WEE1 / Wee1-like protein kinase antibody

Principal name

WEE1 / Wee1-like protein kinase antibody

Alternative names for WEE1 / Wee1-like protein kinase antibody

Wee1A kinase, WEE1hu

SwissProt ID

P30291 (Human), P47810 (Mouse), Q63802 (Rat)

Gene ID

7465 (WEE1), 22390 (Wee1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit, Mouse

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C)

Background of WEE1 / Wee1-like protein kinase antibody

Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

WEE1 / Wee1-like protein kinase antibody

Western blot analysis of extracts from 293 cells, using WEE1 (Ab-53) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

WEE1 / Wee1-like protein kinase Control Peptide

WEE1 / Wee1-like protein kinase Purified
50 µg / €190.00
  Acris Antibodies GmbH

WEE1 / Wee1-like protein kinase antibody

Western blot analysis of Wee 1 expression in mouse small intestine tissue lysate. Rabbit Aff - Purified Hu, Ms, Rt IP, WB
0.1 mg / €390.00
  Acris Antibodies GmbH

WEE1 / Wee1-like protein kinase pSer123 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu P
0.4 ml / €370.00
  Acris Antibodies GmbH

WEE1 / Wee1-like protein kinase (C-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

WEE1 / Wee1-like protein kinase (Center) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

WEE1 / Wee1-like protein kinase pSer642 antibody

Western blot analysis of extracts from 293 cells treated with etoposide using WEE1 (Phospho-Ser642) Antibody AP55734PU-N.The lane on the right is treated with the antigen-specific peptide. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 ml / €340.00
  Acris Antibodies GmbH

WEE1 / Wee1-like protein kinase pSer642 antibody

Western blot analysis of extracts from 293 cells treated with etoposide using WEE1 (Phospho-Ser642) Antibody AP55734PU-N.The lane on the right is treated with the antigen-specific peptide. Rabbit Aff - Purified Hu, Ms, Rt WB
50 µl / €270.00
  Acris Antibodies GmbH

WEE1 / Wee1-like protein kinase antibody

WEE1 / Wee1-like protein kinase Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

WEE1 / Wee1-like protein kinase antibody

Immunofluorescence of purified MaxPab antibody to WEE1 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

WEE1 / Wee1-like protein kinase antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between WEE1 and FBXW11. HeLa cells were stained with anti-WEE1 rabbit purified polyclonal 1:1200 and anti-FBXW11 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

WEE1 / Wee1-like protein kinase antibody

WEE1 monoclonal antibody (M01A), clone 5B6 Western Blot analysis of WEE1 expression in Hela NE ( Cat # L013V3 ). Mouse IgM 5B6 Ascites Hu, Ms, Rt E, WB
0.2 ml / €370.00
  Abnova Taiwan Corp.

WEE1 / Wee1-like protein kinase pSer123 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with WEE1 (phospho S123) polyclonal antibody ( Cat # PAB0632 ) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma Rabbit Hu E, P, WB
0.4 ml / €400.00
  Abnova Taiwan Corp.

WEE1 / Wee1-like protein kinase antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with WEE1 polyclonal antibody ( Cat # PAB3321 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma. Rabbit Hu E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

WEE1 / Wee1-like protein kinase antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with WEE1 polyclonal antibody ( Cat # PAB3322 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma. Rabbit Hu, Ms E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

WEE1 / Wee1-like protein kinase antibody

Western blot analysis of WEE1 expression in mouse small intestine tissue lysate. Rabbit Hu, Ms, Rt IP, WB
0.1 mg / €470.00
  Abnova Taiwan Corp.

WEE1 / Wee1-like protein kinase pSer642 antibody

Western blot analysis of extracts from 293 cells treated with etoposide 25uM 60', using WEE1 (Phospho-Ser642) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech

WEE1 / Wee1-like protein kinase pSer642 antibody

Western blot analysis of extracts from 293 cells treated with etoposide 25uM 60', using WEE1 (Phospho-Ser642) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €310.00
  Assay Biotech

WEE1 / Wee1-like protein kinase pSer53 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using WEE1 (Phospho-Ser53) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P
50 µg / €205.00
  Assay Biotech

WEE1 / Wee1-like protein kinase pSer53 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using WEE1 (Phospho-Ser53) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P
0.1 mg / €310.00
  Assay Biotech

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