VSIG1 antibody

Principal name

VSIG1 antibody

Alternative names for VSIG1 antibody

Cell surface A33 antigen, Glycoprotein A34, GPA34, V-set and immunoglobulin domain-containing protein 1

SwissProt ID

Q29RR6 (Bovin), Q4KLY3 (Rat), Q86XK7 (Human), Q91664 (Xenla), Q9D2J4 (Mouse), Q9PWR4 (Chick)

Gene ID

340547 (VSIG1), 414795

Available reactivities

Hu (Human), Chk (Chicken), Eq (Equine), Por (Porcine)

Available hosts

Mouse, Rabbit

Available applications

ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Functional assay (FN), Immunoprecipitation (IP), Flow Cytometry (F), Enzyme Immunoassay (E), Paraffin Sections (P)

Background of VSIG1 antibody

ChT1, a member of the Ig superfamily with one V-like and one C2-like domain, is a T cell antigen that is expressed on the surface of embryonic thymocytes (day 10). In young chickens, about 90% of the thymocytes as well as a subpopulation of peripheral lymphocytes (which represent recent thymic emigrants) are ChT1 positive.1-4 Expression in the periphery declines with age and, in correlation with partial thymectomy, indicates that ChT1 can be used as an accurate marker for studying thymic function. (3) CT1 can block T cell differentiation in vitro, in thymic organ cultures and in thymocyte precursors cultured on stromal cell monolayers. (4) CT1 also recognizes quail cortical thymocytes. (1)

General readings

1. Chen, C.L., H.T.C. Chanh, and M.D. Cooper. 1984. Chicken thymocyte-specific antigen identified by monoclonal antibodies: ontogeny,
tissue distribution and biochemical characterization. Eur. J. Immunol. 14:385.
2. Houssaint, E., E. Dietz, and F.V. Jotereau. 1985. Tissue distribution and ontogenic appearance of a chicken T lymphocyte differentiation marker. Eur. J. Immunol. 15:305-308.
3. Kong, F-K., C.L.H. Chen, and M.D. Cooper. 1998. Thymic function can be accurately monitored by the level of recent T cell emigrants in the circulation. Immunity: 8:97-104.
4. Katevuo, K., B.A., Imhof, R. Boyd., A. Chidgey, A. Bean, D. Dunon, T.W.F. Gobel, and O. Vainio. 1999. ChT1, an Ig superfamily molecule required for T cell differentiation. J. Immunol. 162:5685-5694.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

VSIG1 antibody

WB Suggested Anti-VSIG1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: MCF7 cell lysate Rabbit IgG Purified Eq, Hu, Por WB
50 µg / €325.00
  OriGene Technologies, Inc.

VSIG1 antibody

VSIG1 Mouse IgG1 CT1 Biotin Chk F
0.5 mg / €410.00
  Acris Antibodies GmbH

VSIG1 antibody

Immunofluorescent Staining: Chicken thymocytes were double stained with Mouse anti-Chicken CD3-PE and Mouse anti-Chicken ChT1-FITC. Analysis was performed on a FACScan(TM) flow cytometer (BDB, San Jose, CA). Amount Used: < / = 1 µg/10e6 cells. Mouse IgG1 CT1 FITC Chk F
0.5 mg / €450.00
  Acris Antibodies GmbH

VSIG1 antibody

VSIG1 Mouse IgG1 CT1 Purified Chk F, FN, IP
0.5 mg / €340.00
  Acris Antibodies GmbH

VSIG1 antibody

VSIG1 Mouse IgG1 CT1 PE Chk F
0.1 mg / €320.00
  Acris Antibodies GmbH

VSIG1 (C-term) antibody

VSIG1 Antibody (C-term) (AP54520PU-N)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of VSIG1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

VSIG1 antibody

Western Blot analysis of VSIG1 expression in transfected 293T cell line (H00340547-T01) by VSIG1 MaxPab polyclonal antibody.

Lane 1: VSIG1 transfected lysate(42.57 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

VSIG1 antibody

Chicken thymocytes were double stained with mouse anti-chicken CD3-R-phycoerythrinand VSIG1 monoclonal antibody, clone CT1 ( Cat # MAB3280 )-FITC. Analysis was performed on a FACScanTM flow cytometer ( BDB, San Jose, CA ) using a light scatter gate to identify small lymphocytes. Mouse IgG1 CT1 Chk F, IP
0.5 mg / €390.00
  Abnova Taiwan Corp.

VSIG1 antibody

Human MCF-7; WB Suggested Anti-VSIG1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: MCF7 cell lysate; VSIG1 antibody - N-terminal region (ARP55832_P050) in Human MCF-7 cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

VSIG1 antibody

Flow cytometry / FACS analysis: ChT1 antigen Antibody (CT1) [NBP1-28355] - Chicken thymocytes were double stained with mouse anti-chicken CD3-PE and mouse antichicken ChT1-FITC. Analysis was performed on a FACScanTM flow cytometer (BDB, San Jose, CA) using a light scatter gate to identify small lymphocytes. Mouse IgG1 CT1 Purified Chk F, IP
0.5 mg / €470.00
  Novus Biologicals Inc.

VSIG1 antibody

Flow cytometry: ChT1 Antibody (CT1) [NBP1-28356] - #8804; 1 ug/10^6 Chicken thymocytes were double stained with mouse anti-chicken CD3-PE and mouse anti-chicken  ChT1-FITC. Analysis was performed on a flow cytometer using a light scatter gate to identify small lymphocytes. Mouse IgG1 CT1 FITC Chk F, IP
0.25 mg / €600.00
  Novus Biologicals Inc.

VSIG1 antibody

Flow cytometry / FACS analysis: ChT1 antigen Antibody (CT1) [Biotin] [NBP1-28357] - Chicken thymocytes were double stained with mouse anti-chicken CD3-PE and mouse antichicken ChT1-FITC. Analysis was performed on a FACScanTM flow cytometer (BDB, San Jose, CA) using a light scatter gate to identify small lymphocytes. Mouse IgG1 CT1 FITC Chk F, IP
0.25 mg / €600.00
  Novus Biologicals Inc.

VSIG1 antibody

Flow cytometry / FACS analysis: ChT1 antigen Antibody (CT1) [R-Phycoerythrin] [NBP1-28358] - Chicken thymocytes were double stained with mouse anti-chicken CD3-PE and mouse antichicken ChT1-FITC. Analysis was performed on a FACScanTM flow cytometer (BDB, San Jose, CA) using a light scatter gate to identify small lymphocytes. Mouse IgG1 CT1 PE Chk F, IP
0.1 mg / €380.00
  Novus Biologicals Inc.

VSIG1 antibody

Immunohistochemistry-Paraffin: VSIG1 Antibody [NBP1-81072] - Immunohistochemical staining of human testis shows strong cytoplasmic and membranous positivity in cells in seminiferus ducts. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

VSIG1 antibody

Immunohistochemistry-Paraffin: VSIG1 Antibody [NBP1-81073] - Immunohistochemical staining of human stomach shows strong membranous and cytoplasmic positivity in glandular cells. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

VSIG1 antibody

Immunohistochemistry-Paraffin: VSIG1 Antibody [NBP1-81074] - Immunohistochemical staining of human stomach shows strong membranous and cytoplasmic positivity in glandular cells. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

VSIG1

VSIG1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

VSIG1

VSIG1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

VSIG1 293T Cell Transient Overexpression Lysate(Denatured)

VSIG1 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

VSIG1 overexpression lysate

VSIG1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

20 Item(s)

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