Visfatin / NAMPT antibody

Principal name

Visfatin / NAMPT antibody

Alternative names for Visfatin / NAMPT antibody

NAmPRTase, PBEF, PBEF1, Nicotinamide phosphoribosyltransferase, Pre-B-cell colony-enhancing factor 1

SwissProt ID

P43490 (Human), Q52I78 (Pig), Q80Z29 (Rat), Q99KQ4 (Mouse)

Gene ID

10135 (NAMPT), 59027 (Nampt), 297508

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Ze (Zebrafish), Can (Canine), Chk (Chicken), Por (Porcine), African clawed frog, Xen (Xenopus), Prim (Primate)

Available hosts

Rabbit, Mouse, Sheep, Rat

Available applications

Enzyme Immunoassay (E), Immunoprecipitation (IP), Paraffin Sections (P), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Frozen Sections (C)

Background of Visfatin / NAMPT antibody

Visfatin, a newly identified adipocytokine ,which is predominantly secreted from visceral adipose tissue both in humans and mice . Visfatin corresponds to a protein identified previously as pre-B cell colony-enhancing factor (PBEF). Visfatin exerted insulin-mimetic effects in cultured cells, various insulin-sensitive tissues such as liver, muscle, and fat ,and lowered plasma glucose levels in mice. Mice heterozygous for a targeted mutation in the visfatin gene had modestly higher levels of plasma glucose relative to wild-type littermates. visfatin binds to and activates the insulin receptor. Which may lead to new insights into glucose homeostasis and/or new therapies for metabolic disorders such as diabetes.
Excess adiposity is the most important risk in the development of insulin resistance and type 2 diabetes mellitus (T2DM). Adipose tissue produces several proteins (adipocytokines) such as leptin, adiponectin, resistin, tumor necrosis factor-alpha, and IL-6, that modulate insulin sensitivity and appear to play an important role in the pathogenesis of insulin resistance, diabetes, dyslipidemia, inflammation, and atherosclerosis. However, the mechanisms by which fat tissue induces insulin resistance and the role of adipocytokines in the pathogenesis of T2DM have not been well established. Visfatin, also known as pre-B cell colony-enhancing factor (PBEF), is a cytokine that is highly expressed in visceral fat and was originally isolated as a secreted factor that synergizes with IL-7 and stem cell factors to promote the growth of B cell precursors. Visfatin homologs have been identified in carp, invertebrate mollusks , and bacteria, as well as in vertebrates, including humans and the mouse. It has been postulated to play a role in innate immunity.
Visfatin exerts insulin-mimetic effects that are dose-dependent and quantitatively similar to those of insulin in stimulating muscle and adipocyte glucose transport, and in inhibiting hepatocyte glucose production. Intravenous injection of recombinant visfatin in mice decreased plasma glucose in a dose-dependent fashion. In keeping with its insulin-mimetic effects, visfatin was as effective as insulin in reducing hyperglycemia in insulindeficient diabetic mice. Visfatin was also found to be bound to and activate insulin receptor, causing receptor phosphorylation and the activation of downstream signaling molecules. However, visfatin and insulin did not compete for binding to the insulin receptor, indicating that the two proteins were recognized by different regions of the receptor. Thus, visfatin might play a role in glucose homeostasis and dysregulation in biosynthesis or signal transduction, and might contribute to the pathogenesis of diabetes.

General readings

Stephens JM., et al. (2006) Curr Opin Lipidol. 17:128-31.
Hug C, Lodish HF , et al (2005) Science. 307:36-97.

  1. Quick-Filters:
  2. Highly Validated (1)
  3. Overnight Delivery (1)

Items 1 to 20 of 54 total

per page
Page:
  1. 1
  2. 2
  3. 3

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Visfatin / NAMPT antibody

NAMPT is expressed in distinct spleen cell populations (CD11c positive and negative). Immunohistochemistry of mouse spleen frozen section (14A5: 10 μg /ml). Secondary Ab: anti-Rat-Alexa488, anti-CD11c-Alexa568 Rat IgG2a 14A5 Purified Hu, Ms C, E, ICC/IF, IP, WB
0.1 mg / €300.00
  Acris Antibodies GmbH

Visfatin / NAMPT antibody

Western blot analysis using recombinant human Visfatin. Rabbit Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Visfatin / NAMPT antibody

Western blot analysis using Rat Visfatin recombinant rat Visfatin. Lane 1: 50 ng, Lane 2: 250 ng, Lane 3: 1 ug. Rabbit Purified Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Visfatin / NAMPT antibody

Predicted band size: 56 kDa . Positive control: A375 cell and mouse liver tissue lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lane1: A375 cell lysate Lane2: Mouse liver tissue lysate Lysates: 40 ug per lane Primary antibody: 1/350 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 minutes) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Visfatin / NAMPT antibody

Predicted band size: 56 kDa . Positive control: A375, 293T cell and mouse liver tissue, Jurkat cell and human fetal kidney tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: A375 cell lysate Lane2: 293T cell lysate Lane3: Mouse liver tissue lysate Lane4: Jurkat cell lysate Lane5: Human fetal kidney tissue lysate Lysates: 40 ug per lane Primary antibody: 1/700 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Visfatin / NAMPT antibody

Western blot analysis of extracts of various cell lines, using NAMPT antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

Visfatin / NAMPT antibody

To detect Human Visfatin by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Human Visfatin. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Visfatin / NAMPT antibody

To detect Human Visfatin by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human Visfatin (TA328472) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human Visfatin. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Visfatin / NAMPT antibody

Western Blot: NAMPT Antibody [TA336957] - Western blot analysis of NAMPT in skeletal muscle lysate. Lane 1 shows pre-immune sera. Lane 2 shows human, Lane 3 shows mouse and Lane 4 shows rat skeletal muscle using TA336957 at 1:5000 dilution. Rabbit Purified Chk, Hu, Ms, Prim, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Visfatin / NAMPT antibody

Western blot analysis using PBEF1 antibody Cat.-No AM06217SU-N against truncated PBEF1-His recombinant protein (Lane 1) and full-length GFP-PBEF1(aa1-491) transfected COS7 cell lysate (Lane 2). Mouse IgG1 1D3A12 Ascites Hu E, WB
0.1 ml / €360.00
  Acris Antibodies GmbH

Visfatin / NAMPT antibody

Western blot analysis: Cell lysates of 293T (30 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Visfatin (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG1 4D5 Purified Hu E, ICC/IF, P, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

Visfatin / NAMPT antibody

Western blot analysis: Cell lysates of 293T (30 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Visfatin (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG1 4D5 Purified Hu E, ICC/IF, P, WB
50 µl / €230.00
  Acris Antibodies GmbH

Visfatin / NAMPT antibody

Visfatin / NAMPT Rabbit Biotin Hu E, WB
50 µg / €340.00
  Acris Antibodies GmbH

Visfatin / NAMPT antibody

Visfatin / NAMPT Rabbit Biotin Hu E, WB
25 µg / €230.00
  Acris Antibodies GmbH

Visfatin / NAMPT antibody

Visfatin / NAMPT Rabbit Aff - Purified Hu E, P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

Visfatin / NAMPT antibody

Visfatin / NAMPT Rabbit Aff - Purified Hu E, P, WB
50 µg / €230.00
  Acris Antibodies GmbH

Visfatin / NAMPT antibody

Western Blot analysis of NAMPT expression in transfected 293T cell line (H00010135-T03) by NAMPT MaxPab polyclonal antibody.

Lane 1: PBEF1 transfected lysate(37.60 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

Visfatin / NAMPT antibody

Western blot analysis of cell lysates of 293T ( 30 µg ) were resolved by SDS - PAGE , transferred to NC membrane and probed with NAMPT monoclonal antibody , clone 4D5 ( 1 : 1000 ) ( Cat # MAB1122 ) . Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG1 4D5 Hu E, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

Visfatin / NAMPT antibody

Western blot analysis using NAMPT monoclonal antibody, clone 4E11C9 ( Cat # MAB5553 ) against truncated NAMPT recombinant protein. Mouse IgG1 4E11C9 Hu E, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

Visfatin / NAMPT antibody

Visfatin / NAMPT Sheep Hu E
1 ml / €560.00
  Abnova Taiwan Corp.

Items 1 to 20 of 54 total

per page
Page:
  1. 1
  2. 2
  3. 3
  • LinkedIn