Vimentin antibody

Principal name

Vimentin antibody

Alternative names for Vimentin antibody

VIM

SwissProt ID

P02543 (Pig), P08670 (Human), P09654 (Chick), P20152 (Mouse), P31000 (Rat), P48616 (Bovin), P48670 (Crigr), Q92155 (Cypca), Q9MZA9 (Sheep)

Gene ID

7431 (VIM), 280955, 81818 (Vim)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Chk (Chicken), African clawed frog, Por (Porcine), Ze (Zebrafish), Sh (Sheep), Potoroo, Amph (Amphibian), Fi (Fish), Can (Canine), Gt (Goat), Hst (Hamster), Mky (Monkey), Xen (Xenopus), Rb (Rabbit), Mam (Mammalia), Fe (Feline), All Species

Available hosts

Rabbit, Mouse, Chicken, Guinea Pig, Rat, Goat

Available applications

Enzyme Immunoassay (E), Paraffin Sections (P), Western blot / Immunoblot (WB), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Frozen Sections (C), ELISA (detection) (E(detection)), Luminex (LMNX)

Background of Vimentin antibody

Vimentin is the most ubiquituos intermediate filament protein and the first to be expressed during cell differentiation. All primitive cell types express vimentin but in most non-mesenchymal cells it is replaced by other intermediate filament proteins during differentiation. Vimentin is expressed in a wide variety of mesenchymal cell types - fibroblasts, endothelial cells etc., and in a number of other cell types derived from mesoderm, e.g., mesothelium and ovarian granulosa cells. In non-vascular smooth muscle cellsand striated muscle, vimentin is often replaced by desmin, however, during regeneration, vimentin is reexpressed. Cells of the lymfo-haemopoietic system (lymphocytes, macrophages etc.) also express vimentin, sometimes in scarce amounts. Vimentin is also found in mesoderm derived epithelia, e.g. kidney (Bowman capsule), endometrium and ovary (surface epithelium), in myoepithelial cells (breast, salivary and sweat glands), an in thyroid gland epithelium. In these cell types, as in mesothelial cells, vimentin is coexpressed with cytokeratin . Furthermore, vimentin is detected in many cells from the neural crest. Particularly melanocytes express abundant vimentin. In glial cells vimentin is coexpressed with glial filament acidic protein (GFAP). Vimentin is present in many different neoplasms but is particulary expressed in those originated from mesenchymal cells. Sarcomas e.g., fibrosarcoma, malignt fibrous histiocytoma, angiosarcoma, and leio- and rhabdomyosarcoma, as well as lymphomas, malignant melanoma and schwannoma, are virtually always vimentin positive.
Mesoderm derived carcinomas like renal cell carcinoma, adrenal cortical carcinoma and adenocarcinomas from endometrium and ovary usually express vimentin. Also thyroid carcinomas are vimentin positive. Any low differentiated carcinoma may express some vimentin. Vimentin is frequently included in the so-called primary panel (together with CD45, cytokeratin, and S-100 protein). Intense staining reaction for vimentin without coexpression of other intermediate filament proteins is strongly suggestive of a mesenchymal tumour or malignant melanoma. Gene locus: Human chomosome 10p13.

General readings

1. Lilienbaum, A.; Legagneux, V.; Portier, M.-M.; Dellagi, K.; Paulin, D. : Vimentin gene: expression in human lymphocytes and in Burkitt's lymphoma cells. EMBO J. 5: 2809-2814, 1986.
2. Mor-Vaknin, N.; Punturieri, A.; Sitwala, K.; Markovitz, D. M. : Vimentin is secreted by activated macrophages. Nature Cell Biol. 5: 59-63, 2003
3 Ferrari, S.; Cannizzaro, L. A.; Battini, R.; Huebner, K.; Baserga, R. : The gene encoding human vimentin is located on the short arm of chromosome 10. Am. J. Hum. Genet. 41: 616-626, 1987.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Vimentin antibody

Western blot of NIH 3T3 cells showing specific immunolabeling of the ~50k Vimentin protein. Chicken Ig Ig Fraction Hu, Mam, Ms, Rt C, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Vimentin antibody

Figure 1. Immunofluorescence staining of 3T3 mouse embryonal fibroblast cell line with anti-Vimentin (VI-01) Dyomics 547. Nuclei are stained with DAPI (blue). Mouse IgM VI-01 Purified All Species ICC/IF, WB
0.1 mg / €240.00
  Acris Antibodies GmbH

Vimentin antibody

Vimentin Mouse IgG1 VI-RE/1 Purified Hu E, ICC/IF, WB
0.1 mg / €240.00
  Acris Antibodies GmbH

Vimentin antibody

Figure 1. Immunofluorescence staining of chicken postnatal erythrocytes. Tubulin (red) was stained with anti-alpha-tubulin (TU-01; BM753S), vimentin (green) with anti-Vimentin (VI-10). Nuclei are stained with DAPI (blue). Mouse IgM VI-10 Purified Chk, Hu, Por, Rt ICC/IF, IP, P, WB
0.1 mg / €240.00
  Acris Antibodies GmbH

Vimentin antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-VIM mouse monoclonal antibody. (UM800054; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min) Mouse IgG1 UMAB159 Purified Hu P
0.1 ml / €379.00
  OriGene Technologies, Inc.

UltraMAB

Vimentin antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-VIM mouse monoclonal antibody. (UM800055; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min) Mouse IgG1 UMAB160 Purified Hu P
0.1 ml / €379.00
  OriGene Technologies, Inc.

UltraMAB

Vimentin antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-VIM mouse monoclonal antibody. (UM800054; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min) Mouse IgG1 UMAB159 Purified Hu P
30 µl / €140.00
  OriGene Technologies, Inc.

UltraMAB

Vimentin antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-VIM mouse monoclonal antibody. (UM800055; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min) Mouse IgG1 UMAB160 Purified Hu P
30 µl / €140.00
  OriGene Technologies, Inc.

UltraMAB

Vimentin antibody

Western blot analysis of Vimentin-S82 (Cat. #TA324728) in Hela cell line lysates (35ug/lane). Vimentin (arrow) was detected using the purified Pab. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

Vimentin antibody

VIM ELISA with 5C10 Capture (TA600392) and 7B10 Detection (TA700390) Antibodies. Substrate used: Recombinant Human VIM (TP301546) Mouse IgG1 OTI7B10 Purified Hu E, LMNX
50 µg / €345.00
  OriGene Technologies, Inc.

Vimentin antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-VIM mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801250) Mouse IgG2a OTI5D7 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Vimentin antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-VIM mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801297) Mouse IgG1 OTI1A9 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Vimentin antibody

Immunohistochemical staining of paraffin-embedded Human melanoma tissue using anti-VIM mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801319) Mouse IgG1 OTI5C10 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Vimentin antibody

Immunohistochemistry on paraffin section of human colon. Mouse IgG1 RV202 FITC Can, Chk, Gt, Hu, Mky, Ms, Por, Rt, Ze C, F, ICC/IF
1 ml / €380.00
  Acris Antibodies GmbH

Vimentin antibody

Immunohistochemistry on paraffin section of human colon. Mouse IgG1 RV202 HRP Can, Chk, Gt, Hst, Hu, Mky, Ms, Por, Rt, Ze C, F, ICC/IF
1 ml / €380.00
  Acris Antibodies GmbH

Vimentin antibody

Immunohistochemistry on Frozen section of swine colon using Vimentin Antibody Cat.-No BM6008P showing positive staining in connective tissue cells and no reactivity in epithelial cells. Mouse IgG1 RV202 Purified Can, Chk, Gt, Hst, Hu, Mky, Ms, Por, Rt, Xen, Ze C, F, ICC/IF, P, WB
0.1 mg / €370.00
  Acris Antibodies GmbH

Vimentin antibody

Immunofluorescence staining of NIH/3T3 ( mouse embryonal fibroblast cell line ) with VIM monoclonal antibody, clone VI-01 ( Cat # MAB3880 ). Nuclei are stained with DAPI ( blue ). Mouse IgM VI-01 Mam, Por ICC/IF, IP, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

Vimentin antibody

Human NT-2; Immunohistochemistry with NT-2 cell line tissue; VIM antibody - C-terminal region (ARP48226_P050) in Human NT-2 cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Bov, Chk, Hu, Ms, Por, Rt, Ze P, WB
50 µg / €350.00
  AVIVA Systems Biology

Vimentin antibody

Human melanoma stained with anti-Vimentin Antibody Cat.-No AM11201PU (Clone SP20). Rabbit SP20 Supernatant Hu F, P, WB
0.5 ml / €390.00
  Acris Antibodies GmbH

Vimentin antibody

Human melanoma stained with anti-Vimentin Antibody Cat.-No AM11201PU (Clone SP20). Rabbit SP20 Supernatant Hu F, P, WB
1 ml / €550.00
  Acris Antibodies GmbH

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