VPS28 antibody

Principal name

VPS28 antibody

Alternative names for VPS28 antibody

Vacuolar protein sorting-associated protein 28 homolog, ESCRT-I complex subunit VPS28

SwissProt ID

B5DEN9 (Rat), Q3T178 (Bovin), Q9D1C8 (Mouse), Q9UK41 (Human)

Gene ID

51160 (VPS28)

Available reactivities

Can (Canine), Hu (Human), Ms (Mouse), Por (Porcine), Rt (Rat), Xen (Xenopus), Ze (Zebrafish), Mky (Monkey), Bov (Bovine), Rb (Rabbit)

Available hosts

Goat, Rabbit, Mouse, Chicken

Available applications

Enzyme Immunoassay (E), Frozen Sections (C), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)

Background of VPS28 antibody

This gene encodes a protein involved in endosomal sorting of cell surface receptors via a multivesicular body/late endosome pathway. The encoded protein is one of the three subunits of the ESCRT-I complex (endosomal complexes required for transport) involved in the sorting of ubiquitinated proteins. The two other subunits of ESCRT-I are vesicular protein sorting 23, also known as tumor susceptibility gene 101 (TSG101), and vesicular protein sorting 37. Two alternative transcripts encoding different isoforms have been described. Additional alternative transcripts may exist but the proteins encoded by these transcripts have not been verified experimentally. Transcript Variant: This variant (2) includes an alternate segment, compared to variant 1, that causes a frameshift. The resulting protein (isoform 2) is longer and has a distinct C-terminus, compared to isoform 1.

General readings

Bishop N, Woodman P.
TSG101/mammalian VPS23 and mammalian VPS28 interact directly and are recruited to VPS4-induced endosomes.
J Biol Chem. 2001 Apr 13;276(15):11735-42.
PMID: 11134028

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  2. Highly Validated (1)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

VPS28 antibody

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-VPS28 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505691) Mouse IgG1 OTI1A8 Purified Can, Hu, Mky, Ms, Rt ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

VPS28 (C-term) antibody

TA302924 staining (1µg/ml) of Human Testis lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Por, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

VPS28 antibody

WB Suggested Anti-VPS28 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Human Liver Rabbit IgG Purified Bov, Can, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

VPS28 antibody

VPS28 Mouse Hu WB
0.5 mg / €1,400.00
  Abnova Taiwan Corp.

VPS28 antibody

VPS28 polyclonal antibody ( Cat # PAB6584 ) ( 10 µg/ml ) staining of paraffin embedded human testis. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. Goat Hu E, P, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

VPS28 antibody

mouse brain extract; WB Suggested Anti-VPS28 Antibody . . Positive Control: Lane 1: 20ug mouse brain extract Lane 2: 20ug mouse brain extract. Primary Antibody Dilution : 1:500. Secondary Antibody : Anti rabbit-HRP . Secondry Antibody Dilution : 1:5,000. Submitted by: Scott Wilson, University of Alabama at Birmingham; VPS28 antibody - N-terminal region (ARP56850_P050) in mouse brain extract cells using Western Blot Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

VPS28 antibody

NB100-1399 (10µg/ml) staining of paraffin embedded Human Testis. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. Goat IgG Aff - Purified Hu E, P, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

VPS28 antibody

VPS28 Rabbit IgG Purified Hu, Ms, Rt, Xen, Ze C, P, WB
0.5 mg / €400.00
  Novus Biologicals Inc.

VPS28 antibody

Immunohistochemistry-Paraffin: VPS28 Antibody [NBP1-85973] - Immunohistochemical staining of human rectum shows strong granular cytoplasmic positivity in glandular cells. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

VPS28 (transcript variant 1)

VPS28 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

VPS28 (transcript variant 2)

VPS28 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

VPS28 (1-221)

VPS28 Human Purified > 95 % by SDS - PAGE E. coli
0.5 mg / €540.00
  Acris Antibodies GmbH

VPS28 (1-221)

VPS28 Human Purified > 95 % by SDS - PAGE E. coli
0.1 mg / €230.00
  Acris Antibodies GmbH

VPS28

VPS28 Human
0.1 mg / €330.00
  Abnova Taiwan Corp.

VPS28

Western Blot: VPS28 Protein [NBP1-30248] - 15% SDS-PAGE (3ug)
0.1 mg / €310.00
  Novus Biologicals Inc.

Lysates

Catalog No.    

VPS28 overexpression lysate

VPS28 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

16 Item(s)

per page
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