Vigilin / HDLBP antibody
Principal name
Vigilin / HDLBP antibody
Alternative names for Vigilin / HDLBP antibody
HDL-binding protein, High density lipoprotein-binding protein
Ncbi ID
NP_005327, ABM82813.1, NP_005327.1, NP_598569.1, NM_133808, NM_203346, NM_005336
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of Vigilin / HDLBP antibody
- Kit Component:
- - KN307635G1, Hdlbp gRNA vector 1 in pCas-Guide vector
- KN307635G2, Hdlbp gRNA vector 2 in pCas-Guide vector
- KN307635D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Chen, S.N., et al. BMC Med. Genet. 10, 111 (2009)
Zhou, J., et al. RNA 14(9):1773-1781(2008)
Rikova, K., et al. Cell 131(6):1190-1203(2007)
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007)
Human Jurkat; WB Suggested Anti-HDLBP Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Jurkat cell lysate; HDLBP antibody - N-terminal region (ARP40581_P050) in Human Jurkat cells using Western Blot
Western blot analysis of Hdlbp in rat kidney tissue lysate. The antibody recognizes ~19-36 kDa and 80-110 kDa bands of Hdlbp in samples from human, mouse and rat, origins.
Western blot analysis of Hdlbp expression in lysatefrom Jurkat cells ( Lane 1 ) , NIH/3T3 cells ( Lane 2 ) and rat kidney ( lane 3 ) . The antibody recognizes ~68 kDa of Hdlbp in samples from human, mouse and rat origins.
Human HT1080; WB Suggested Anti-HDLBP Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: HT1080 cell lysate; HDLBP antibody - middle region (ARP40582_P050) in Human HT1080 cells using Western Blot
Immunohistochemistry in formalin-fixed and paraffin-embedded human lung carcinoma reacted with Vigilin / HDLBP Antibody (Center) Cat.-No AP52025PU-N, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of Vigilin / HDLBP Antibody (Center) Cat.-No AP52025PU-N in A2058, T47D cell line lysates (35ug/lane). HDLBP (arrow) was detected using the purified Pab.
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-HDLBP rabbit polyclonal antibody. (TA590332)
Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
Immunofluorescent staining of human cell line U-2 OS shows positivity in endoplasmic reticulum.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDLBP (RC200716, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDLBP.
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11; Lane 2: RT-4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: TonsilThis validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
ELISA: HDLBP1/GPIHBP1 Antibody
WB Suggested Anti-HDLBP Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Jurkat cell lysate
WB Suggested Anti-HDLBP Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: HT1080 cell lysate
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA590332 | Vigilin / HDLBP antibody |
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| Rabbit Polyclonal HDLBP Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | E, ICC/IF, P, WB |
50 µg /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
 +5 additional images |
Highly validated antibody! |
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| TA345915 | Vigilin / HDLBP antibody |
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| Rabbit Polyclonal Anti-HDLBP Antibody | |||||||
 |
Rabbit | IgG | Purified | Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt | WB |
50 µg /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA345916 | Vigilin / HDLBP antibody |
||||||
| Rabbit Polyclonal Anti-HDLBP Antibody | |||||||
 |
Rabbit | IgG | Purified | Bov, Can, Eq, GP, Hu, Ms, Rb, Rt | WB |
50 µg /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA351242 | Vigilin / HDLBP antibody |
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| Rabbit Polyclonal Anti-HDLBP Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| AP52025PU-N | Vigilin / HDLBP (Center) antibody |
||||||
 |
Rabbit | Ig | Aff - Purified | Hu | P, WB |
0.4 ml /
€370.00
|
|
| Acris Antibodies GmbH | |||||||
| +1 additional image |
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CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN200716 | HDLBP - human gene knockout kit via CRISPR |
||
|
Human | GFP-Puro |
1 kit /
€1,200.00
|
| OriGene Technologies, Inc. | |||
| KN200716BN | HDLBP - human gene knockout kit via CRISPR |
||
|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN200716LP | HDLBP - human gene knockout kit via CRISPR |
||
|
Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN200716RB | HDLBP - human gene knockout kit via CRISPR |
||
|
Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN307635 | Hdlbp - mouse gene knockout kit via CRISPR |
||
|
Mouse | GFP-Puro |
1 kit /
€1,200.00
|
| OriGene Technologies, Inc. | |||
| KN307635BN | Hdlbp - mouse gene knockout kit via CRISPR |
||
|
Mouse | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN307635LP | Hdlbp - mouse gene knockout kit via CRISPR |
||
|
Mouse | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN307635RB | Hdlbp - mouse gene knockout kit via CRISPR |
||
|
Mouse | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP321641 | Vigilin / HDLBP (transcript variant 1) |
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| Recombinant protein of human high density lipoprotein binding protein (HDLBP), transcript variant 1 | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€680.00
|
|
| OriGene Technologies, Inc. | |||||
Human Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RC221641L2V | Lenti ORF particles, HDLBP (mGFP-tagged) - Human high density lipoprotein binding protein (HDLBP), transcript variant 1, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,320.00
|
|
| OriGene Technologies, Inc. | ||
| RC200716L2V | Lenti ORF particles, HDLBP (mGFP-tagged)-Human high density lipoprotein binding protein (HDLBP), transcript variant 2, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,320.00
|
|
| OriGene Technologies, Inc. | ||
| RC221641L1V | Lenti ORF particles, HDLBP (Myc-DDK tagged) - Human high density lipoprotein binding protein (HDLBP), transcript variant 1 , 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,180.00
|
|
| OriGene Technologies, Inc. | ||
| RC200716L1V | Lenti ORF particles, HDLBP (Myc-DDK-tagged)-Human high density lipoprotein binding protein (HDLBP), transcript variant 2, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,180.00
|
|
| OriGene Technologies, Inc. | ||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR211873L2V | Lenti ORF particles, Hdlbp (GFP-tagged) - Mouse high density lipoprotein (HDL) binding protein (Hdlbp), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€2,130.00
|
|
| OriGene Technologies, Inc. | ||
