VHL antibody

Principal name

VHL antibody

Alternative names for VHL antibody

Von Hippel-Lindau disease tumor suppressor, Protein G7

SwissProt ID

P40337 (Human), P40337-2, P40338 (Mouse), Q5Q9Z2 (Canfa), Q64259 (Rat), Q9V3C1 (Drome)

Gene ID

7428 (VHL)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP), ELISA (detection) (E(detection)), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of VHL antibody

The Von Hippel-Lindau (VHL) protein is a substrate recognition component of an E3 ubiquitin ligase complex containing elongin BC (TCEB1 and TCEB2), cullin 1 (CUL1), and RING-box protein 1 (RBX1). VHL protein has been shown to exist as three distinct isoforms resulting from altertively spliced transcript variants. Loss of VHL protein function results in a domintly inherited familial cancer syndrome that manifests as angiomas of the reti, hemangioblastomas of the central nervous system, rel clear-cell carcinomas and pheochromocytomas. Under normoxic conditions, VHL directs the ubiquitylation and subsequent proteosomal degradation of the hypoxia inducible factor HIF alpha, maintaining very low levels of HIF alpha in the cell. Cellular exposure to hypoxic conditions, or loss of VHL protein function, results in increased HIF alpha protein levels and increased expression of HIF-induced gene products, many of which are angiogenesis factors such as vascular endothelial growth factor (VEGF). Thus, loss of VHL protein function is believed to contribute to the formation of highly vascular neoplasias. In addition to HIF alpha, VHL is known to regulate the ubiquitylation of several other proteins, including tat-binding protein 1 (TBP-1), the atypical protein kise C lambda (aPKC), and two subunits of the multiprotein R Polymerase II complex (RPB1 and RPB7). Interactions with elongin BC, RPB1, RPB7 and the pVHL-associated KRAB-A domain containing protein (VHLaK) suggest that VHL may also play a more direct role in transcriptiol repression.

General readings

Latif, F., et al (1993) Science. 260(5112),1317-1320
Duan,D.R., et al (1995) Proc.Natl.Acad.Sci. U.S.A. 92(14),6459-6463
Maxwell,P.H., et al (1999) Nature 399(6733), 271-275
Chales E. Stebbins, et al (1999) Science 284, 455-461
Staller, P. et al (2003) Nature 425, 307-311

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

VHL antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-VHL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506222) Mouse IgG2b OTI1E1 Purified Hu P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

VHL antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-VHL Rabbit Polyclonal antibody. (TA590023) Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

VHL antibody

TA303127 (0.01µg/ml) staining of Human Ovary lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt WB
20 µg / €200.00
  Acris Antibodies GmbH

VHL antibody

TA303127 (0.01µg/ml) staining of Human Ovary lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

VHL antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue using VHL antibody.The picture on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

VHL antibody

Predicted cell location: Cytoplasm. Positive control: Human cervical and thyroid cancer tissue. Recommended dilution: 1/50-200  The image on the left is immunohistochemistry of paraffin-embedded human cervical cancer tissue using VHL antibody at dilution 1/40, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

VHL antibody

Predicted cell location: Cytoplasm. Positive control: Human colon and thyroid cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using VHL antibody at dilution 1/40, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

VHL (N-term) antibody

Western blot analysis of VHL antibody (N-term) (Cat. #TA324848) in HepG2 cell line lysates (35ug/lane). VHL (arrow) was detected using the purified Pab. Rabbit Hu WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

VHL antibody

Western blot analysis of extracts of various cell lines, using VHL antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

VHL antibody

VHL Rabbit IgG Purified Hu, Ms, Rt P
0.1 ml / €325.00
  OriGene Technologies, Inc.

VHL antibody

Figure 1. Immunohistochemical staining using VHL antibody (AM05321PU-N) on formalin fixed, paraffin embedded human kidney tissue. Mouse IgG1 Ig33 Purified Hu C, IP, P, WB
0.1 mg / €570.00
  Acris Antibodies GmbH

VHL (C-term) antibody

Western blot analysis of VHL antibody (C-term) (AP17826PU-N) in HepG2 cell line lysates (35ug/lane). VHL (arrow) was detected using the purified Pab. Rabbit Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

VHL antibody

Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Purified Hu, Ms, Rt E, P
50 µg / €470.00
  Acris Antibodies GmbH

VHL antibody

Immunohistochemistry: SM6010 VHL antibody staining of Formalin-Fixed, Paraffin-Embedded Human Pancreas at 15 µg/ml followed by biotinylated anti-Mouse IgG secondary antibody, Alkaline Phosphatase-Streptavidin and chromogen. Mouse IgG2b 52A11 Purified Hu E, P, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

VHL antibody

Immunohistochemistry: SM6010 VHL antibody staining of Formalin-Fixed, Paraffin-Embedded Human Pancreas at 15 µg/ml followed by biotinylated anti-Mouse IgG secondary antibody, Alkaline Phosphatase-Streptavidin and chromogen. Mouse IgG2b 52A11 Purified Hu E, P, WB
50 µl / €230.00
  Acris Antibodies GmbH

VHL antibody

VHL Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

VHL antibody

VHL MaxPab polyclonal antibody. Western Blot analysis of VHL expression in human placenta. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

VHL antibody

VHL MaxPab polyclonal antibody. Western Blot analysis of VHL expression in human placenta. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

VHL antibody

VHL MaxPab rabbit polyclonal antibody. Western Blot analysis of VHL expression in mouse lung. Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

VHL antibody

Detection limit for recombinant GST tagged VHL is approximately 0.1ng/ml as a capture antibody. Mouse IgG2b 1G12 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

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