WAS / IMD2 antibody

Principal name

WAS / IMD2 antibody

Alternative names for WAS / IMD2 antibody

Wiskott-Aldrich syndrome protein, WASp

SwissProt ID

P42768 (Human), P70315 (Mouse)

Gene ID

7454 (WAS)

Available reactivities

Hu (Human), Ms (Mouse), Can (Canine), Rt (Rat)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F)

Background of WAS / IMD2 antibody

Wiskott-Aldrich syndrome proteins (WASPs) mediate actin dymics by activating the Arp2/3 actin nucleation complex in response to activated Rho family GTPases. In mammals, five WASP family members have been described. Hematopoietic WASP and ubiquitously expressed N-WASP are autoinhibited in unstimulated cells. Upon stimulation they are activated by cdc42, which relieves the autoinhibition in conjunction with phosphatidyl inositol 4,5-bisphosphate. Three WAVE (Wasf, SCAR) family proteins are similar in sequence to WASP and N-WASP but lack the WASP/N-WASP autoinhibition domains and are indirectly activated by Rac . Both WASP and WAVE functions appear to be essential, as knockout of either N-WASP or Scar-2 in mice results in cardiac and neurol defects and embryonic lethality . Loss of WASP results in immune system defects and fewer immune cells . WAVE-2 (WASF2) is widely distributed, while WAVE-1 and WAVE-3 are strongly expressed in brain . WAVE-3 may act as a tumor suppressor in neuroblastoma, a childhood disease of the sympathetic nervous system . Increased expression of WAVE-3 is seen in breast cancer, and studies in breast adenocarcinoma cells indicate that WAVE-3 regulates breast cancer progression, invasion and metastasis through the p38 mitogen-activated protein kise (MAPK) pathway .

General readings

Wu X et al. Focal adhesion kinase regulation of N-WASP subcellular localization and function. J Biol Chem 279:9565-76 (2004). PubMed PMID: 14676198
Torres E & Rosen MK Contingent phosphorylation/dephosphorylation provides a mechanism of molecular memory in WASP. Mol Cell 11:1215-27 (2003). PubMed PMID: 12769846

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

WAS / IMD2 antibody

TA303004 staining (0.03µg/ml) of U937 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

WAS / IMD2 antibody

Predicted band size: 53 kDa . Positive control: Hela, Jurkat and Raji cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lane1,2,3: Hela, Jurkat and Raji cells lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

WAS / IMD2 antibody

Western blot analysis of extracts of variouscell lines, using WAS antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

WAS / IMD2 antibody

Western blot analysis of extract from Hela, JK and Raji cells using WASP Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

WAS / IMD2 antibody

Immunohistochemistry (IHC) staining of Paraffin-Embedded Human breast carcinoma tissue using WASP antibody (Cat.-No.: AP06593PU-N) . Rabbit Aff - Purified Hu, Ms P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

WAS / IMD2 (Center) antibody

WAS Antibody (Center) (AP54531PU-N) IHC analysis in formalin fixed and paraffin embedded tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the WAS Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu, Ms F, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

WAS / IMD2 antibody

WAS / IMD2 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

WAS / IMD2 antibody

WAS / IMD2 Mouse IgG2b 3H5 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

WAS / IMD2 antibody

WAS polyclonal antibody ( Cat # PAB6757 ) staining ( 0.03 µg/ml ) of U-937 lysate ( RIPA buffer, 30 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

WAS / IMD2 pTyr290 antibody

Immunohistochemistry analysis of paraffin-embedded human brain, using WASP (Phospho-Tyr290) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P, WB
50 µg / €205.00
  Assay Biotech

WAS / IMD2 pTyr290 antibody

Immunohistochemistry analysis of paraffin-embedded human brain, using WASP (Phospho-Tyr290) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P, WB
0.1 mg / €310.00
  Assay Biotech

WAS / IMD2 antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using WASP (Ab-290) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P, WB
50 µg / €205.00
  Assay Biotech

WAS / IMD2 antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using WASP (Ab-290) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P, WB
0.1 mg / €310.00
  Assay Biotech

WAS / IMD2 pTyr290 antibody

Western blot analysis of WASP (pY290) expression in Jurkat (A); TF1 (B) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt F, ICC/IF, P, WB
30 µl / €210.00
  Cohesion Biosciences Ltd.

WAS / IMD2 pTyr290 antibody

Western blot analysis of WASP (pY290) expression in Jurkat (A); TF1 (B) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt F, ICC/IF, P, WB
0.1 ml / €330.00
  Cohesion Biosciences Ltd.

WAS / IMD2 pTyr290 antibody

Western blot analysis of WASP (pY290) expression in Jurkat (A); TF1 (B) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt F, ICC/IF, P, WB
0.2 ml / €530.00
  Cohesion Biosciences Ltd.

WAS / IMD2 antibody

Western Blot: WASP Phosphospecific Antibody [NB100-2307] - Detection of Human Phospho-WASP-S483/S484 by Western Blot.  Samples: Whole cell lysate (50 mcg/lane) from U937 and Jurkat cells. Lysates were either untreated, mock treated with phosphatase or treated with phosphatase.  Antibody:  Affinity purified rabbit anti-phospho WASP-S483/S484 NB100-2307 used at 1 mcg/ml.  Detection: Chemiluminescence with an exposure time of 30 seconds. Rabbit Aff - Purified Hu WB
0.1 mg / €490.00
  Novus Biologicals Inc.

WAS / IMD2 antibody

NB 300-996 staining (0.03ug/ml) of U937 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat IgG Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

WAS / IMD2 antibody

Western Blot: N-WASP [Tyr256] Antibody [NB600-1169] - Western blot analysis of control and pervanadate-treated A431 cells (20 µg/lane). Blots were probed with either rabbit polyclonal anti-N-WASP (Ser484/Ser485) or anti-N-WASP (Tyr256). Rabbit IgG Aff - Purified Hu E, WB
50 µl / €400.00
  Novus Biologicals Inc.

WAS / IMD2 antibody

Western Blot: WASp Antibody (EPR2540Y) [NBP1-40425] - Western blot analysis on (A) TF-1 (B) U937 cell lysate and (C) Jurkat cell lysate using anti-WASP, dilution 1:2000. Rabbit IgG EPR2540Y Hu, Ms, Rt F, ICC/IF, IP, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

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