WAS / IMD2 antibody

Principal name

WAS / IMD2 antibody

Alternative names for WAS / IMD2 antibody

Wiskott-Aldrich syndrome protein, WASp

SwissProt ID

P42768 (Human), P70315 (Mouse)

Gene ID

7454 (WAS), 22376 (Was)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of WAS / IMD2 antibody

Wiskott-Aldrich syndrome proteins (WASPs) mediate actin dymics by activating the Arp2/3 actin nucleation complex in response to activated Rho family GTPases. In mammals, five WASP family members have been described. Hematopoietic WASP and ubiquitously expressed N-WASP are autoinhibited in unstimulated cells. Upon stimulation they are activated by cdc42, which relieves the autoinhibition in conjunction with phosphatidyl inositol 4,5-bisphosphate. Three WAVE (Wasf, SCAR) family proteins are similar in sequence to WASP and N-WASP but lack the WASP/N-WASP autoinhibition domains and are indirectly activated by Rac . Both WASP and WAVE functions appear to be essential, as knockout of either N-WASP or Scar-2 in mice results in cardiac and neurol defects and embryonic lethality . Loss of WASP results in immune system defects and fewer immune cells . WAVE-2 (WASF2) is widely distributed, while WAVE-1 and WAVE-3 are strongly expressed in brain . WAVE-3 may act as a tumor suppressor in neuroblastoma, a childhood disease of the sympathetic nervous system . Increased expression of WAVE-3 is seen in breast cancer, and studies in breast adenocarcinoma cells indicate that WAVE-3 regulates breast cancer progression, invasion and metastasis through the p38 mitogen-activated protein kise (MAPK) pathway .

General readings

Yamaguchi H, Lorenz M, Kempiak S, Sarmiento C, Coniglio S, Symons M, Segall J, Eddy R, Miki H, Takenawa T, Condeelis J. Molecular mechanisms of invadopodium formation: the role of the N-WASP-Arp2/3 complex pathway and cofilin. J Cell Biol. 2005 Jan 31;168(3):441-52.

3 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

WAS / IMD2 antibody

  Anti-WAS Rabbit Polyclonal Antibody  
Predicted band size: 53 kDa . Positive control: Hela, Jurkat and Raji cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lane1,2,3: Hela, Jurkat and Raji cells lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

WAS / IMD2 antibody

  WAS Rabbit Polyclonal Antibody  
Western blot analysis of extracts of variouscell lines, using WAS antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €335.00
  OriGene Technologies, Inc.

WAS / IMD2 antibody

  Rabbit Polyclonal Anti-WASP Antibody  
Western blot analysis of extract from Hela, JK and Raji cells using WASP Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €335.00
  OriGene Technologies, Inc.

3 Item(s)

per page
  • LinkedIn