VGF antibody

Principal name

VGF antibody

Alternative names for VGF antibody

Neurosecretory protein VGF

SwissProt ID

O15240 (Human), P20156 (Rat)

Gene ID

7425 (VGF), 29461 (Vgf)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Dros (Drosophila), Ze (Zebrafish), Eq (Equine), Por (Porcine), Ye (Yeast), GP (Guinea Pig)

Available hosts

Rabbit

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of VGF antibody

VGF (VGF, Nerve Growth Factor-Inducible), is a protein and neuropeptide that may play a role in regulating energy homeostasis, metabolism. Canu et al. (1997) noted that rat Vgf encodes a predicted 70-kD polypeptide that shares similarities with the secretogranin/chromogranin family and is found in the secretory granules of subsets of neurons and endocrine cells. By fluorescence in situ hybridization, Canu et al. (1997) assigned the VGF gene to 7q22. Canu et al. (1997) demonstrated that the single-copy human VGF gene spans 6 kb of genomic DNA and contains 2 exons. The entire VGF protein is encoded by exon 2, while exon 1 contains only 5-prime untranslated sequence. The structural organization of the human gene is similar to that described for the rat VGF gene (Salton et al., 1991), and both the translated and the untranslated regions show a high degree of sequence homology to the rat gene.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

VGF antibody

Western blot analysis of VGF in human brain tissue lysate with VGF antibody at (A) 0.5 and (B) 1 ug/ml. Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

VGF antibody

Western blot analysis of extracts from RAW264.7 cells, using VGF antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

VGF antibody

WB Suggested Anti-VGF Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Transfected 293T Rabbit IgG Purified Bov, Can, GP, Hu, Ms, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

VGF antibody

WB Suggested Anti-VGF Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: THP-1 cell lysate Rabbit IgG Purified Can, Eq, Hu, Ms, Por, Rt, Ye, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

VGF antibody

Gel: 8%SDS-PAGE,Lysate: 40 ug,,Primary antibody: (VGF Antibody) at dilution 1/500 dilution,Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,Exposure time: 1 minute Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

VGF antibody

Immunohistochemistry (IHC) analyzes of VGF antibody (Cat.-No.: AP01489PU-N) in paraffin-embedded human lung carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

VGF (C-term) antibody

Human Brain, Cortex (formalin-fixed, paraffin-embedded) stained with VGF antibody AP22596PU-N at 5 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit Aff - Purified Hu, Ms, Rt P, WB
50 µg / €470.00
  Acris Antibodies GmbH

VGF Control Peptide

VGF
50 µg / €160.00
  Acris Antibodies GmbH

VGF (C-term) antibody

Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

VGF antibody

Immunohistochemistry of VGF in human brain with VGF polyclonal antibody ( Cat # PAB13036 ) at 5 µg/ml. Rabbit Hu, Ms, Rt P, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

VGF antibody

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using VGF Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €205.00
  Assay Biotech

VGF antibody

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using VGF Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €310.00
  Assay Biotech

VGF antibody

Transfected 293T; WB Suggested Anti-VGF Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Transfected 293T; VGF antibody - middle region (ARP42127_P050) in Transfected 293T cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

VGF antibody

Human THP-1; WB Suggested Anti-VGF Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: THP-1 cell lysate; VGF antibody - middle region (ARP42128_P050) in Human THP-1 cells using Western Blot Rabbit Aff - Purified Can, Dros, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

VGF antibody

VGF Rabbit IgG Aff - Purified Hu, Ms, Rt WB
0.1 mg / €290.00
  Boster Immunoleader

VGF antibody

Immunohistochemical analysis of VGF staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Hu, Ms, Rt ICC/IF, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

VGF antibody

Immunohistochemical analysis of VGF staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

VGF antibody

Immunohistochemical analysis of VGF staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Hu, Ms, Rt ICC/IF, P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

VGF

VGF Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

VGF overexpression lysate

VGF overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

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