VKORC1 antibody

Principal name

VKORC1 antibody

Alternative names for VKORC1 antibody

VKOR, MSTP134, MSTP576, UNQ308/PRO351, Vitamin K epoxide reductase complex subunit 1, Vitamin K1 2,3-epoxide reductase subunit 1

SwissProt ID

Q6B4J2 (Bovin), Q6TEK4 (Rat), Q9BQB6 (Human), Q9CRC0 (Mouse)

Gene ID

79001 (VKORC1), 309004 (Vkorc1), 27973

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of VKORC1 antibody

Vitamin K is essential for blood clotting but must be enzymatically activated. vitamin K is primarily known as a cosubstrate in the posttranslational conversion of glutamate to γ-carboxyglutamate (Gla). There are at least 16 Gla-containing proteins in mammals, and eight of these are related to coagulation. This enzymatically activated form of vitamin K is a reduced form required for the carboxylation of glutamic acid residues in some blood-clotting proteins and the VKOR is the rate limiting enzyme in this pathway. The product of VKOR1 gene encodes the enzyme that is responsible for reducing vitamin K 2,3-epoxide to the enzymatically activated form. Vitamin K epoxide reductase (VKOR) is the target of warfarin, the most widely prescribed anticoagulant for thromboembolic disorders and its discovery led to better understanding of blood coagulation and development of anti-coagulant drugs. Though VKOR was discovered in 1974, the enzyme has yet to be purified and characterized fully for its activity kinetics. Positional cloning approach has made possible to identify and map the VKOR gene to human chromosome 16p12-q21. The SiRNA experiment suggest that MGC11276 messenger RNA codes for 163 amino acid warfarin sensitive with at least one transmembrane domain VKOR1 protein (1). Coumarin derived drugs such as warfarin presents a long-term treatment and prevention of thromboembolic events by inhibiting VKOR multiprotein complex. Despite extensive efforts this complex has not yet fully identified. The VKOR complex recycle vitamin K 2,3-epoxide to vitamin K hydroquinone, a cofactor that is essential for the post translational gamma-carboxylation of several blood coagulation factors. The VKOR complex is implicated in at least 2 heritable human conditions, combined deficiency of vitamin-K-dependent clotting factors type 2 (VKCFD2; Online Mendelian Inheritance in Man (OMIM) 607473), and resistance to coumarin-type anticoagulant drugs (warfarin resistance, WR; OMIM 122700).
The vitamin K epoxide reductase complex subunit 1 (VKORC1), which encodes a small transmembrane protein of the endoplasmic reticulum. The VKORC1 contains missense mutations in both human disorders and in a warfarin-resistant rat strain. Overexpression of wild-type VKORC1, but not VKORC1 carrying the VKCFD2 mutation, leads to a marked increase in VKOR activity, which is sensitive to warfarin inhibition (3). Atleast two pseudo genes for VKORC1 have been identified on chromosome 1 and X along with several alternatively spliced transcripts encoding different isoforms. Recently a recombinant VKORC1 was purified from virally infected SF9 cells and VKOR activity was preserved in this highly purified fraction (4). The VKOR1 is associated with microsomal membrane preparation. VKORC1 is a 163 amino acid protein, the recombinant VKORC1 was purified as a 21kDa protein with tag protein from Sf9 cells.

General readings

Oldenburg J, Bevans CG, Muller CR, et al. Vitamin K epoxide reductase complex subunit 1 (VKORC1): the key protein of the vitamin K cycle. Antioxid. Redox Signal. 2006; 8:347-53.

19 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

VKORC1 (N-term) antibody

  Rabbit Polyclonal VKORC1 Antibody  
Western blot analysis of VKORC1 in A549 cell lysate with VKORC1 antibody at 1 ug/mL. Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

VKORC1 antibody

  Rabbit Polyclonal Anti-VKORC1 Antibody  
WB Suggested Anti-VKORC1 Antibody; Titration: 1.0 ug/ml; Positive Control: Fetal Brain Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

CRISPR

Catalog No. Species Donor Vector  

VKORC1 - human gene knockout kit via CRISPR

VKORC1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

VKORC1 - human gene knockout kit via CRISPR

VKORC1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

VKORC1 - human gene knockout kit via CRISPR

VKORC1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

VKORC1 - human gene knockout kit via CRISPR

VKORC1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Vkorc1 - mouse gene knockout kit via CRISPR

Vkorc1 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Vkorc1 - mouse gene knockout kit via CRISPR

Vkorc1 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Vkorc1 - mouse gene knockout kit via CRISPR

Vkorc1 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Vkorc1 - mouse gene knockout kit via CRISPR

Vkorc1 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

VKORC1 (transcript variant 2)

  Recombinant protein of human vitamin K epoxide reductase complex, subunit 1 (VKORC1), transcript variant 2  
VKORC1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

Lysates

Catalog No.    

VKORC1 overexpression lysate

VKORC1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

VKORC1 overexpression lysate

VKORC1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, VKORC1 (mGFP-tagged) - Human vitamin K epoxide reductase complex, subunit 1 (VKORC1), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, VKORC1 (mGFP-tagged) - Human vitamin K epoxide reductase complex, subunit 1 (VKORC1), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, VKORC1 (Myc-DDK tagged) - Human vitamin K epoxide reductase complex, subunit 1 (VKORC1), transcript variant 2 , 200 uL, >10^7 TU/mL

Lenti ORF particles, VKORC1 (Myc-DDK tagged) - Human vitamin K epoxide reductase complex, subunit 1 (VKORC1), transcript variant 2 , 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Vkorc1 (GFP-tagged) - Mouse vitamin K epoxide reductase complex, subunit 1 (Vkorc1), 200 uL, >10^7 TU/mL

Lenti ORF particles, Vkorc1 (GFP-tagged) - Mouse vitamin K epoxide reductase complex, subunit 1 (Vkorc1), 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, Vkorc1 (Myc-DDK-tagged) - Mouse vitamin K epoxide reductase complex, subunit 1 (Vkorc1), 200 uL, >10^7 TU/mL

Lenti ORF particles, Vkorc1 (Myc-DDK-tagged) - Mouse vitamin K epoxide reductase complex, subunit 1 (Vkorc1), 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

Rat Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Vkorc1 (GFP-tagged ORF) - Rat vitamin K epoxide reductase complex, subunit 1 (Vkorc1), 200 uL, >10^7 TU/mL

Lenti ORF particles, Vkorc1 (GFP-tagged ORF) - Rat vitamin K epoxide reductase complex, subunit 1 (Vkorc1), 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, Vkorc1 (Myc-DDK-tagged ORF) - Rat vitamin K epoxide reductase complex, subunit 1 (Vkorc1), 200 uL, >10^7 TU/mL

Lenti ORF particles, Vkorc1 (Myc-DDK-tagged ORF) - Rat vitamin K epoxide reductase complex, subunit 1 (Vkorc1), 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

19 Item(s)

per page
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