Visinin-like protein 1 / HLP3 antibody
Principal name
Visinin-like protein 1 / HLP3 antibody
Alternative names for Visinin-like protein 1 / HLP3 antibody
VILIP, VLP-1, VSNL1, VISL1, Neural visinin-like protein 1, NVL-1, NVP-1, Hippocalcin-like protein 3
Ncbi ID
9606, 5874, NP_003376, NP_003376.2, NM_003385, NP_036168.1, NM_012038
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of Visinin-like protein 1 / HLP3 antibody
Visinin was origilly isolated biochemically from chicken reti as a major protein of about 24 kDa on SDS-PAGE. Following cloning and sequencing of visinin, several visinin-like proteins were discovered by homology screening. One of these, visinin-like 1 is a small calcium binding protein which is very abundant in the nervous system and is found only in neurons, though different neurons have different levels of expression. It is particularly concentrated in cerebellar Purkinje cells, and tends to be most abundant in perikarya and dendrites. The protein was discovered independently by several groups and is therefore also sometimes known as hippocalcin-like protein 3, HLP3, HPCAL3, HUVISL1, VLP-1, VILIP and VILIP-1. The protein belongs to the large superfamily of calmodulin and paravalbumin type proteins which function by binding calcium ions. Calcium binding alters the conformation of these proteins and allow them to interact with other binding partners, the properties of which they may alter. Visinin-like 1 has four "EF hand" domains, which are negatively charged helix-turn-helix peptides that are responsible for calcium binding. Visinin-like 1 is 191 amino acids in size and has a molecular weight on SDS-PAGE of ~22kDa. The protein has recently been suggested to be a useful biomarker of Alzheimer's disease and traumatic brain injury.
Western Blot analysis of VSNL1 expression in transfected 293T cell line by VSNL1 monoclonal antibody (M01), clone 2F1-E3.Lane 1: VSNL1 transfected lysate(22.1 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged VSNL1 is approximately 0.3ng/ml as a capture antibody.
Formalin-fixed and paraffin-embedded human brain tissue reacted with VSNL1 polyclonal antibody ( Cat # PAB3604 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with VSNL1 polyclonal antibody ( Cat # PAB3604 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
The VSNL1 polyclonal antibody ( Cat # PAB3604 ) is used in Western blot to detect VILIP1 in mouse brain tissue lysate.
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with VILIP1 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human brain tissue reacted with VILIP1 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
The anti-VILIP1 (AP11591PU-N) is used in Western blot to detect VILIP1 in mouse brain tissue lysate.
Recombinant human VILIP-1, 1-191 aa: 15% SDS-PAGE (3 µg)
VSNL1 Antibody (Center) (AP54522PU-N)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of VSNL1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Western blot analysis of VSNL1 (arrow) using rabbit polyclonal VSNL1 Antibody (Center) (AP54522PU-N). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the VSNL1 gene.
VSNL1 Antibody (Center) (AP54522PU-N) western blot analysis in mouse heart tissue lysates (35 µg/lane). This demonstrates the VSNL1 antibody detected the VSNL1 protein (arrow).
Confocal image of adult rat cerebellar cortex stained with VSNL1 (green) and chicken polyclonal antibody to MAP2 (red) and DNA (blue). VSNL1 antibody reveals synapses in the molecular layer (ML) strongly. Synaptic regions are also seen in the granule cell layer (GC). The perikarya of Purkinje cells (PC) are revealed with the MAP2 antibody (4). Little staining is seen in the white matter (WM). Protocol on Data-sheet.
Western blot of bovine cerebellum homogenate stained with VSNL1. Note the strong clean band running at 22kDa. Protocol on Data-sheet.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VSNL1 (RC205337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VSNL1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VSNL1 (RC205337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VSNL1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VSNL1 (RC205337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VSNL1.
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-VSNL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801896)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-VSNL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801896)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-VSNL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801896)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VSNL1 (RC205337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VSNL1.
Immunohistochemical staining of paraffin-embedded Human adult brain tissue using anti-VSNL1 mouse monoclonal antibody. (TA801897) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Human embryonic brain cortex tissue using anti-VSNL1 mouse monoclonal antibody. (TA801897) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum using anti-VSNL1 mouse monoclonal antibody. (TA801897) Dilution: 1:150
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VSNL1 (RC205337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VSNL1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VSNL1 (RC205337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VSNL1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VSNL1 (RC205337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VSNL1.
Immunohistochemical staining of paraffin-embedded Human adult brain tissue using anti-VSNL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801900)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VSNL1 (RC205337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VSNL1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VSNL1 (RC205337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VSNL1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VSNL1 (RC205337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VSNL1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VSNL1 (RC205337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VSNL1.
Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum using anti-VSNL1 mouse monoclonal antibody. (TA801904) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Human embryonic brain cortex tissue using anti-VSNL1 mouse monoclonal antibody. (TA801904) Dilution: 1:150
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VSNL1 (RC205337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VSNL1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VSNL1 (RC205337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VSNL1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VSNL1 (RC205337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VSNL1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VSNL1 (RC205337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VSNL1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VSNL1 (RC205337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VSNL1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VSNL1 (RC205337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VSNL1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VSNL1 (RC205337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VSNL1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VSNL1 (RC205337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VSNL1.
Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum using anti-VSNL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801992)
Immunohistochemical staining of paraffin-embedded Human adult brain tissue using anti-VSNL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801992)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VSNL1 (RC205337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VSNL1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VSNL1 (RC205337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VSNL1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VSNL1 (RC205337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VSNL1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VSNL1 (RC205337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VSNL1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VSNL1 (RC205337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VSNL1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VSNL1 (RC205337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VSNL1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VSNL1 (RC205337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VSNL1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VSNL1 (RC205337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VSNL1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VSNL1 (RC205337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VSNL1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VSNL1 (RC205337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VSNL1.
Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum using anti-VSNL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802028)
Immunohistochemical staining of paraffin-embedded Human embryonic brain cortex tissue using anti-VSNL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802028)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VSNL1 (RC205337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VSNL1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VSNL1 (RC205337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VSNL1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VSNL1 (RC205337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VSNL1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VSNL1 (RC205337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VSNL1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VSNL1 (RC205337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VSNL1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VSNL1 (RC205337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VSNL1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VSNL1 (RC205337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VSNL1.
Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum using anti-VSNL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802072)
Immunohistochemical staining of paraffin-embedded Human embryonic brain cortex tissue using anti-VSNL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802072)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VSNL1 (RC205337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VSNL1.
Immunohistochemical staining of paraffin-embedded Human embryonic brain cortex tissue using anti-VSNL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802073)
Immunohistochemical staining of paraffin-embedded Human adult brain tissue using anti-VSNL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802073)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VSNL1 (RC205337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VSNL1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VSNL1 (RC205337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VSNL1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VSNL1 (RC205337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VSNL1.
Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum using anti-VSNL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802088)
Immunohistochemical staining of paraffin-embedded Human embryonic brain cortex tissue using anti-VSNL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802088)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VSNL1 (RC205337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VSNL1.
Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum using anti-VSNL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802089)
Immunohistochemical staining of paraffin-embedded Human embryonic brain cortex tissue using anti-VSNL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802089)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VSNL1 (RC205337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VSNL1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VSNL1 (RC205337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VSNL1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VSNL1 (RC205337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VSNL1.
Immunohistochemical staining of paraffin-embedded Human adult heart tissue using anti-VSNL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802092)
Immunohistochemical staining of paraffin-embedded Human embryonic brain cortex tissue using anti-VSNL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802092)
Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum using anti-VSNL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802092)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VSNL1 (RC205337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VSNL1.
Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum using anti-VSNL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802102)
Immunohistochemical staining of paraffin-embedded Human adult brain tissue using anti-VSNL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802102)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VSNL1 (RC205337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VSNL1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VSNL1 (RC205337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VSNL1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VSNL1 (RC205337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VSNL1.
Immunohistochemical staining of paraffin-embedded Human adult brain tissue using anti-VSNL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802256)
Immunohistochemical staining of paraffin-embedded Human embryonic brain cortex tissue using anti-VSNL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802256)
Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum using anti-VSNL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802256)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VSNL1 (RC205337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VSNL1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VSNL1 (RC205337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VSNL1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VSNL1 (RC205337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VSNL1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VSNL1 (RC205337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VSNL1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VSNL1 (RC205337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VSNL1.
Predicted band size: 22 kDa . Positive control: Hela cell lysate. Recommended dilution: 1/1000-3000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/900 dilution Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution Exposure time: 5 seconds)
Predicted cell location: Cytoplasm. Positive control: Human esophagus cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using VSNL1 antibody at dilution 1/25, on the right is treated with the synthetic peptide. (Original magnification:x200)
Western Blot: visinin-like 1 Antibody (2D11) [TA337119] - Bovine cerebellum homogenate stained with TA337119 in lane 10. Note the strong clean band running at 22kDa. Lane 11 shows the same material stained with another antibody to visinin-like 1, NBP2-251
Immunocytochemistry/Immunofluorescence: visinin-like 1 Antibody (2D11) [TA337119] - Confocal image of adult rat cerebellar cortex stained with TA337119 (green), chicken polyclonal antibody to MAP2 NB300-213 (red) and DNA (blue). TA337119 reveals synapses
Western Blot: visinin-like 1 Antibody (3A9) [TA337120] - Bovine cerebellum homogenate stained with TA337120 in lane 11. Note the strong clean band running at 22 kDa. Lane 10 shows the same material stained with another antibody to visinin-like 1, NBP2-251
Immunocytochemistry/Immunofluorescence: visinin-like 1 Antibody (3A9) [TA337120] - Confocal image of adult rat cerebellum stained with TA337120 (green), chicken polyclonal antibody to MAP2 NB300-213 (red) and DNA (blue). TA337120 reveals perikarya and syn
Western Blot: visinin-like 1 Antibody [TA337121] - Western blot of rat brain homogenate. Note the strong clean band running at 18 kDa.
Immunocytochemistry/Immunofluorescence: visinin-like 1 Antibody [TA337121] - Confocal image of adult rat cerebellar cortex stained with TA337121 (green), polyclonal antibody to NF-M: NB300-133 (red) and DNA (blue). TA337121 reveals synapses in the molecu
Western Blot: visinin-like 1 Antibody [TA337122] - Bovine cerebellum homogenate. Note the strong clean band running at 22 kDa.
Immunocytochemistry/Immunofluorescence: visinin-like 1 Antibody [TA337122] - Neuron-glia cell culture stained with TA337122 (red), monoclonal antibody to GFAP: NBP1-05197 (green) and DNA (blue). TA337122 reveals strong staining throughout some small neuro
- Quick-Filters:
- Highly Validated (8)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| UM800034 | Visinin-like protein 1 / HLP3 antibody |
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| VSNL1 mouse monoclonal antibody,clone UMAB115 | |||||||
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Mouse | IgG1 | UMAB115 | Purified | Hu | P |
0.1 ml /
€468.00
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| OriGene Technologies, Inc. | |||||||
Highly validated antibody!
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| UM800035 | Visinin-like protein 1 / HLP3 antibody |
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| VSNL1 mouse monoclonal antibody,clone UMAB116 | |||||||
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Mouse | IgG1 | UMAB116 | Purified | Hu | P |
0.1 ml /
€468.00
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| OriGene Technologies, Inc. | |||||||
Highly validated antibody!
|
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| UM870034 | Visinin-like protein 1 / HLP3 antibody |
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| VSNL1 mouse monoclonal antibody,clone UMAB115 | |||||||
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Mouse | IgG1 | UMAB115 | Purified | Hu | P |
30 µl /
€120.00
|
| OriGene Technologies, Inc. | |||||||
Highly validated antibody!
|
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| UM870035 | Visinin-like protein 1 / HLP3 antibody |
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| VSNL1 mouse monoclonal antibody,clone UMAB116 | |||||||
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Mouse | IgG1 | UMAB116 | Purified | Hu | P |
30 µl /
€120.00
|
| OriGene Technologies, Inc. | |||||||
Highly validated antibody!
|
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| TA801896 | Visinin-like protein 1 / HLP3 antibody |
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| VSNL1 mouse monoclonal antibody, clone OTIF7 (formerly F7) | |||||||
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Mouse | IgG1 | OTIF7 | Purified | Hu | P, WB |
0.1 ml /
€350.00
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| OriGene Technologies, Inc. | |||||||
 +3 additional images |
Highly validated antibody!
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| TA801897 | Visinin-like protein 1 / HLP3 antibody |
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| VSNL1 mouse monoclonal antibody, clone OTI4A6 (formerly 4A6) | |||||||
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Mouse | IgG1 | OTI4A6 | Purified | Hu | P, WB |
0.1 ml /
€350.00
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| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
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| TA802092 | Visinin-like protein 1 / HLP3 antibody |
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| VSNL1 mouse monoclonal antibody, clone OTIE12 (formerly E12) | |||||||
 |
Mouse | IgG1 | OTIE12 | Purified | Hu | P, WB |
0.1 ml /
€350.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
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| TA802256 | Visinin-like protein 1 / HLP3 antibody |
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| VSNL1 mouse monoclonal antibody, clone OTIG10 (formerly G10) | |||||||
 |
Mouse | IgG2b | OTIG10 | Purified | Hu | P, WB |
0.1 ml /
€350.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
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| TA322856 | Visinin-like protein 1 / HLP3 antibody |
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| Anti-VSNL1 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA337119 | Visinin-like protein 1 / HLP3 antibody |
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| Mouse Monoclonal visinin-like 1 Antibody (2D11) | |||||||
![Western Blot: visinin-like 1 Antibody (2D11) [TA337119] - Bovine cerebellum homogenate stained with TA337119 in lane 10. Note the strong clean band running at 22kDa. Lane 11 shows the same material stained with another antibody to visinin-like 1, NBP2-251](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/NBP2-25166-1-W.jpg) |
Mouse | IgG1 | 2D11 | Purified | Bov, Hu, Ms, Rt | ICC/IF, WB |
0.1 ml /
€393.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA337120 | Visinin-like protein 1 / HLP3 antibody |
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| Mouse Monoclonal visinin-like 1 Antibody (3A9) | |||||||
![Western Blot: visinin-like 1 Antibody (3A9) [TA337120] - Bovine cerebellum homogenate stained with TA337120 in lane 11. Note the strong clean band running at 22 kDa. Lane 10 shows the same material stained with another antibody to visinin-like 1, NBP2-251](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/NBP2-25167-1-W.jpg) |
Mouse | IgG1 | 3A9 | Purified | Bov, Hu, Ms, Rt | ICC/IF, WB |
0.1 ml /
€393.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA337121 | Visinin-like protein 1 / HLP3 antibody |
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| Chicken Polyclonal visinin-like 1 Antibody | |||||||
![Western Blot: visinin-like 1 Antibody [TA337121] - Western blot of rat brain homogenate. Note the strong clean band running at 18 kDa.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/NBP2-25168-1-W.jpg) |
Chicken | Purified | Bov, Hu, Ms, Rt | ICC/IF, WB |
0.1 ml /
€393.00
|
||
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA337122 | Visinin-like protein 1 / HLP3 antibody |
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| Rabbit Polyclonal visinin-like 1 Antibody | |||||||
![Western Blot: visinin-like 1 Antibody [TA337122] - Bovine cerebellum homogenate. Note the strong clean band running at 22 kDa.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/NBP2-25169-1-W.jpg) |
Rabbit | Purified | Bov, Hu, Ms, Rt | ICC/IF, WB |
0.1 ml /
€393.00
|
||
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA801882 | Visinin-like protein 1 / HLP3 antibody |
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| VSNL1 mouse monoclonal antibody, clone OTID2 (formerly D2) | |||||||
 |
Mouse | IgG1 | OTID2 | Purified | Hu | WB |
0.1 ml /
€350.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA801883 | Visinin-like protein 1 / HLP3 antibody |
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| VSNL1 mouse monoclonal antibody, clone OTI2F7 (formerly 2F7) | |||||||
 |
Mouse | IgG1 | OTI2F7 | Purified | Hu | WB |
0.1 ml /
€350.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA801895 | Visinin-like protein 1 / HLP3 antibody |
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| VSNL1 mouse monoclonal antibody, clone OTI2E6G5 (formerly 2E6G5) | |||||||
 |
Mouse | IgG1 | OTI2E6G5 | Purified | Hu | WB |
0.1 ml /
€350.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA801898 | Visinin-like protein 1 / HLP3 antibody |
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| VSNL1 mouse monoclonal antibody, clone OTI4G10 (formerly 4G10) | |||||||
 |
Mouse | IgG1 | OTI4G10 | Purified | Hu | WB |
0.1 ml /
€350.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA801899 | Visinin-like protein 1 / HLP3 antibody |
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| VSNL1 mouse monoclonal antibody, clone OTI5C3 (formerly 5C3) | |||||||
 |
Mouse | IgG2b | OTI5C3 | Purified | Hu | WB |
0.1 ml /
€350.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA801900 | Visinin-like protein 1 / HLP3 antibody |
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| VSNL1 mouse monoclonal antibody, clone OTI2E5 (formerly 2E5) | |||||||
 |
Mouse | IgG1 | OTI2E5 | Purified | Hu | P, WB |
0.1 ml /
€350.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
||||||
| TA801901 | Visinin-like protein 1 / HLP3 antibody |
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| VSNL1 mouse monoclonal antibody, clone OTI2B4 (formerly 2B4) | |||||||
 |
Mouse | IgG1 | OTI2B4 | Purified | Hu | WB |
0.1 ml /
€350.00
|
| OriGene Technologies, Inc. | |||||||
|
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