WDHD1 antibody

Principal name

WDHD1 antibody

Alternative names for WDHD1 antibody

AND1, AND-1, Acidic nucleoplasmic DNA-binding protein 1, WD repeat and HMG-box DNA-binding protein 1

SwissProt ID

O13046 (Xenla), O75717 (Human), P59328 (Mouse)

Gene ID

11169 (WDHD1)

Available reactivities

Bov (Bovine), Can (Canine), Chk (Chicken), Hu (Human), Ms (Mouse), Rt (Rat), African clawed frog, Xen (Xenopus), Eq (Equine), Por (Porcine), Rb (Rabbit), Ye (Yeast)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Enzyme Immunoassay (E), Paraffin Sections (P)

Background of WDHD1 antibody

AND-1 (WDHD1) also known as WD repeat and HMG box DNA binding protein 1, acidic nucleoplasmic DNA-binding protein 1. AND-1 (WDHD1) is a nuclear and nucleolar protein that contains multiple WD40 repeats, and one HMG region. AND-1 (WDHD1) has a predicted molecular weight of 126 kD and can form homodimers. WDHD1 is expressed in the brain, epidermis, liver, and stomach. During mitosis, AND-1 (WDHD1) is transiently expressed in nucleoplasm. AND-1 (WDHD1) can bind to DNA with high affinity and may be involved in protein-protein interactions. The role of AND-1 (WDHD1) is thought to be chromatin assembly, transcription and DNA replication. AND-1 (WDHD1) has been reported to be phosphorylated on S1041. 
Structure: Contains multiple WD40 repeats, one HMG region. Predicted molecular weight 126 kD. Forms homodimers.
Distribution: Found in the nucleus and nucleolus. Expressed in brain, epidermis, liver, and stomach. During mitosis transiently expressed in nucleoplasm.
Function: Binds DNA with high affinity; may be involved in protein-protein interactions. Thought to function in chromatin assembly, transcription and DNA replication.
Modification: Phosphorylation (S1041).

General readings

Zhu,W., (2007) Genes Dev. 21 (18), 2288-2299

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

WDHD1 antibody

Western blot analysis of extracts from HepG2 cells and Jurkat cells, using WDHD1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

WDHD1 (C-term) antibody

Jurkat nuclear cell extracts (lane 1) and NIH3T3 nuclear extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-AND-1 antibody (clone 20G10). Proteins were visualized using a goat anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection. Mouse IgG2a 20G10 Aff - Purified Hu, Ms P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

WDHD1 antibody

WB Suggested Anti-WDHD1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:12500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

WDHD1 antibody

WB Suggested Anti-WDHD1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Hela cell lysate Rabbit IgG Purified Bov, Can, Eq, Hu, Por, Rb, Ye WB
50 µg / €325.00
  OriGene Technologies, Inc.

WDHD1 (C-term) antibody

Jurkat nuclear cell extracts (lane 1) and NIH3T3 nuclear extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-AND-1 antibody (clone 20G10). Proteins were visualized using a goat anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection. Mouse IgG2a 20G10 Aff - Purified Hu, Ms P, WB
25 µg / €230.00
  Acris Antibodies GmbH

WDHD1 antibody

WDHD1 Rabbit Serum Hu C, E, WB
0.2 ml / €400.00
  Acris Antibodies GmbH

WDHD1 antibody

WDHD1 Mouse IgG1 23-5-14 Supernatant Xen C, ICC/IF, IP, WB
0.25 ml / €360.00
  Acris Antibodies GmbH

WDHD1 antibody

WDHD1 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

WDHD1 antibody

WDHD1 Mouse IgG1 2F10 Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

WDHD1 antibody

Western blot analysis of extracts from Jurkat/HepG2 cells, using WDHD1 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, WB
50 µg / €205.00
  Assay Biotech

WDHD1 antibody

Western blot analysis of extracts from Jurkat/HepG2 cells, using WDHD1 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, WB
0.1 mg / €310.00
  Assay Biotech

WDHD1 antibody

Human HepG2; WB Suggested Anti-WDHD1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: HepG2 cell lysate; WDHD1 antibody - middle region (ARP32844_P050) in Human HepG2 cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

WDHD1 antibody

Human HeLa; WB Suggested Anti-WDHD1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Hela cell lysate; WDHD1 antibody - C-terminal region (ARP38902_P050) in Human HeLa cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu WB
50 µg / €350.00
  AVIVA Systems Biology

WDHD1 antibody

Immunohistochemical analysis of WDHD1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

WDHD1 antibody

Immunohistochemical analysis of WDHD1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

WDHD1 antibody

Immunohistochemical analysis of WDHD1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

WDHD1 antibody

WDHD1 Rabbit IgG Aff - Purified Hu E, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

WDHD1 antibody

WDHD1 Rabbit IgG Aff - Purified Hu E, WB
50 µg / €290.00
  Novus Biologicals Inc.

WDHD1 antibody

Immunohistochemistry-Paraffin: WDHD1 Antibody [NBP1-89091] -  Staining of human stomach shows strong nuclear positivity in glandular cells. Rabbit IgG Aff - Purified Hu, Rt ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

WDHD1 (transcript variant 1)

WDHD1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

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