WDR5 / BIG3 antibody

Principal name

WDR5 / BIG3 antibody

Alternative names for WDR5 / BIG3 antibody

BIG-3, WD repeat-containing protein 5, BMP2-induced 3-kb gene protein

SwissProt ID

P61964 (Human), P61965 (Mouse), Q2KIG2 (Bovin), Q498M4 (Rat)

Gene ID

11091 (WDR5), 140858 (Wdr5)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Ye (Yeast), Ze (Zebrafish)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P)

Background of WDR5 / BIG3 antibody

The WD40-repeat proteins are a large family of proteins found in all eukaryotes and implicated in very diverse functions such as signal transduction, vesicular trafficking, cytoskeletal assembly, cell cycle control, apoptosis and transcription regulation. A general function arttributed to WD40 contaiing proteins is to provide scaffold for protein-protein interaction and initiate/coordinate down-stream events such as ubiquitination methylation and acetylation. One of the WD40 repeat protein WDR5 directly interacts with K4 methylated H3 (1). The WDR5 interaction with K4 methylated H3 presents a novel dimethyl lysine recognition interaction which differs form chromodomain and or tudor domain interactions with acetylated histones requiring an aromatic cage to recognize methylated lysines. The interaction of WDR5 with H3K4me3 is via nonconventional hydrogen bonds involving the carboxylgroup of Glu322 (2). Other reports suggest that dimethylation of H3K4 is modestly recognize by WDR5 (3) and other report suggest that methylation state of K4 has no effect on H3 binding to WDR5 (4).
WDR5 is a highly conserved protein that shares over 90% sequence identity among all vertebrates, and 68 and 34% sequence identity with Caenorhabditis elegans and Saccharomyces cerevisiae WDR5 homologs, respectively (2). WDR5 contains seven WD40 repeats, forming a seven-blade β-propeller structure preceded by 30 amino acid unstructured loop. WDR5 is developmentally expressed in osteoblasts and is invoiced in the accelerated differentiation, suppression of WDR5 interferes with canonical Wnt signaling pathways at multiple levels and optimal levels are required for osteoblast development (5).
WDR5 is a 334 (40kDa) amino acid nuclear protein. WD40 domain, found in a number of eukaryotic proteins which contain a GH dipeptide of 11-24 amino acid in residues and is involved in a wide variety of functions such as acting like an adaptor/regulatory modules in signal transduction,pre-mRNA processing and cytoskeleton assembly. The WD40 domain of WDR5 represents a histone methyl-lysine recognition domains that is important for recruiting H3K4 methyltransferases to K4-dimethylated histone H3 tail as well as for global and gene-specific K4 trimethylation (1).

General readings

Smith TF, Gaitatzes C, Saxena K, et al. The WD repeat: a common architecture for diverse functions. Trends Biochem. Sci. 1999; 24:181-5.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

WDR5 / BIG3 antibody

Immunofluorescence of monoclonal antibody to WDR5 on HeLa cell. [antibody concentration 10 ug/ml] Mouse IgG2b 2C2 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

WDR5 / BIG3 antibody

Western blot analysis of WDR5 in 293 cell lysate with WDR5 antibody at 1 µg/ml in (A) the absence and (B) the presence of blocking peptide. Rabbit Purified Hu E, ICC/IF, WB
0.1 mg / €379.00
  OriGene Technologies, Inc.

WDR5 / BIG3 (N-term) antibody

Western blot analysis of WDR5 in 293 cell lysate with WDR5 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide. Rabbit IgG Aff - Purified Hu E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

WDR5 / BIG3 antibody

Western blot analysis of extracts of various cell lines, using WDR5 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

WDR5 / BIG3 antibody

WB Suggested Anti-WDR5 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: 721_B cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rt, Ye, Ze IP, P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

WDR5 / BIG3 antibody

WB was performed using the WDR5 ab at 1:1000 in TBS-Tween containing with 5% skimmed milk on the purified recombinant protein (lanes 1-3), ectopically expressed FLAG-tagged WDR5 (WDR5-F) which was first IP'd with anti-FLAG (lane 4) and nuclear extracts from HeLa (lane 6) and HEK293 (lane 7). Lane 5 (MOCK) represents a negative control. B: WB was performed using nuclear extracts from HeLa (HeLa NE, 20 ug) and the WDR5 ab at 1:1,000 in TBS-Tween with 5% skimmed milk. Rabbit IgG Purified Hu
0.1 ml / €325.00
  OriGene Technologies, Inc.

WDR5 / BIG3 antibody

WB was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the antibody against mouse Wdr5, diluted 1:1,000 in BSA/PBS-Tween. The molecular weight marker (M, in kDa) is shown on the left; the location of the protein of interest (36 kDa) is indicated on the right. Rabbit IgG Purified Ms E
0.1 ml / €325.00
  OriGene Technologies, Inc.

WDR5 / BIG3 antibody

WB using the antibody against WDR5 diluted of 1:1000 in TBS-Tween containing 5% skimmed milk (lane 2). The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right. Rabbit IgG Purified Hu
50 µg / €325.00
  OriGene Technologies, Inc.

WDR5 / BIG3 antibody

Western blot analysis using WDR5 mouse mAb AM06480SU-N against Hela (1) cell lysate. Mouse IgG2b 7B11 Ascites Hu E, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

WDR5 / BIG3 control peptide

WDR5 / BIG3
0.1 mg / €250.00
  Acris Antibodies GmbH

WDR5 / BIG3 (157-174) antibody

WDR5 / BIG3 Rabbit IgG Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

WDR5 / BIG3 (N-term) antibody

WDR5 Antibody (N-term) (AP54548PU-N) western blot analysis in A2058 cell line lysates (35 µg/lane). This demonstrates the WDR5 antibody detected the WDR5 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

WDR5 / BIG3 (N-term) Control Peptide

Western blot analysis of WDR5 in 293 cell lysate with WDR5 antibody AP55430PU-N at 1 µg/ml in (A) the absence and (B) the presence of blocking peptide.
50 µg / €160.00
  Acris Antibodies GmbH

WDR5 / BIG3 antibody

WDR5 polyclonal antibody (A01), Lot # FAK0060414QCS1 Western Blot analysis of WDR5 expression in A-549 ( Cat # L025V1 ). Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

WDR5 / BIG3 antibody

WDR5 monoclonal antibody (M01), clone 2C2. Western Blot analysis of WDR5 expression in Hs 181.Tes. Mouse IgG2b 2C2 Ascites Hu E, WB
0.2 ml / €370.00
  Abnova Taiwan Corp.

WDR5 / BIG3 antibody

WDR5 / BIG3 Mouse IgG2a 1B1 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

WDR5 / BIG3 antibody

Western blot was performed on whole cell lysates from mouse fibroblasts ( Lane 1, NIH/3T3 ) and embryonic stem cells ( Lane 2, E14Tg2a ) with Wdr5 polyclonal antibody ( Cat # PAB14068 ), diluted 1 : 1,000 in BSA/PBS-Tween. Rabbit Ms E, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

WDR5 / BIG3 (C-term) antibody

Mouse Pancreas; WB Suggested Anti-Wdr5 Antibody. . Titration: 1.0 ug/ml. . Positive Control: Mouse Pancreas; Wdr5 antibody - C-terminal region (ARP41307_P050) in Mouse Pancreas cells using Western Blot Rabbit Aff - Purified Hu, Ms WB
50 µg / €350.00
  AVIVA Systems Biology

WDR5 / BIG3 antibody

Western Blot: WDR5 Antibody (7B11) [NBP1-51629] - Western blot analysis using WDR5 mouse mAb against Hela (1) cell lysate. Mouse IgG2b 7B11 Purified Hu E, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

WDR5 / BIG3 (transcript variant 2)

WDR5 / BIG3 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

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