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WDR5 / BIG3 antibody

Principal name

WDR5 / BIG3 antibody

Alternative names for WDR5 / BIG3 antibody

BIG-3, WD repeat-containing protein 5, BMP2-induced 3-kb gene protein

SwissProt ID

P61964 (Human), P61965 (Mouse), Q2KIG2 (Bovin), Q498M4 (Rat)

Gene ID

11091 (WDR5), 140858 (Wdr5), 362093

Ncbi ID

9606, 10090, NP_060058, NM_080848, NP_060058.1, NM_017588, NM_052821, NP_438172, NP_543124.1, NM_001039034

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Ye (Yeast), Ze (Zebrafish)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Paraffin Sections (P)

Background of WDR5 / BIG3 antibody

The WD40-repeat proteins are a large family of proteins found in all eukaryotes and implicated in very diverse functions such as signal transduction, vesicular trafficking, cytoskeletal assembly, cell cycle control, apoptosis and transcription regulation. A general function arttributed to WD40 contaiing proteins is to provide scaffold for protein-protein interaction and initiate/coordinate down-stream events such as ubiquitination methylation and acetylation. One of the WD40 repeat protein WDR5 directly interacts with K4 methylated H3 (1). The WDR5 interaction with K4 methylated H3 presents a novel dimethyl lysine recognition interaction which differs form chromodomain and or tudor domain interactions with acetylated histones requiring an aromatic cage to recognize methylated lysines. The interaction of WDR5 with H3K4me3 is via nonconventional hydrogen bonds involving the carboxylgroup of Glu322 (2). Other reports suggest that dimethylation of H3K4 is modestly recognize by WDR5 (3) and other report suggest that methylation state of K4 has no effect on H3 binding to WDR5 (4).
WDR5 is a highly conserved protein that shares over 90% sequence identity among all vertebrates, and 68 and 34% sequence identity with Caenorhabditis elegans and Saccharomyces cerevisiae WDR5 homologs, respectively (2). WDR5 contains seven WD40 repeats, forming a seven-blade β-propeller structure preceded by 30 amino acid unstructured loop. WDR5 is developmentally expressed in osteoblasts and is invoiced in the accelerated differentiation, suppression of WDR5 interferes with canonical Wnt signaling pathways at multiple levels and optimal levels are required for osteoblast development (5).
WDR5 is a 334 (40kDa) amino acid nuclear protein. WD40 domain, found in a number of eukaryotic proteins which contain a GH dipeptide of 11-24 amino acid in residues and is involved in a wide variety of functions such as acting like an adaptor/regulatory modules in signal transduction,pre-mRNA processing and cytoskeleton assembly. The WD40 domain of WDR5 represents a histone methyl-lysine recognition domains that is important for recruiting H3K4 methyltransferases to K4-dimethylated histone H3 tail as well as for global and gene-specific K4 trimethylation (1).

General readings

Smith TF, Gaitatzes C, Saxena K, et al. The WD repeat: a common architecture for diverse functions. Trends Biochem. Sci. 1999; 24:181-5.

Immunofluorescence of monoclonal antibody to WDR5 on HeLa cell. [antibody concentration 10 ug/ml]

H00011091-M01

WDR5 monoclonal antibody (M01), clone 2C2. Western Blot analysis of WDR5 expression in Hs 181.Tes.

H00011091-M01

WDR5 monoclonal antibody (M01), clone 2C2. Western Blot analysis of WDR5 expression in A-549 ( Cat # L025V1 ).

H00011091-M01

Detection limit for recombinant GST tagged WDR5 is approximately 0.3ng/ml as a capture antibody.

H00011091-M01

WDR5 polyclonal antibody (A01), Lot # FAK0060414QCS1 Western Blot analysis of WDR5 expression in A-549 ( Cat # L025V1 ).

H00011091-A01

WDR5 monoclonal antibody (M01), clone 2C2. Western Blot analysis of WDR5 expression in Hs 181.Tes.

H00011091-M01A

WDR5 monoclonal antibody (M01), clone 2C2. Western Blot analysis of WDR5 expression in A-549 ( Cat # L025V1 ).

H00011091-M01A

Western blot was performed on whole cell lysates from mouse fibroblasts ( Lane 1, NIH/3T3 ) and embryonic stem cells ( Lane 2, E14Tg2a ) with Wdr5 polyclonal antibody ( Cat # PAB14068 ), diluted 1 : 1,000 in BSA/PBS-Tween.

PAB14068

ELISA was performed using a serial dilution of Wdr5 polyclonal antibody ( Cat # PAB14068 ). By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1 : 12,300.

PAB14068

Recombinant human WDR5, 1-334 aa, His-tagged

Mouse Pancreas; WB Suggested Anti-Wdr5 Antibody. . Titration: 1.0 ug/ml. . Positive Control: Mouse Pancreas; Wdr5 antibody - C-terminal region (ARP41307_P050) in Mouse Pancreas cells using Western Blot

ARP41307_P050

WDR5 Antibody (N-term) (AP54548PU-N) western blot analysis in A2058 cell line lysates (35 µg/lane). This demonstrates the WDR5 antibody detected the WDR5 protein (arrow).

AP54548PU-N

Western blot analysis using WDR5 mouse mAb AM06480SU-N against Hela (1) cell lysate.

AM06480SU-N

Western blot analysis of WDR5 in 293 cell lysate with WDR5 antibody AP55430PU-N at 1 µg/ml in (A) the absence and (B) the presence of blocking peptide.

AP55430CP-N

TP300162

TP316218

Western blot analysis of WDR5 in 293 cell lysate with WDR5 antibody at 1 µg/ml in (A) the absence and (B) the presence of blocking peptide.

TA319087

Immunocytochemistry of WDR5 in 293 cells with WDR5 antibody at 5 µg/mL.

TA319087

Immunocytochemistry of 293 cells with WDR5 antibody at 5ug/ml.

TA319087

Western blot analysis of WDR5 in 293 cell lysate with WDR5 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.

TA319832

Immunofluorescence of WDR5 in 293 cells with WDR5 antibody at 20 ug/mL.

TA319832

Immunocytochemistry of WDR5 in 293 cells with WDR5 antibody at 5 ug/mL.

TA319832

Western blot analysis of extracts of various cell lines, using WDR5 antibody.

TA332503

WB Suggested Anti-WDR5 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: 721_B cell lysate

TA345998

Sample Type: Human brain stem cells (NT2) Primary Antibody Dilution: 1: 500; Secondary Antibody: Goat anti-rabbit Alexa Fluor 594; Secondary Antibody: ilution: 1: 1000; Color/Signal Descriptions: Red: WDR5 Blue: DAPI; Gene Name: WDR5; Submitted by: Dr. Yuzhi Chen, University of Arkansas for Medical Science

TA345998

Amount and Sample Type: 500 ug Human NT2 cell lysate; Amount of IP Antibody: 6 ug; Primary Antibody: WDR5; Primary Antibody Dilution: 1: 500; Secondary Antibody: Goat anti-rabbit Alexa-Fluor 594; Secondary Antibody Dilution: 1: 5000; Gene Name: WDR5; Submitted by: Dr. Yuzhi Chen, University of Arkansas for Medical Science

TA345998

WB was performed using the WDR5 ab at 1:1000 in TBS-Tween containing with 5% skimmed milk on the purified recombinant protein (lanes 1-3), ectopically expressed FLAG-tagged WDR5 (WDR5-F) which was first IP'd with anti-FLAG (lane 4) and nuclear extracts from HeLa (lane 6) and HEK293 (lane 7). Lane 5 (MOCK) represents a negative control. B: WB was performed using nuclear extracts from HeLa (HeLa NE, 20 ug) and the WDR5 ab at 1:1,000 in TBS-Tween with 5% skimmed milk.

TA347311

WB was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the antibody against mouse Wdr5, diluted 1:1,000 in BSA/PBS-Tween. The molecular weight marker (M, in kDa) is shown on the left; the location of the protein of interest (36 kDa) is indicated on the right.

TA347312

Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody against mouse Wdr5. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:12,300.

TA347312

WB using the antibody against WDR5 diluted of 1:1000 in TBS-Tween containing 5% skimmed milk (lane 2). The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right.

TA347313

Primary Antibodies

Catalog No.	Host	Iso.	Clone	Pres.	React.	Applications
TA319832	WDR5 / BIG3 (N-term) antibody
	Rabbit Polyclonal WDR5 Antibody
	Rabbit	IgG		Aff - Purified	Hu	E, ICC/IF, WB	0.1 mg / €371.00
							OriGene Technologies, Inc.
+2 additional images
TA332503	WDR5 / BIG3 antibody
	Rabbit anti-WDR5 Polyclonal Antibody
	Rabbit	IgG		Purified	Hu, Ms, Rt		0.1 mg / €335.00
							OriGene Technologies, Inc.
TA345998	WDR5 / BIG3 antibody
	Rabbit Polyclonal Anti-WDR5 Antibody
	Rabbit	IgG		Purified	Bov, Can, Eq, GP, Hu, Ms, Por, Rt, Ye, Ze	IP, P, WB	50 µg / €360.00
							OriGene Technologies, Inc.
+2 additional images
TA347311	WDR5 / BIG3 antibody
	Rabbit Polyclonal WDR5 Antibody
	Rabbit	IgG		Purified	Hu		0.1 ml / €335.00
							OriGene Technologies, Inc.
TA347312	WDR5 / BIG3 antibody
	Rabbit Polyclonal WDR5 Antibody
	Rabbit	IgG		Purified	Ms	E	0.1 ml / €335.00
							OriGene Technologies, Inc.
+1 additional image
TA347313	WDR5 / BIG3 antibody
	Rabbit Polyclonal WDR5 Antibody
	Rabbit	IgG		Purified	Hu		50 µg / €335.00
							OriGene Technologies, Inc.
TA350617	WDR5 / BIG3 antibody
	Rabbit Polyclonal Anti-WDR5 Antibody
	Rabbit	IgG		Purified	Hu, Ms, Rt	E, WB	0.1 ml / €335.00
							OriGene Technologies, Inc.
AM06480SU-N	WDR5 / BIG3 antibody
	Mouse	IgG2b	7B11	Ascites	Hu	E, WB	0.1 ml / €400.00
							Acris Antibodies GmbH
AP54548PU-N	WDR5 / BIG3 (N-term) antibody
	Rabbit	Ig		Aff - Purified	Hu	WB	0.4 ml / €370.00
							Acris Antibodies GmbH

CRISPR

Catalog No.	Species	Donor Vector
KN200162	WDR5 - human gene knockout kit via CRISPR
	Human	GFP-Puro	1 kit / €1,290.00
			OriGene Technologies, Inc.
KN200162BN	WDR5 - human gene knockout kit via CRISPR
	Human	mBFP-Neo	1 kit / €1,290.00
			OriGene Technologies, Inc.
KN200162LP	WDR5 - human gene knockout kit via CRISPR
	Human	Luciferase-Puro	1 kit / €1,290.00
			OriGene Technologies, Inc.
KN200162RB	WDR5 - human gene knockout kit via CRISPR
	Human	RFP-BSD	1 kit / €1,290.00
			OriGene Technologies, Inc.
KN319366	Wdr5 - mouse gene knockout kit via CRISPR
	Mouse	GFP-Puro	1 kit / €1,290.00
			OriGene Technologies, Inc.
KN319366BN	Wdr5 - mouse gene knockout kit via CRISPR
	Mouse	mBFP-Neo	1 kit / €1,290.00
			OriGene Technologies, Inc.
KN319366LP	Wdr5 - mouse gene knockout kit via CRISPR
	Mouse	Luciferase-Puro	1 kit / €1,290.00
			OriGene Technologies, Inc.
KN319366RB	Wdr5 - mouse gene knockout kit via CRISPR
	Mouse	RFP-BSD	1 kit / €1,290.00
			OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No.	Species	Pres.	Purity	Source
TP300162	WDR5 / BIG3 (transcript variant 2)
	Recombinant protein of human WD repeat domain 5 (WDR5), transcript variant 2
	Human		> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €438.00
					OriGene Technologies, Inc.

TP316218	WDR5 / BIG3 (transcript variant 1)
	Recombinant protein of human WD repeat domain 5 (WDR5), transcript variant 1
	Human		> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €438.00
					OriGene Technologies, Inc.

AR09569PU-L	WDR5 / BIG3 (1-334, His-tag)
	Human	Purified	> 95 % by SDS – PAGE	E. coli	0.5 mg / €750.00
					Acris Antibodies GmbH
+1 additional image

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