VKORC1 antibody

Principal name

VKORC1 antibody

Alternative names for VKORC1 antibody

VKOR, MSTP134, MSTP576, UNQ308/PRO351, Vitamin K epoxide reductase complex subunit 1, Vitamin K1 2,3-epoxide reductase subunit 1

SwissProt ID

Q6B4J2 (Bovin), Q6TEK4 (Rat), Q9BQB6 (Human), Q9CRC0 (Mouse)

Gene ID

79001 (VKORC1), 309004 (Vkorc1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit

Available applications

Western blot / Immunoblot (WB), Frozen Sections (C), Enzyme Immunoassay (E), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of VKORC1 antibody

Vitamin K is essential for blood clotting but must be enzymatically activated. vitamin K is primarily known as a cosubstrate in the posttranslational conversion of glutamate to γ-carboxyglutamate (Gla). There are at least 16 Gla-containing proteins in mammals, and eight of these are related to coagulation. This enzymatically activated form of vitamin K is a reduced form required for the carboxylation of glutamic acid residues in some blood-clotting proteins and the VKOR is the rate limiting enzyme in this pathway. The product of VKOR1 gene encodes the enzyme that is responsible for reducing vitamin K 2,3-epoxide to the enzymatically activated form. Vitamin K epoxide reductase (VKOR) is the target of warfarin, the most widely prescribed anticoagulant for thromboembolic disorders and its discovery led to better understanding of blood coagulation and development of anti-coagulant drugs. Though VKOR was discovered in 1974, the enzyme has yet to be purified and characterized fully for its activity kinetics. Positional cloning approach has made possible to identify and map the VKOR gene to human chromosome 16p12-q21. The SiRNA experiment suggest that MGC11276 messenger RNA codes for 163 amino acid warfarin sensitive with at least one transmembrane domain VKOR1 protein (1). Coumarin derived drugs such as warfarin presents a long-term treatment and prevention of thromboembolic events by inhibiting VKOR multiprotein complex. Despite extensive efforts this complex has not yet fully identified. The VKOR complex recycle vitamin K 2,3-epoxide to vitamin K hydroquinone, a cofactor that is essential for the post translational gamma-carboxylation of several blood coagulation factors. The VKOR complex is implicated in at least 2 heritable human conditions, combined deficiency of vitamin-K-dependent clotting factors type 2 (VKCFD2; Online Mendelian Inheritance in Man (OMIM) 607473), and resistance to coumarin-type anticoagulant drugs (warfarin resistance, WR; OMIM 122700).
The vitamin K epoxide reductase complex subunit 1 (VKORC1), which encodes a small transmembrane protein of the endoplasmic reticulum. The VKORC1 contains missense mutations in both human disorders and in a warfarin-resistant rat strain. Overexpression of wild-type VKORC1, but not VKORC1 carrying the VKCFD2 mutation, leads to a marked increase in VKOR activity, which is sensitive to warfarin inhibition (3). Atleast two pseudo genes for VKORC1 have been identified on chromosome 1 and X along with several alternatively spliced transcripts encoding different isoforms. Recently a recombinant VKORC1 was purified from virally infected SF9 cells and VKOR activity was preserved in this highly purified fraction (4). The VKOR1 is associated with microsomal membrane preparation. VKORC1 is a 163 amino acid protein, the recombinant VKORC1 was purified as a 21kDa protein with tag protein from Sf9 cells.

General readings

Oldenburg J, Bevans CG, Muller CR, et al. Vitamin K epoxide reductase complex subunit 1 (VKORC1): the key protein of the vitamin K cycle. Antioxid. Redox Signal. 2006; 8:347-53.

11 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

VKORC1 (N-term) antibody

Western blot analysis of VKORC1 in A549 cell lysate with VKORC1 antibody at 1 ug/mL. Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

VKORC1 antibody

WB Suggested Anti-VKORC1 Antibody; Titration: 1.0 ug/ml; Positive Control: Fetal Brain Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

VKORC1 control peptide

VKORC1
0.1 mg / €250.00
  Acris Antibodies GmbH

VKORC1 (C-term) antibody

VKORC1 Rabbit IgG Aff - Purified Hu, Ms, Rt C, E, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

VKORC1 (N-term) Control Peptide

VKORC1
50 µg / €160.00
  Acris Antibodies GmbH

VKORC1 antibody

Immunohistochemistry-Paraffin: VKORC1 Antibody [NBP1-92573] Immunohistochemical staining of human stomach, upper shows strong cytoplasmic positivity in glandular cells. Rabbit IgG Aff - Purified Hu P
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

VKORC1 (transcript variant 2)

VKORC1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

VKORC1

VKORC1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

VKORC1

VKORC1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

VKORC1 overexpression lysate

VKORC1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

VKORC1 overexpression lysate

VKORC1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

11 Item(s)

per page
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