Vinculin (VCL) antibody

Principal name

Vinculin (VCL) antibody

Alternative names for Vinculin (VCL) antibody

Metavinculin

SwissProt ID

O46037 (Drome), P12003 (Chick), P18206 (Human), P26234 (Pig), P85972 (Rat), Q04615 (Xenla), Q64727 (Mouse)

Gene ID

7414 (VCL), 396422, 22330 (Vcl)

Available reactivities

Chk (Chicken), Hu (Human), Ms (Mouse), Rb (Rabbit), Rt (Rat), Bov (Bovine), Amph (Amphibian), Can (Canine), Xen (Xenopus), Tr (Turkey), Mky (Monkey), Por (Porcine), Eq (Equine), GP (Guinea Pig), Ze (Zebrafish)

Available hosts

Mouse, Rabbit

Available applications

Frozen Sections (C), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Flow Cytometry (F), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection))

Background of Vinculin (VCL) antibody

Vinculin is a cytoskeletal protein that plays an important role in the regulation of focal adhesions and embryonic development. Three structural vinculin domains include an amino-termil head, a short, flexible proline-rich region and a carboxy-termil tail. In the ictive state, the head and tail domains of vinculin interact to form a closed confirmation. The open and active form of vinculin translocates to focal adhesions where it is thought to be involved in anchoring F-actin to the membrane and regulation of cell migration. Phospholipid binding to the tail domain and subsequent phosphorylation of vinculin at Ser1033 and Ser1045 by PKC-a and Tyr100 and Tyr1065 by Src kises weakens the head-tail interaction. This change in vinculin allows the binding of a number of other proteins, including talin, a-actinin and paxillin, which disrupts the head-tail interaction and initiates the conformatiol change from the ictive to active state. Vinculin deficiencies are associated with a decrease in cell adhesion and an increase in cell motility, suggesting a possible role in metastatic growth. This is supported by a recently demonstrated relationship between decreased vinculin expression and increased carcinogenesis and metastasis in colorectal carcinoma.

General readings

1. Bakolitsa, C., Cohen, D. M., Bankston, L. A., Bobkov, A. A., Cadwell, G. W., Jennings, L., Critchley,
D. R., Craig, S. W., Liddington, R. C.Structural basis for vinculin activation at sites of cell
adhesion.Nature 430: 583-586, 2004.
2. Mulligan, L. M., Gardner, E., Telenius, H., Ponder, B. A. J.Complementary physical and genetic
techniques map the vinculin (VCL) gene on chromosome 10q.Genomics 13: 1347-1349, 1992.
3. Vasile, V. C., Edwards, W. D., Ommen, S. R., Ackerman, M. J.Obstructive hypertrophic
cardiomyopathy is associated with reduced expression of vinculin in the intercalated disc.Biochem.
Biophys. Res. Commun. 349: 709-715, 2006.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Vinculin (VCL) antibody

Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Vinculin (VCL) antibody

Immunofluorescence: Vinculin Antibody (hVIN-1) [NB600-1293] - A computer-generated micrograph showing the superimposed immunofluorescent staining of chicken lens cells using FITC-phalloidin (green), Monoclonal Anti-Human Vinculin, clone No. hVIN-1  (red) and Rabbit Anti-Pan Cadherin Mouse IgG1 hVIN-1 Ascites Amph, Bov, Can, Chk, Hu, Ms, Rt, Tr, Xen C, ICC/IF, P, WB
0.1 ml / €380.00
  Novus Biologicals Inc.

Vinculin (VCL) (C-term) antibody

Human heart stained with anti-Vinculin antibody Rabbit IgG SP117 Purified Hu P
0.5 ml / €510.00
  Acris Antibodies GmbH

Vinculin (VCL) (C-term) antibody

Human heart stained with anti-Vinculin antibody Rabbit IgG SP117 Purified Hu P
1 ml / €730.00
  Acris Antibodies GmbH

Vinculin (VCL) (C-term) antibody

Human heart stained with anti-Vinculin antibody Rabbit IgG SP117 Purified Hu P
0.1 ml / €290.00
  Acris Antibodies GmbH

Vinculin (VCL) (C-term) antibody

AP23808PU Vinculin antibody staining of Human Pancreas. Rabbit Purified Hu P
0.5 ml / €360.00
  Acris Antibodies GmbH

Vinculin (VCL) (C-term) antibody

AP23808PU Vinculin antibody staining of Human Pancreas. Rabbit Purified Hu P
1 ml / €520.00
  Acris Antibodies GmbH

Vinculin (VCL) (C-term) antibody

AP23808PU Vinculin antibody staining of Human Pancreas. Rabbit Purified Hu P
0.1 ml / €190.00
  Acris Antibodies GmbH

Vinculin (VCL) antibody

Predicted band size: 123 kDa . Positive control: Hela cell lysate. Recommended dilution: 1/1000-4000. (Gel: 8%SDS-PAGE Lysate: 40 ug Primary antibody: 1/1200 dilution Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Vinculin (VCL) antibody

Western blot analysis of Vinculin in PC-3 cell lysate with Vinculin antibody at (A) 1 and (B) 2 ug/ml. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Vinculin (VCL) antibody

Western blot analysis of extracts of various cell lines, using VCL antibody. Rabbit IgG Purified Hu, Ms, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Vinculin (VCL) antibody

Vinculin (VCL) Rabbit IgG Purified Hu, Ms, Rt P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Vinculin (VCL) antibody

WB Suggested Anti-VCL Antibody; Titration: 1.0 ug/ml; Positive Control: HepG2 Whole Cell Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

Vinculin (VCL) antibody

Western blot analysis of vinculin expression in HepG2 Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Vinculin (VCL) antibody

Western blot analysis of Vinculin in 293, A431, A549, HeLa, HepG2, K562, 3T3, Raji, U937 cell lysate and rat heart, mouse lung, rat lung, mouse spleen, rat spleen, rabbit spleen, mouse brain, rabbit brain and chicken spleen tissue lysate with Vinculin ant Mouse IgG 7E10 Purified Chk, Hu, Ms, Rb, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Vinculin (VCL) antibody

Western blot analysis of Vinculin in A431, Daudi, Jurkat, Raji, and THP-1 cell lysate with Vinculin antibody at 1 ug/mL. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Vinculin (VCL) antibody

Vinculin (VCL) Mouse IgG1 VIN-54 Purified Chk, Hu, Ms, Rb, Rt C, ICC/IF, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

Vinculin (VCL) antibody

Vinculin (VCL) Mouse IgG1 FB11 Purified Hu C, E, WB
0.1 mg / €470.00
  Acris Antibodies GmbH

Vinculin (VCL) antibody

Immunohistochemistry (IHC) analyzes of Vinculin antibody (Cat.-No.: AP06605PU-N) in paraffin-embedded Human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Vinculin (VCL) (N-term) antibody

Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with VINC antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

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