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Vitronectin antibody

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Principal name

Vitronectin antibody

Alternative names for Vitronectin antibody

VTN, S-protein, V75, Serum-spreading factor

SwissProt ID

P04004 (Human), P22458 (Rabit), P29788 (Mouse), P48819 (Pig)

Gene ID

22370 (Vtn), 7448 (VTN)

Ncbi ID

10090, NP_035837.1, 9606, NP_000629, NP_000629.3, NM_011707, NM_000638

Available reactivities

Ms (Mouse), Hu (Human), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Gt (Goat), Por (Porcine), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Rabbit, Mouse

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Functional assay (FN), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Flow Cytometry (F), Paraffin Sections (P)

Background of Vitronectin antibody

Vitronectin is an abundant glycoprotein (~75 kDa), consisting of 459 amino acids. About one third of the protein molecular mass is composed of carbohydrates. Vitronectin is found in blood plasma and the extracellular matrix. Vitronectin is a multifunctional protein, since it promotes attachment and spreading of animal cells in vitro, it inhibits cytolysis by the complement C5b-9 complex, and it modulates antithrombin III-thrombin action in blood coagulation.
The protein consists of three domains: the N-terminal Somatomedin B domain (1-39), a central domain with hemopexin homology (131-342) and a C-terminal domain (347-459) also with hemopexin homology. The Somatomedin B domain binds to Plasminogen Activator Inhibitor-1 (PAI-1) and is responsible for PAI-1 stabilization. Furthermore, the Somatomedin B domain can also interact with the urokinase plasminogen activator receptor (uPAR). Vitronectin-uPAR interaction is required and sufficient to initiate downstream changes in cell morphology, migration and signal transduction. High plasma levels of both PAI-1 and uPAR have been shown to correlate with a negative prognosis for cancer patients. Additionally, vitronectin is a component of platelets and is as such involved in hemostasis. Amino acid 45-47 (RGD) are capable of binding to membrane bound integrins, which serve to anchor cells to the extracellular matrix.
Vitronectin in plasma is an inactive monomer form. In contrast, tissue vitronectin is an active multimeric form and is able to interact with various matrix ligands like proteoglycans and collagen. Mice with a genetic deletion of vitronectin show delayed wound healing, suggesting an important role of vitronectin in tissue remodeling after injury.

General readings

Chain D., et al. (1991) FEBS Lett. 285: 251-256.Cherny R C., et al. (1993) J Biol Chem. 268: 9725-9729.

ARP58095_P050 - Vitronectin
Hum. Adult Placenta; Host:Rabbit. Target Name:VTN. Sample Tissue:Human Adult Placenta. Antibody Dilution: 1.0ug/ml.; VTN antibody - N-terminal region (ARP58095_P050) in Hum. Adult Placenta cells using Western Blot
ARP58095_P050 - Vitronectin
Hum. Fetal Lung; Host:Rabbit. Target Name:VTN. Sample Tissue:Human Fetal Lung. Antibody Dilution: 1.0ug/ml.; VTN antibody - N-terminal region (ARP58095_P050) in Hum. Fetal Lung cells using Western Blot
ARP58095_P050 - Vitronectin
Human HepG2; WB Suggested Anti-VTN Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: HepG2 cell lysate. VTN is supported by BioGPS gene expression data to be expressed in HepG2.; VTN antibody - N-terminal region (ARP58095_P050) in Human HepG2 cells using Western Blot
AP14110PU-N - Vitronectin
Western blot analysis of VTN antibody (C-term) in NCI-H460 cell line lysates (35ug/lane). VTN (arrow) was detected using the purified Pab.
H00007448-B01 - Vitronectin
VTN MaxPab polyclonal antibody. Western Blot analysis of VTN expression in human liver.
H00007448-B01 - Vitronectin
Western Blot analysis of VTN expression in transfected 293T cell line (H00007448-T01) by VTN MaxPab polyclonal antibody.Lane 1: VTN transfected lysate(52.58 KDa).Lane 2: Non-transfected lysate.
H00007448-B01P - Vitronectin
VTN MaxPab polyclonal antibody. Western Blot analysis of VTN expression in human liver.
H00007448-B01P - Vitronectin
Western Blot analysis of VTN expression in transfected 293T cell line (H00007448-T01) by VTN MaxPab polyclonal antibody.Lane 1: VTN transfected lysate(52.58 KDa).Lane 2: Non-transfected lysate.
H00007448-D01P - Vitronectin
Western Blot analysis of VTN expression in transfected 293T cell line (H00007448-T01) by VTN MaxPab polyclonal antibody.Lane 1: VTN transfected lysate(54.30 KDa).Lane 2: Non-transfected lysate.
MAB0808 - Vitronectin
Immunoblot analysis of human plasma protein. Lane 1 : VTN protein isolated from human plasma ( partial protein ) . Lane 2 : Human plasma ( 0.2 ul ) .
AR51561PU-N - Vitronectin
CPA2229-100ul - Vitronectin
Western blot analysis of Vitronectin expression in MCF7 (A); HepG2 (B) whole cell lysates.
TA590043 - Vitronectin
ELISA: Vitronectin Antibody - Affinity Purified
TP302929 - Vitronectin
TA321171 - Vitronectin
Predicted cell location: Cytoplasm. Positive control: Human liver and gastric cancer tissue. Recommended dilution: 1/100-300 The image on the left is immunohistochemistry of paraffin-embedded human liver cancer tissue using VTN) at dilution 1/100, on the right is treated with the synthetic peptide. (Original magnification:x 200)
TA321171 - Vitronectin
Predicted cell location: Cytoplasm. Positive control: Human liver and gastric cancer tissue. Recommended dilution: 1/100-300 The image on the left is immunohistochemistry of paraffin-embedded human gastric cancer tissue using VTN) at dilution 1/100, on the right is treated with the synthetic peptide. (Original magnification:x200)
TA324937 - Vitronectin
Western blot analysis of VTN Antibody (N-term) (Cat. #TA324937) in NCI-H460 cell line lysates (35ug/lane). VTN (arrow) was detected using the purified Pab.
TA324937 - Vitronectin
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with VTN Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
TA324937 - Vitronectin
VTN Antibody (N-term) (Cat. #TA324937) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
TA344462 - Vitronectin
Host:Rabbit; Target Name:VTN; Sample Tissue:Human Adult Placenta; Antibody Dilution: 1.0 ug/ml
TA344462 - Vitronectin
Host:Rabbit; Target Name:VTN; Sample Tissue:Human Fetal Lung; Antibody Dilution: 1.0 ug/ml
TA344462 - Vitronectin
WB Suggested Anti-VTN Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: HepG2 cell lysateVTN is supported by BioGPS gene expression data to be expressed in HepG2
TA346045 - Vitronectin
Host: Rabbit; Target Name: Vtn; Sample Tissue: Rat Kidney lysates; Antibody Dilution: 1.0 ug/ml
TA349592 - VTN
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using (VTN Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: ×200)
TA349592 - VTN
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using (VTN Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: ×200)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Vitronectin antibody
  Anti-VTN Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm. Positive control: Human liver and gastric cancer tissue. Recommended dilution: 1/100-300 The image on the left is immunohistochemistry of paraffin-embedded human liver cancer tissue using VTN) at dilution 1/100, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E
0.1 ml / €325.00
  OriGene Technologies, Inc.
+1 additional image

Vitronectin antibody
  Rabbit polyclonal VTN Antibody (N-term)  
Western blot analysis of VTN Antibody (N-term) (Cat. #TA324937) in NCI-H460 cell line lysates (35ug/lane). VTN (arrow) was detected using the purified Pab. Rabbit IgG Purified Hu F, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.
+2 additional images

Vitronectin antibody
  Rabbit Polyclonal Anti-VTN Antibody - N-terminal region  
Host:Rabbit; Target Name:VTN; Sample Tissue:Human Adult Placenta; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.
+2 additional images

Vitronectin antibody
  Rabbit Polyclonal Anti-Vtn Antibody  
Host: Rabbit; Target Name: Vtn; Sample Tissue: Rat Kidney lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Can, Eq, GP, Gt, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

Vitronectin antibody
  Rabbit Polyclonal Anti-VTN Antibody  
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using (VTN Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: ×200) Rabbit IgG Purified Hu E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.
+1 additional image

Vitronectin antibody
  Rabbit Polyclonal Vitronectin Antibody  
ELISA: Vitronectin Antibody - Affinity Purified Rabbit IgG Purified Hu E
0.1 mg / €325.00
  OriGene Technologies, Inc.

Vitronectin antibody
Vitronectin Mouse IgG1 BV2 Purified Hu C, E, IP, WB
0.1 mg / €650.00
  Acris Antibodies GmbH

Vitronectin antibody
Vitronectin Mouse IgG1 BV1 Purified Hu E, ICC/IF, IP, WB
0.1 mg / €400.00
  Acris Antibodies GmbH

Vitronectin antibody
Vitronectin Mouse IgG1 BE10 Ig Fraction Hu C, E, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

Vitronectin antibody
Vitronectin Rabbit Purified Hu E, WB
1 mg / €730.00
  Acris Antibodies GmbH

Vitronectin antibody
Vitronectin Rabbit Purified Ms C, E, WB
1 mg / €660.00
  Acris Antibodies GmbH

Vitronectin (C-term) antibody
Western blot analysis of VTN antibody (C-term) in NCI-H460 cell line lysates (35ug/lane). VTN (arrow) was detected using the purified Pab. Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Vitronectin antibody
Vitronectin Mouse IgG1 1.110 Ascites Hu E, FN, WB
25 µl / €510.00
  Acris Antibodies GmbH

Vitronectin antibody
Vitronectin Mouse IgG2a BDI215 Purified Hu E, WB
0.5 mg / €870.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

VTN - human gene knockout kit via CRISPR
VTN - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

VTN - human gene knockout kit via CRISPR
VTN - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

VTN - human gene knockout kit via CRISPR
VTN - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

VTN - human gene knockout kit via CRISPR
VTN - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Vtn - mouse gene knockout kit via CRISPR
Vtn - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Vtn - mouse gene knockout kit via CRISPR
Vtn - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

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32 products - FILTER YOUR RESULTS!

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  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
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