Vigilin / HDLBP antibody
Principal name
Vigilin / HDLBP antibody
Alternative names for Vigilin / HDLBP antibody
HDL-binding protein, High density lipoprotein-binding protein
Gene ID
3069 (HDLBP)
Ncbi ID
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P)
Background of Vigilin / HDLBP antibody
HDL-binding protein 1 (HDLBP) is a novel protein that may be involved in the regulation of the delivery of fats to cells for energy and storage. Digested fats travel to the small intestine, where they are packaged into chylomicrons (particles filled with triglycerides). Chylomicrons then travel through the bloodstream and deliver triglycerides to tissues that are hungry for fuel or to adipose tissue for energy storage. Triglycerides are broken down or hydrolyzed by the enzyme lipoprotein lipase (LpL). The triglyceride breakdown products are then taken up and used by cells. HDLBP is the molecule in capillaries that facilitates the capture of chylomicrons and facilitates the interaction with LpL. It has been shown that fats in the bloodstream are not readily metabolized in the absence of HDLBP.
General readings
Chen, S.N., et al. BMC Med. Genet. 10, 111 (2009)
Zhou, J., et al. RNA 14(9):1773-1781(2008)
Rikova, K., et al. Cell 131(6):1190-1203(2007)
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007)
Human Jurkat; WB Suggested Anti-HDLBP Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Jurkat cell lysate; HDLBP antibody - N-terminal region (ARP40581_P050) in Human Jurkat cells using Western Blot
Western Blot: HDLBP Antibody [NB110-55293] - Detection of HDLBP in CHO transfected lysate using NB110-55293. Lane 1: empty vector Lane 2: HDLBP transfected lysate
Western blot analysis of Hdlbp in rat kidney tissue lysate. The antibody recognizes ~19-36 kDa and 80-110 kDa bands of Hdlbp in samples from human, mouse and rat, origins.
Western blot analysis of Hdlbp expression in lysatefrom Jurkat cells ( Lane 1 ) , NIH/3T3 cells ( Lane 2 ) and rat kidney ( lane 3 ) . The antibody recognizes ~68 kDa of Hdlbp in samples from human, mouse and rat origins.
Sample (30 ug of whole cell lysate) A: Hela B: Hep G2 (GTX27900) C: Raji 5% SDS PAGE GTX112369 diluted at 1:1000
Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 5% SDS PAGE GTX112369 diluted at 1:1000
Sample (30 ug of whole cell lysate) A: PC-12 5% SDS PAGE GTX112369 diluted at 1:1000
Immunofluorescent analysis of MCF-7 cells, using HDLBP antibody 15406-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15406-1-AP(HDLBP antibody) at dilution of 1:500
Jurkat cells were subjected to SDS PAGE followed by western blot with 15406-1-AP(HDLBP antibody) at dilution of 1:500
IP Result of anti-HDLBP (IP:15406-1-AP, 4ug; Detection:15406-1-AP 1:500) with Jurkat cells lysate 2400ug.
ELISA: HDLBP1/GPIHBP1 Antibody [36440002]
Human HT1080; WB Suggested Anti-HDLBP Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: HT1080 cell lysate; HDLBP antibody - middle region (ARP40582_P050) in Human HT1080 cells using Western Blot
Western Blot: HDLBP Antibody [NBP1-57304] - This Anti-HDLBP antibody was used in Western Blot of HepG2 tissue lysate at a concentration of 0.25ug/ml.
Immunohistochemistry in formalin-fixed and paraffin-embedded human lung carcinoma reacted with Vigilin / HDLBP Antibody (Center) Cat.-No AP52025PU-N, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of Vigilin / HDLBP Antibody (Center) Cat.-No AP52025PU-N in A2058, T47D cell line lysates (35ug/lane). HDLBP (arrow) was detected using the purified Pab.
Immunohistochemistry-Paraffin: HDLBP Antibody [NBP1-83287] - Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
Immunofluorescence: HDLBP Antibody [NBP1-83287] Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoplasm.
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-HDLBP rabbit polyclonal antibody. (TA590332)
Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
Immunofluorescent staining of human cell line U-2 OS shows positivity in endoplasmic reticulum.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDLBP (RC200716, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDLBP.
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11; Lane 2: RT-4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: TonsilThis validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
ELISA: HDLBP1/GPIHBP1 Antibody
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA590332 | Vigilin / HDLBP antibody |
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Rabbit | IgG | Purified | Hu | E, ICC/IF, P, WB |
50 µg /
€325.00
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| OriGene Technologies, Inc. | |||||||
 +5 additional images |
Highly validated antibody! |
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| TA345915 | Vigilin / HDLBP antibody |
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Rabbit | IgG | Purified | Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt | WB |
50 µg /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA345916 | Vigilin / HDLBP antibody |
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 |
Rabbit | IgG | Purified | Bov, Can, Eq, GP, Hu, Ms, Rb, Rt | WB |
50 µg /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| AP52025PU-N | Vigilin / HDLBP (Center) antibody |
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Rabbit | Ig | Aff - Purified | Hu | P, WB |
0.4 ml /
€370.00
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| Acris Antibodies GmbH | |||||||
| +1 additional image |
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| H00003069-B01P | Vigilin / HDLBP antibody |
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Mouse | Hu | WB |
0.5 mg /
€1,400.00
|
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| Abnova Taiwan Corp. | |||||||
| PAB8757 | Vigilin / HDLBP antibody |
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Rabbit | Chk, Hu, Ms, Rt | WB |
0.1 mg /
€400.00
|
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| Abnova Taiwan Corp. | |||||||
| PAB8762 | Vigilin / HDLBP antibody |
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Rabbit | Hu, Ms, Rt | WB |
0.2 mg /
€400.00
|
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| Abnova Taiwan Corp. | |||||||
| ARP40581_P050 | Vigilin / HDLBP antibody |
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Rabbit | Aff - Purified | African clawed frog, Bov, Can, Chk, Hu, Ms, Por, Rt | WB |
50 µg /
€350.00
|
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| AVIVA Systems Biology | |||||||
| ARP40582_P050 | Vigilin / HDLBP antibody |
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 |
Rabbit | Aff - Purified | Hu, Ms, Rt | WB |
50 µg /
€350.00
|
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| AVIVA Systems Biology | |||||||
| 36440002 | Vigilin / HDLBP antibody |
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![ELISA: HDLBP1/GPIHBP1 Antibody [36440002]](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/36440002-T01308A01.jpg) |
Rabbit | Hu | E, P, WB |
50 µg /
€400.00
|
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| Novus Biologicals Inc. | |||||||
| NB110-55293 | Vigilin / HDLBP antibody |
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![Western Blot: HDLBP Antibody [NB110-55293] - Detection of HDLBP in CHO transfected lysate using NB110-55293. Lane 1: empty vector Lane 2: HDLBP transfected lysate](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/p_55293_1.jpg) |
Rabbit | Aff - Purified | Hu | WB |
0.1 ml /
€340.00
|
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| Novus Biologicals Inc. | |||||||
| NB110-55293B | Vigilin / HDLBP antibody |
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Rabbit | Biotin | Hu | WB |
0.1 ml /
€360.00
|
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| Novus Biologicals Inc. | |||||||
| NB110-55293G | Vigilin / HDLBP antibody |
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Rabbit | DyLight488 | Hu | WB |
0.1 ml /
€390.00
|
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| Novus Biologicals Inc. | |||||||
| NB110-55293H | Vigilin / HDLBP antibody |
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Rabbit | HRP | Hu | WB |
0.1 ml /
€390.00
|
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| Novus Biologicals Inc. | |||||||
| NB110-55293PEP | Vigilin / HDLBP Control Peptide |
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Aff - Purified | Hu |
50 µg /
€140.00
|
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| Novus Biologicals Inc. | |||||||
| NB110-55293SS | Vigilin / HDLBP antibody |
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Rabbit | Aff - Purified | Hu | WB |
25 µl /
€140.00
|
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| Novus Biologicals Inc. | |||||||
| NBP1-57304 | Vigilin / HDLBP antibody |
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![Western Blot: HDLBP Antibody [NBP1-57304] - This Anti-HDLBP antibody was used in Western Blot of HepG2 tissue lysate at a concentration of 0.25ug/ml.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/HDLBP Antibody-Western Blot-NBP1-57304-img0001.jpg) |
Rabbit | Aff - Purified | Hu, Rt | WB |
50 µg /
€440.00
|
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| Novus Biologicals Inc. | |||||||
| NBP1-83287 | Vigilin / HDLBP antibody |
||||||
![Immunohistochemistry-Paraffin: HDLBP Antibody [NBP1-83287] - Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/HDLBP Antibody-Immunohistochemistry-Paraffin-NBP1-83287-0005.jpg) |
Rabbit | IgG | Aff - Purified | Hu | ICC/IF, P |
0.1 ml /
€500.00
|
|
| Novus Biologicals Inc. | |||||||
| +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP321641 | Vigilin / HDLBP (transcript variant 1) |
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€680.00
|
|
| OriGene Technologies, Inc. | |||||
