibody

WB Suggested Anti-SLC32A1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: PANC1 cell lysate Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

VGAT / SLC32A1 antibody

VGAT / SLC32A1 Rabbit Serum Hu, Rt C, ICC/IF
50 µl / €540.00
  Acris Antibodies GmbH

VGAT / SLC32A1 antibody

Western blot of rat hippocampal lysate showing specific immunolabeling of the ~53k Slc32a1 protein. Using Slc32a1 polyclonal antibody ( Cat # PAB9637 ). Rabbit Bov, Rt WB
0.1 ml / €470.00
  Abnova Taiwan Corp.

VGAT / SLC32A1 antibody

Human PANC1; WB Suggested Anti-SLC32A1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: PANC1 cell lysate; SLC32A1 antibody - N-terminal region (ARP44086_P050) in Human PANC1 cells using Western Blot Rabbit Aff - Purified Can, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

VGAT / SLC32A1 antibody

VGAT / SLC32A1 Rabbit Aff - Purified Ms, Rt WB
0.1 ml / €400.00
  Novus Biologicals Inc.

VGAT / SLC32A1 antibody

Western Blot: VGAT Antibody [NB300-740] - Rat hippocampal lysate showing specific immunolabeling of the ~53k VGAT protein. Rabbit Aff - Purified Rt WB
0.1 ml / €380.00
  Novus Biologicals Inc.

Lysates

Catalog No.    

SLC32A1 overexpression lysate

SLC32A1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

13 Item(s)

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