WIF1 antibody

Principal name

WIF1 antibody

Alternative names for WIF1 antibody

WIF-1, UNQ191/PRO217, Wnt inhibitory factor 1

SwissProt ID

Q6IN38 (Rat), Q9W6F8 (Xenla), Q9W6F9 (Danre), Q9WUA1 (Mouse), Q9Y5W5 (Human)

Gene ID

11197 (WIF1), 24117 (Wif1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Mouse, Rabbit, Rat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of WIF1 antibody

WIF-1 (Wnt Inhibitory Factor 1) is a secreted protein that binds to Wnt proteins and inhibits their activity. In situ hybridization analysis in Xenopus laevis and zebrafish indicate that the message is highly expressed in presomitic mesoderm, the notochord, anterior regions of the brain, branchial arches, nasal placodes, and otic vescicles. WIF-1 inhibits secondary axis induction by Wnts and promotes secondary axis induction by Chordin in Xenopus embryos. In vitro, WIF-1 binds to Drosophila Wingless and Xenopus Wnt8 proteins. WIF-1 is implicated as an early event tumor suppressor in cancers of the prostate, breast, lung, and bladder, while in other cancer types, such as colon adenocarcinoma, WIF-1 facilitates tumorigenesis. WIF-1 is also expressed in mammalian retina and may play a role in photoreceptor development. The protein is synthesized as a 379 amino acid (aa) molecule that contains a signal sequence, a 150 aa WIF domain, 5 EGF-like repeats, and a hydrophilic domain at the carboxy terminus.

General readings

Park JH., et al. (2014) Dev. Biol. 386(1):227-236.Stock M., et al. (2013) Arthritis Rheum. 65(9):2310-2322.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

WIF1 (N-term) antibody

  Rabbit Polyclonal Antibody against WIF1 (N-term)  
Western blot analysis of WIF1 (arrow) using rabbit polyclonal WIF1 Antibody (N-term) (Cat.#TA302106). Rabbit Ig Aff - Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

WIF1 (C-term) antibody

  Rabbit Polyclonal Antibody against WIF1 (C-term)  
Western blot analysis of WIF1 (arrow) using rabbit polyclonal WIF1 (C-term) Antibody (Cat.#TA302107). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the WIF1 gene (Lane 2) (Origene Technologies). Rabbit Ig Aff - Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

WIF1 antibody

  Rabbit anti-WIF1 Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using WIF1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

WIF1 antibody

  Rabbit Polyclonal Anti-WIF1 Antibody  
WB Suggested Anti-WIF1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human Muscle Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

WIF1 antibody

Immunohistochemical analysis of paraffin-embedded ovary tumour tissues (left) and lung cancer (right) using WIF1 antibody Cat.-No AM06523SU-N with DAB staining. Mouse IgG1 1G5 Ascites Hu E, ICC/IF, P, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

WIF1 antibody

WIF1 Mouse IgG1 6D27 Purified Hu P, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

WIF1 antibody

WIF1 Rat IgG2 3M51 Purified Ms WB
0.1 mg / €440.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

WIF1 - human gene knockout kit via CRISPR

WIF1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

WIF1 - human gene knockout kit via CRISPR

WIF1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

WIF1 - human gene knockout kit via CRISPR

WIF1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

WIF1 - human gene knockout kit via CRISPR

WIF1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Wif1 - mouse gene knockout kit via CRISPR

Wif1 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

WIF1

  Recombinant protein of human WNT inhibitory factor 1 (WIF1)  
WIF1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

WIF1

  Purified recombinant protein of Human WNT inhibitory factor 1 (WIF1)  
WIF1 Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
10 µg / €139.00
  OriGene Technologies, Inc.

WIF1 (29-379, His-tag)

WIF1 Human Purified > 90 % by SDS - PAGE Insect cells
0.25 mg / €990.00
  Acris Antibodies GmbH

WIF1 (29-379, His-tag)

WIF1 Human Purified > 90 % by SDS - PAGE Insect cells
50 µg / €370.00
  Acris Antibodies GmbH

WIF1 (29-379, His-tag)

WIF1 Mouse Purified > 90 % by SDS - PAGE Insect cells
0.25 mg / €940.00
  Acris Antibodies GmbH

WIF1 (29-379, His-tag)

WIF1 Mouse Purified > 90 % by SDS - PAGE Insect cells
50 µg / €300.00
  Acris Antibodies GmbH

Lysates

Catalog No.    

WIF1 overexpression lysate

WIF1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, WIF1 (mGFP-tagged) - Human WNT inhibitory factor 1 (WIF1), 200 uL, >10^7 TU/mL

Lenti ORF particles, WIF1 (mGFP-tagged) - Human WNT inhibitory factor 1 (WIF1), 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

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