Visinin-like protein 1 / HLP3 antibody

Principal name

Visinin-like protein 1 / HLP3 antibody

Alternative names for Visinin-like protein 1 / HLP3 antibody

VILIP, VLP-1, VSNL1, VISL1, Neural visinin-like protein 1, NVL-1, NVP-1, Hippocalcin-like protein 3

SwissProt ID

P62760 (Human), P62761 (Mouse), P62762 (Rat), P62764 (Chick)

Gene ID

7447 (VSNL1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine)

Available hosts

Mouse, Rabbit, Chicken

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Frozen Sections (C), ELISA (detection) (E(detection))

Background of Visinin-like protein 1 / HLP3 antibody

Visinin was origilly isolated biochemically from chicken reti as a major protein of about 24 kDa on SDS-PAGE. Following cloning and sequencing of visinin, several visinin-like proteins were discovered by homology screening. One of these, visinin-like 1 is a small calcium binding protein which is very abundant in the nervous system and is found only in neurons, though different neurons have different levels of expression. It is particularly concentrated in cerebellar Purkinje cells, and tends to be most abundant in perikarya and dendrites. The protein was discovered independently by several groups and is therefore also sometimes known as hippocalcin-like protein 3, HLP3, HPCAL3, HUVISL1, VLP-1, VILIP and VILIP-1. The protein belongs to the large superfamily of calmodulin and paravalbumin type proteins which function by binding calcium ions. Calcium binding alters the conformation of these proteins and allow them to interact with other binding partners, the properties of which they may alter. Visinin-like 1 has four "EF hand" domains, which are negatively charged helix-turn-helix peptides that are responsible for calcium binding. Visinin-like 1 is 191 amino acids in size and has a molecular weight on SDS-PAGE of ~22kDa. The protein has recently been suggested to be a useful biomarker of Alzheimer's disease and traumatic brain injury.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Visinin-like protein 1 / HLP3 antibody

Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum using anti-VSNL1 mouse monoclonal antibody. (UM800034; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min) Mouse IgG1 UMAB115 Purified Hu P
0.1 ml / €468.00
  OriGene Technologies, Inc.

UltraMAB

Visinin-like protein 1 / HLP3 antibody

Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum using anti-VSNL1 mouse monoclonal antibody. (UM800035; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min) Mouse IgG1 UMAB116 Purified Hu P
0.1 ml / €468.00
  OriGene Technologies, Inc.

UltraMAB

Visinin-like protein 1 / HLP3 antibody

Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum using anti-VSNL1 mouse monoclonal antibody. (UM800034; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min) Mouse IgG1 UMAB115 Purified Hu P
30 µl / €140.00
  OriGene Technologies, Inc.

UltraMAB

Visinin-like protein 1 / HLP3 antibody

Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum using anti-VSNL1 mouse monoclonal antibody. (UM800035; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min) Mouse IgG1 UMAB116 Purified Hu P
30 µl / €140.00
  OriGene Technologies, Inc.

UltraMAB

Visinin-like protein 1 / HLP3 antibody

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-VSNL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801896) Mouse IgG1 OTIF7 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Visinin-like protein 1 / HLP3 antibody

Immunohistochemical staining of paraffin-embedded Human adult brain tissue using anti-VSNL1 mouse monoclonal antibody. (TA801897) Dilution: 1:150 Mouse IgG1 OTI4A6 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Visinin-like protein 1 / HLP3 antibody

Immunohistochemical staining of paraffin-embedded Human adult heart tissue using anti-VSNL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802092) Mouse IgG1 OTIE12 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Visinin-like protein 1 / HLP3 antibody

Immunohistochemical staining of paraffin-embedded Human adult brain tissue using anti-VSNL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802256) Mouse IgG2b OTIG10 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Visinin-like protein 1 / HLP3 antibody

Predicted band size: 22 kDa . Positive control: Hela cell lysate. Recommended dilution: 1/1000-3000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/900 dilution Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution Exposure time: 5 seconds) Rabbit IgG Purified Hu, Ms, Rt P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Visinin-like protein 1 / HLP3 antibody

Western Blot: visinin-like 1 Antibody (2D11) [TA337119] - Bovine cerebellum homogenate stained with TA337119 in lane 10. Note the strong clean band running at 22kDa. Lane 11 shows the same material stained with another antibody to visinin-like 1, NBP2-251 Mouse IgG1 2D11 Purified Bov, Hu, Ms, Rt ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Visinin-like protein 1 / HLP3 antibody

Western Blot: visinin-like 1 Antibody (3A9) [TA337120] - Bovine cerebellum homogenate stained with TA337120 in lane 11. Note the strong clean band running at 22 kDa. Lane 10 shows the same material stained with another antibody to visinin-like 1, NBP2-251 Mouse IgG1 3A9 Purified Bov, Hu, Ms, Rt ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Visinin-like protein 1 / HLP3 antibody

Western Blot: visinin-like 1 Antibody [TA337121] - Western blot of rat brain homogenate. Note the strong clean band running at 18 kDa. Chicken Purified Bov, Hu, Ms, Rt ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Visinin-like protein 1 / HLP3 antibody

Western Blot: visinin-like 1 Antibody [TA337122] - Bovine cerebellum homogenate. Note the strong clean band running at 22 kDa. Rabbit Purified Bov, Hu, Ms, Rt ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Visinin-like protein 1 / HLP3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VSNL1 (RC205337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VSNL1. Mouse IgG1 OTID2 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Visinin-like protein 1 / HLP3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VSNL1 (RC205337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VSNL1. Mouse IgG1 OTI2F7 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Visinin-like protein 1 / HLP3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VSNL1 (RC205337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VSNL1. Mouse IgG1 OTI2E6G5 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Visinin-like protein 1 / HLP3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VSNL1 (RC205337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VSNL1. Mouse IgG1 OTI4G10 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Visinin-like protein 1 / HLP3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VSNL1 (RC205337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VSNL1. Mouse IgG2b OTI5C3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Visinin-like protein 1 / HLP3 antibody

Immunohistochemical staining of paraffin-embedded Human adult brain tissue using anti-VSNL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801900) Mouse IgG1 OTI2E5 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Visinin-like protein 1 / HLP3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VSNL1 (RC205337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VSNL1. Mouse IgG1 OTI2B4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

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