WFDC2 / WAP5 antibody

Principal name

WFDC2 / WAP5 antibody

Alternative names for WFDC2 / WAP5 antibody

HE4, WAP four-disulfide core domain protein 2, Major epididymis-specific protein E4, Epididymal secretory protein E4, Putative protease inhibitor WAP5

SwissProt ID

Q14508 (Human), Q8MI69 (Pig), Q9DAU7 (Mouse)

Gene ID

10406 (WFDC2)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Dot blot (Dot), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Luminex (LMNX)

Background of WFDC2 / WAP5 antibody

The whey acidic protein (WAP) domain is a conserved motif, containing eight cysteines found in a characteristic 4-disulphide core arrangement, that is present in a number of otherwise unrelated proteins. One of these proteins, which contains two WAP domains, is HE4 (also known as WFDC2), originally described as an epididymis specific protein but more recently suggested to be a putative serum tumour marker for ovarian cancer and a presumptive role in natural immunity. The HE4 protein expression is not only confined to epidydimis but is expressed in a number of normal human tissues out side the reproductive system, including regions of the respiratory tract and nasopharynx and in a subset of lung tumor cell lines. HE4 gene expression was highest in normal human trachea and salivary gland, and to a lesser extent, lung, prostate, pituitary gland, thyroid, and kidney. Highest level of expression of ESPE4 was observed in adenocarcinomas of the lung, and occasional breast, transitional cell and pancreatic carcinomas (1). The WFDC2 gene under goes extensive splicing in malignant tissues that give rise to five WAP domain containing iso forms (2).
WFDC2 is expressed in some epithelial cells of the upper airways as well as in mucous cells and ducts of submucosal glands. No staining was seen in peripheral lung. Intense staining is found in major salivary glands and in minor glands of the nose, sinuses, posterior tongue and tonsil. Studies with the related protein Secretory Leukocyte Protease Inhibitor (SLPI) show that although both proteins are expressed in similar tissues, the precise cellular localization differs. Significant increases in expression and localization of WFDC2 are seen in patients with Cystic Fibrosis (3). ESPE2 protien is a 124 amino acids (15 kDa) protein. ESPE4 protein has sequence homology to extracellular proteinase inhibitor and is localized on human chromosome 20. The ESPE4 protein is a secreted glycoprotein (Asn-44) that is over expressed in serous and endometrioid ovarian carcinomas (4). There are at least 5 named variants (HE4-V1 to HE4-V5) expressed as a result of alternate splicing and they serve as endopeptidase inhibitors.

General readings

1. Huhtinen K, Suvitie P, Hiissa J, Junnila J, Huvila J, Kujari H, Setälä M, Härkki P, Jalkanen J, Fraser J, Mäkinen J, Auranen A, Poutanen M, Perheentupa A. Serum HE4 concentration differentiates malignant ovarian tumours from ovarian endometriotic cysts. British journal of cancer 2009 Apr 100 (8): 1315-9.
PMID: 19337252

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

WFDC2 control peptide

WFDC2
0.25 mg / €250.00
  Acris Antibodies GmbH

WFDC2 control peptide

WFDC2
0.25 mg / €250.00
  Acris Antibodies GmbH

WFDC2 / WAP5 antibody

Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-WFDC2 mouse monoclonal antibody. (UM800018; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min) Mouse IgG1 UMAB87 Purified Hu P
0.1 ml / €468.00
  OriGene Technologies, Inc.

UltraMAB

WFDC2 / WAP5 antibody

Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-WFDC2 mouse monoclonal antibody. (UM800019; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min) Mouse IgG1 UMAB88 Purified Hu P
0.1 ml / €468.00
  OriGene Technologies, Inc.

UltraMAB

WFDC2 / WAP5 antibody

Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-WFDC2 mouse monoclonal antibody. (UM800018; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min) Mouse IgG1 UMAB87 Purified Hu P
30 µl / €140.00
  OriGene Technologies, Inc.

UltraMAB

WFDC2 / WAP5 antibody

Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-WFDC2 mouse monoclonal antibody. (UM800019; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min) Mouse IgG1 UMAB88 Purified Hu P
30 µl / €140.00
  OriGene Technologies, Inc.

UltraMAB

WFDC2 / WAP5 antibody

WFDC2 (HE4)  Elisa with 1E12 Capture (TA600329) and 2A7 Detection (TA700330) Antibodies. Substrate used: Recombinant Human WFDC2 (TP308491) Mouse IgG1 OTI1E12 Purified Hu E, LMNX
0.1 mg / €325.00
  OriGene Technologies, Inc.

WFDC2 / WAP5 antibody

WFDC2 (HE4)  Elisa with 3A9 Capture (TA600332) and 2A7 Detection (TA700330) Antibodies. Substrate used: Recombinant Human WFDC2 (TP308491) Mouse IgG1 OTI3A9 Purified Hu E, LMNX
0.1 mg / €325.00
  OriGene Technologies, Inc.

WFDC2 / WAP5 antibody

WFDC2 (HE4)  Elisa with 1G7 Capture (TA600333) and 2A7 Detection (TA700330) Antibodies. Substrate used: Recombinant Human WFDC2 (TP308491) Mouse IgG1 OTI1G7 Purified Hu E, LMNX
0.1 mg / €325.00
  OriGene Technologies, Inc.

WFDC2 / WAP5 antibody

WFDC2 (HE4)  Elisa with 3A9 Capture (TA600332) and 1B9 Detection (TA700329) Antibodies. Substrate used: Recombinant Human WFDC2 (TP308491) Mouse IgG1 OTI1B9 Purified Hu E, LMNX
50 µg / €345.00
  OriGene Technologies, Inc.

WFDC2 / WAP5 antibody

WFDC2 (HE4)  Elisa with 3A9 Capture (TA600332) and 2A7 Detection (TA700330) Antibodies. Substrate used: Recombinant Human WFDC2 (TP308491) Mouse IgG1 OTI2A7 Purified Hu E, LMNX
50 µg / €345.00
  OriGene Technologies, Inc.

WFDC2 / WAP5 antibody

WFDC2 (HE4)  Elisa with 2A7 Capture (TA600331) and 1E12 Detection (TA700331) Antibodies. Substrate used: Recombinant Human WFDC2 (TP308491) Mouse IgG1 OTI1E12 Purified Hu E, LMNX
50 µg / €345.00
  OriGene Technologies, Inc.

WFDC2 / WAP5 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-WFDC2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801136) Mouse IgG1 OTI1E12 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

WFDC2 / WAP5 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-WFDC2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801137) Mouse IgG1 OTI1B9 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

WFDC2 / WAP5 antibody

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-WFDC2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801273) Mouse IgG1 OTI2A7 Purified Hu P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

WFDC2 / WAP5 (29-43) antibody

HEK293 lysate (10 µg protein in RIPA buffer) overexpressing Human WFDC2 with C-terminal MYC tag probed with AP22455PU-N  (1 µg/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with AP22455PU-N (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence. Goat Aff - Purified Can, Hu E, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

WFDC2 / WAP5 antibody

Western blot analysis of WFDC2 in A549 cell lysate with WFDC2 antibody at  1 ug/ml in (A) the absence and (B) the presence of blocking peptide. Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

WFDC2 / WAP5 antibody

WFDC2 (HE4)  Elisa with 1B9 Capture (TA600330) and 1E12 Detection (TA700331) Antibodies. Substrate used: Recombinant Human WFDC2 (TP308491) Mouse IgG1 OTI1B9 Purified Hu E, LMNX
0.1 mg / €325.00
  OriGene Technologies, Inc.

WFDC2 / WAP5 antibody

WFDC2 (HE4)  Elisa with 2A7 Capture (TA600331) and 1E12 Detection (TA700331) Antibodies. Substrate used: Recombinant Human WFDC2 (TP308491) Mouse IgG1 OTI2A7 Purified Hu E, LMNX
0.1 mg / €325.00
  OriGene Technologies, Inc.

WFDC2 / WAP5 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY WFDC2 (RC208491, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-WFDC2. Mouse IgG1 OTI3A9 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Items 1 to 20 of 32 total

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