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WDR46 / BING4 antibody

Principal name

WDR46 / BING4 antibody

Alternative names for WDR46 / BING4 antibody

C6orf11, FP221, WD repeat-containing protein 46, WD repeat-containing protein BING4

SwissProt ID

O15213 (Human), Q9Z0H1 (Mouse)

Gene ID

9277 (WDR46)

Ncbi ID

9606, NP_001157739.1, NP_005443

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of WDR46 / BING4 antibody

WDR46 was originally identified as BING-4, a protein encoded in a gene-rich region of the major histocompatibility complex (MHC). WDR46 has been found to be overexpressed in a subset of melanoma cell lines and is a cancer antigen recognized by lymphocytes. WDR46 bears six WD repeats. The WD repeat is defined by four or more repeating units of a conserved core of approximately 40 amino acids ending with tryptophan-aspartic acid (WD). WD repeats may serve as sites of protein-protein interaction for adaptor proteins and facilitate multiprotein complex formation. WD proteins are involved in a variety of cellular processes. The function of WDR46 has not been characterized.

Western Blot: WDR46 Antibody [NB100-77318] - Detection of Human WDR46 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-WDR46 antibody NB100-77318 used for IP at 3 mcg/mg lysate. WDR46 was also immunoprecipitated by rabbit anti-WDR46 antibody NB100-77301, which recognizes a downstream epitope. Detection: Chemiluminescence with an exposure time of 30 seconds.

NB100-77318

Western Blot: WDR46 Lysate [NBL1-17812] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for WDR46

NBL1-17812

HeLa cells were subjected to SDS PAGE followed by western blot with 15110-1-AP(WDR46 antibody) at dilution of 1:400

15110-1-AP

Jurkat cells were subjected to SDS PAGE followed by western blot with 15110-1-AP(WDR46 antibody) at dilution of 1:400

15110-1-AP

Immunohistochemistry-Paraffin: WDR46 Antibody [NBP1-94128] Immunohistochemical staining of human thyroid gland shows strong cytoplasmic positivity in glandular cells.

NBP1-94128

Primary Antibodies

Catalog No.	Host	Iso.	Pres.	React.	Applications
NB100-77318	WDR46 / BING4 antibody
	Rabbit	IgG	Aff - Purified	Hu	IP, WB	0.1 mg / €440.00
						Novus Biologicals Inc.
NBP1-94128	WDR46 / BING4 antibody
	Rabbit	IgG	Aff - Purified	Hu	P	0.1 ml / €500.00
						Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No.	Species	Pres.	Purity	Source
TP301770	WDR46 / BING4
	Human		> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €680.00
					OriGene Technologies, Inc.
H00009277-P01	WDR46 / BING4
	Human	Purified			2 µg / €300.00
					Abnova Taiwan Corp.

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