VHL antibody
Principal name
VHL antibody
Alternative names for VHL antibody
Von Hippel-Lindau disease tumor suppressor, Protein G7
Gene ID
7428 (VHL)
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP), ELISA (detection) (E(detection)), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of VHL antibody
The Von Hippel-Lindau (VHL) protein is a substrate recognition component of an E3 ubiquitin ligase complex containing elongin BC (TCEB1 and TCEB2), cullin 1 (CUL1), and RING-box protein 1 (RBX1). VHL protein has been shown to exist as three distinct isoforms resulting from altertively spliced transcript variants. Loss of VHL protein function results in a domintly inherited familial cancer syndrome that manifests as angiomas of the reti, hemangioblastomas of the central nervous system, rel clear-cell carcinomas and pheochromocytomas. Under normoxic conditions, VHL directs the ubiquitylation and subsequent proteosomal degradation of the hypoxia inducible factor HIF alpha, maintaining very low levels of HIF alpha in the cell. Cellular exposure to hypoxic conditions, or loss of VHL protein function, results in increased HIF alpha protein levels and increased expression of HIF-induced gene products, many of which are angiogenesis factors such as vascular endothelial growth factor (VEGF). Thus, loss of VHL protein function is believed to contribute to the formation of highly vascular neoplasias. In addition to HIF alpha, VHL is known to regulate the ubiquitylation of several other proteins, including tat-binding protein 1 (TBP-1), the atypical protein kise C lambda (aPKC), and two subunits of the multiprotein R Polymerase II complex (RPB1 and RPB7). Interactions with elongin BC, RPB1, RPB7 and the pVHL-associated KRAB-A domain containing protein (VHLaK) suggest that VHL may also play a more direct role in transcriptiol repression.
General readings
Latif, F., et al (1993) Science. 260(5112),1317-1320
Duan,D.R., et al (1995) Proc.Natl.Acad.Sci. U.S.A. 92(14),6459-6463
Maxwell,P.H., et al (1999) Nature 399(6733), 271-275
Chales E. Stebbins, et al (1999) Science 284, 455-461
Staller, P. et al (2003) Nature 425, 307-311
Detection of VHL in human kidney samples (30 ug)using NB 100-485. ECL detection 10 seconds. Lane 1: 0.5 ug/ml primary Lane 2: 2 ug/ml primary
Detection limit for recombinant GST tagged VHL is approximately 0.1ng/ml as a capture antibody.
Western Blot: VHL Antibody [NB100-41384] - NB100-41384 (0.01ug/ml) staining of Human Ovary lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Hippel Lindau antibody [SPM324], prediluted (GTX17768)
VHL MaxPab polyclonal antibody. Western Blot analysis of VHL expression in human placenta.
Western Blot analysis of VHL expression in transfected 293T cell line (H00007428-T01) by VHL MaxPab polyclonal antibody.Lane 1: VHL transfected lysate(18.92 KDa).Lane 2: Non-transfected lysate.
Figure 1. Immunohistochemical staining using VHL antibody (AM05321PU-N) on formalin fixed, paraffin embedded human kidney tissue.
VHL polyclonal antibody ( Cat # PAB7071 ) ( 0.01 µg/ml ) staining of human ovary lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
Western blot analysis of HepG2 cell lysate was resolved by SDS - PAGE , transferred to PVDF membrane and probed with VHL monoclonal antibody , clone 52A11 ( 1 : 500 ) ( Cat # MAB1074 ) . Protein was visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system.
VHL MaxPab polyclonal antibody. Western Blot analysis of VHL expression in human placenta.
Western Blot analysis of VHL expression in transfected 293T cell line (H00007428-T01) by VHL MaxPab polyclonal antibody.Lane 1: VHL transfected lysate(18.92 KDa).Lane 2: Non-transfected lysate.
AP16520PU-N (0.01µg/ml) staining of Human Ovary lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
AP16520PU-N VHL antibody (1.5 µg/ml) as the reporter with a capture Rabbit antibody (2.5 µg/ml).
Immunohistochemical staining of VHL on formalin fixed, paraffin embedded human kidney tissue with VHL monoclonal antibody, clone Ig33 ( Cat # MAB1825 ).
Western blot analysis of VHL in human kidney samples ( 30 µg ) using VHL polyclonal antibody ( Cat # PAB12546 ). Lane 1 : 0.5 µg/ml primary. Lane 2 : 2 µg/ml primary.
Western Blot: Von Hippel Lindau Lysate [NBL1-17719] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for VHL
VHL antibody detects VHL protein at on U373 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U373 xenograft. VHL antibody (GTX101087) dilution: 1:500.
VHL antibody detects VHL protein at cytosol and nucleus on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. VHL antibody (GTX101087) dilution: 1:500.
VHL antibody detects VHL protein at cytosol on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. VHL antibody (GTX101087) dilution: 1:500.
VHL antibody detects VHL protein at cytosol and nucleus on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. VHL antibody (GTX101087) dilution: 1:500.
VHL antibody detects VHL protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: VHL protein stained by VHL antibody (GTX101087) diluted at 1:500.Blue: Hoechst 33342 staining.
Sample(30 μg of whole cell lysate)A:MOLT4(GTX27912)12% SDS PAGEGTX101087 diluted at 1:2000
VHL antibody detects VHL protein by Western blot analysis.A. 30 µg Neuro2A whole cell lysate/extract B. 30 µg GL261 whole cell lysate/extract 12 % SDS-PAGEVHL antibody (GTX101087) dilution: 1:1000
VHL MaxPab rabbit polyclonal antibody. Western Blot analysis of VHL expression in mouse lung.
Western Blot analysis of VHL expression in transfected 293T cell line (H00007428-T02) by VHL MaxPab polyclonal antibody.Lane 1: VHL transfected lysate(19.70 KDa).Lane 2: Non-transfected lysate.
Western blot analysis of VHL antibody (C-term) (AP17826PU-N) in HepG2 cell line lysates (35ug/lane). VHL (arrow) was detected using the purified Pab.
Western blot analysis of VHL antibody (N-term) (AP17827PU-N) in HepG2 cell line lysates (35ug/lane). VHL (arrow) was detected using the purified Pab.
ELISA: Von Hippel Lindau Antibody [23190002]
Human HeLa; WB Suggested Anti-VHL Antibody. . Titration: 1.0 ug/ml. . Positive Control: Hela Whole Cell; VHL antibody - N-terminal region (ARP59367_P050) in Human HeLa cells using Western Blot
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Immunohistochemical of paraffin-embedded human kidney using 16538-1-AP(VHL antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemical of paraffin-embedded human kidney using 16538-1-AP(VHL antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of HepG2 cells, using VHL antibody 16538-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Immunofluorescent analysis of HepG2 cells using 24756-1-AP( VHL Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Western blot analysis of Von Hippel Lindau expression in HepG2 (A); HEK293T (B); A549 (C); rat brain (D) whole cell lysates.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-VHL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506222)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-VHL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506222)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-VHL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506222)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-VHL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506222)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-VHL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506222)
Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-VHL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506222)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-VHL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506222)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VHL (RC216151, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VHL.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-VHL Rabbit Polyclonal antibody. (TA590023)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-VHL Rabbit Polyclonal antibody. (TA590023)
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-VHL Rabbit Polyclonal antibody. (TA590023)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-VHL Rabbit Polyclonal antibody. (TA590023)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-VHL Rabbit Polyclonal antibody. (TA590023)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-VHL Rabbit Polyclonal antibody. (TA590023)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-VHL Rabbit Polyclonal antibody. (TA590023)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VHL (RC216151, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VHL.
ELISA: Von Hippel Lindau Antibody
- Quick-Filters:
- Highly Validated (2)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA506222 | VHL antibody |
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Mouse | IgG2b | OTI1E1 | Purified | Hu | P, WB |
0.1 ml /
€379.00
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| OriGene Technologies, Inc. | |||||||
 +7 additional images |
Top Selling product! Highly validated antibody!
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| TA590023 | VHL antibody |
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 |
Rabbit | IgG | Purified | Hu | E, P, WB |
0.1 mg /
€325.00
|
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| OriGene Technologies, Inc. | |||||||
| +8 additional images |
Highly validated antibody! |
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| AP16520PU-T | VHL antibody |
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Goat | Aff - Purified | Can, Hu, Ms, Rt | WB |
20 µg /
€200.00
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| Acris Antibodies GmbH | |||||||
Acris recommends this product for Western blotting. |
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| TA303127 | VHL antibody |
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Goat | Aff - Purified | Can, Hu, Ms, Rt | E, WB |
0.1 mg /
€325.00
|
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| OriGene Technologies, Inc. | |||||||
| TA311788 | VHL antibody |
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 |
Rabbit | IgG | Purified | Hu, Ms | E, P |
0.1 ml /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA321159 | VHL antibody |
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 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA321160 | VHL antibody |
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 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA324848 | VHL (N-term) antibody |
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 |
Rabbit | Hu | WB |
0.4 ml /
€325.00
|
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| OriGene Technologies, Inc. | |||||||
| +3 additional images |
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| TA326834 | VHL antibody |
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 |
Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 mg /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA327866 | VHL antibody |
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Rabbit | IgG | Purified | Hu, Ms, Rt | P |
0.1 ml /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| AM05321PU-N | VHL antibody |
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 |
Mouse | IgG1 | Ig33 | Purified | Hu | C, IP, P, WB |
0.1 mg /
€570.00
|
| Acris Antibodies GmbH | |||||||
| AP17826PU-N | VHL (C-term) antibody |
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 |
Rabbit | Hu | WB |
0.4 ml /
€370.00
|
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| Acris Antibodies GmbH | |||||||
| AP31260PU-N | VHL antibody |
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 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
50 µg /
€470.00
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|
| Acris Antibodies GmbH | |||||||
| SM6010 | VHL antibody |
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 |
Mouse | IgG2b | 52A11 | Purified | Hu | E, P, WB |
0.1 ml /
€310.00
|
| Acris Antibodies GmbH | |||||||
| +1 additional image |
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| SM6010S | VHL antibody |
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Mouse | IgG2b | 52A11 | Purified | Hu | E, P, WB |
50 µl /
€230.00
|
| Acris Antibodies GmbH | |||||||
| +1 additional image |
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| H00007428-A01 | VHL antibody |
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|
Mouse | Serum | Hu | E, WB |
50 µl /
€290.00
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| Abnova Taiwan Corp. | |||||||
| H00007428-B01 | VHL antibody |
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 |
Mouse | Serum | Hu | E(detection), WB |
50 µl /
€370.00
|
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| Abnova Taiwan Corp. | |||||||
| +1 additional image |
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| H00007428-B01P | VHL antibody |
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 |
Mouse | Purified | Hu | E(detection), WB |
50 µg /
€370.00
|
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| Abnova Taiwan Corp. | |||||||
| +1 additional image |
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| H00007428-D01P | VHL antibody |
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 |
Rabbit | Purified | Hu, Ms | E(detection), WB |
0.1 mg /
€380.00
|
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| Abnova Taiwan Corp. | |||||||
| +1 additional image |
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| H00007428-M01 | VHL antibody |
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 |
Mouse | IgG2b | 1G12 | Purified | Hu | E, WB |
0.1 mg /
€370.00
|
| Abnova Taiwan Corp. | |||||||
