VHL antibody

Principal name

VHL antibody

Alternative names for VHL antibody

Von Hippel-Lindau disease tumor suppressor, Protein G7

SwissProt ID

P40337 (Human), P40337-2, P40338 (Mouse), Q5Q9Z2 (Canfa), Q64259 (Rat), Q9V3C1 (Drome)

Gene ID

7428 (VHL), 22346 (Vhl), 24874

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine)

Available hosts

Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Frozen Sections (C), Immunoprecipitation (IP)

Background of VHL antibody

The Von Hippel-Lindau (VHL) protein is a substrate recognition component of an E3 ubiquitin ligase complex containing elongin BC (TCEB1 and TCEB2), cullin 1 (CUL1), and RING-box protein 1 (RBX1). VHL protein has been shown to exist as three distinct isoforms resulting from altertively spliced transcript variants. Loss of VHL protein function results in a domintly inherited familial cancer syndrome that manifests as angiomas of the reti, hemangioblastomas of the central nervous system, rel clear-cell carcinomas and pheochromocytomas. Under normoxic conditions, VHL directs the ubiquitylation and subsequent proteosomal degradation of the hypoxia inducible factor HIF alpha, maintaining very low levels of HIF alpha in the cell. Cellular exposure to hypoxic conditions, or loss of VHL protein function, results in increased HIF alpha protein levels and increased expression of HIF-induced gene products, many of which are angiogenesis factors such as vascular endothelial growth factor (VEGF). Thus, loss of VHL protein function is believed to contribute to the formation of highly vascular neoplasias. In addition to HIF alpha, VHL is known to regulate the ubiquitylation of several other proteins, including tat-binding protein 1 (TBP-1), the atypical protein kise C lambda (aPKC), and two subunits of the multiprotein R Polymerase II complex (RPB1 and RPB7). Interactions with elongin BC, RPB1, RPB7 and the pVHL-associated KRAB-A domain containing protein (VHLaK) suggest that VHL may also play a more direct role in transcriptiol repression.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

VHL antibody

  VHL (Von Hippel Lindau) mouse monoclonal antibody, clone OTI1E1 (formerly 1E1)  
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-VHL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506222) Mouse IgG2b OTI1E1 Purified Hu P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

VHL antibody

  Goat Polyclonal Antibody against VHL  
TA303127 (0.01µg/ml) staining of Human Ovary lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt E, WB
0.1 mg / €360.00
  OriGene Technologies, Inc.

VHL antibody

  Anti-VHL Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm. Positive control: Human cervical and thyroid cancer tissue. Recommended dilution: 1/50-200  The image on the left is immunohistochemistry of paraffin-embedded human cervical cancer tissue using VHL antibody at dilution 1/40, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €335.00
  OriGene Technologies, Inc.

VHL antibody

  Anti-VHL Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm. Positive control: Human colon and thyroid cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using VHL antibody at dilution 1/40, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €335.00
  OriGene Technologies, Inc.

VHL (N-term) antibody

  Rabbit polyclonal VHL Antibody (N-term)  
Western blot analysis of VHL antibody (N-term) (Cat. #TA324848) in HepG2 cell line lysates (35ug/lane). VHL (arrow) was detected using the purified Pab. Rabbit Hu WB
0.4 ml / €410.00
  OriGene Technologies, Inc.

VHL antibody

  Rabbit anti-VHL Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using VHL antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €335.00
  OriGene Technologies, Inc.

VHL antibody

  Rabbit Polyclonal VHL Antibody  
VHL Rabbit IgG Purified Hu, Ms, Rt P
0.1 ml / €335.00
  OriGene Technologies, Inc.

VHL (C-term) antibody

Western blot analysis of VHL antibody (C-term) (AP17826PU-N) in HepG2 cell line lysates (35ug/lane). VHL (arrow) was detected using the purified Pab. Rabbit Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

VHL antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using VHL (Ab-68) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P
50 µg / €230.00
  Assay Biotech

VHL antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using VHL (Ab-68) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P
0.1 mg / €330.00
  Assay Biotech

CRISPR

Catalog No. Species Donor Vector  

VHL - human gene knockout kit via CRISPR

VHL - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

VHL - human gene knockout kit via CRISPR

VHL - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

VHL - human gene knockout kit via CRISPR

VHL - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

VHL - human gene knockout kit via CRISPR

VHL - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Vhl - mouse gene knockout kit via CRISPR

Vhl - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Vhl - mouse gene knockout kit via CRISPR

Vhl - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Vhl - mouse gene knockout kit via CRISPR

Vhl - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Vhl - mouse gene knockout kit via CRISPR

Vhl - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

VHL (transcript variant 1)

  Recombinant protein of human von Hippel-Lindau tumor suppressor (VHL), transcript variant 1  
VHL Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €438.00
  OriGene Technologies, Inc.

VHL (full length, N-term HIS tag, transcript variant 1)

  Purified recombinant protein of Human von Hippel-Lindau tumor suppressor (VHL), transcript variant 1, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug  
VHL Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

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