WIF1 antibody

Principal name

WIF1 antibody

Alternative names for WIF1 antibody

WIF-1, UNQ191/PRO217, Wnt inhibitory factor 1

SwissProt ID

Q6IN38 (Rat), Q9W6F8 (Xenla), Q9W6F9 (Danre), Q9WUA1 (Mouse), Q9Y5W5 (Human)

Gene ID

11197 (WIF1), 24117 (Wif1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Xen (Xenopus), Ze (Zebrafish), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Mouse, Rabbit, Rat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C)

Background of WIF1 antibody

WIF-1 (Wnt Inhibitory Factor 1) is a secreted protein that binds to Wnt proteins and inhibits their activity. In situ hybridization analysis in Xenopus laevis and zebrafish indicate that the message is highly expressed in presomitic mesoderm, the notochord, anterior regions of the brain, branchial arches, nasal placodes, and otic vescicles. WIF-1 inhibits secondary axis induction by Wnts and promotes secondary axis induction by Chordin in Xenopus embryos. In vitro, WIF-1 binds to Drosophila Wingless and Xenopus Wnt8 proteins. WIF-1 is implicated as an early event tumor suppressor in cancers of the prostate, breast, lung, and bladder, while in other cancer types, such as colon adenocarcinoma, WIF-1 facilitates tumorigenesis. WIF-1 is also expressed in mammalian retina and may play a role in photoreceptor development. The protein is synthesized as a 379 amino acid (aa) molecule that contains a signal sequence, a 150 aa WIF domain, 5 EGF-like repeats, and a hydrophilic domain at the carboxy terminus.

General readings

Park JH., et al. (2014) Dev. Biol. 386(1):227-236.Stock M., et al. (2013) Arthritis Rheum. 65(9):2310-2322.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

WIF1 (N-term) antibody

Western blot analysis of WIF1 (arrow) using rabbit polyclonal WIF1 Antibody (N-term) (Cat.#TA302106). Rabbit Ig Aff - Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

WIF1 (C-term) antibody

Western blot analysis of WIF1 (arrow) using rabbit polyclonal WIF1 (C-term) Antibody (Cat.#TA302107). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the WIF1 gene (Lane 2) (Origene Technologies). Rabbit Ig Aff - Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

WIF1 antibody

Western blot analysis of extracts of various cell lines, using WIF1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

WIF1 antibody

WB Suggested Anti-WIF1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human Muscle Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

WIF1 antibody

Immunohistochemical analysis of paraffin-embedded ovary tumour tissues (left) and lung cancer (right) using WIF1 antibody Cat.-No AM06523SU-N with DAB staining. Mouse IgG1 1G5 Ascites Hu E, ICC/IF, P, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

WIF1 antibody

WIF1 Mouse IgG1 6D27 Purified Hu P, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

WIF1 antibody

WIF1 Rat IgG2 3M51 Purified Ms WB
0.1 mg / €440.00
  Acris Antibodies GmbH

WIF1 antibody

WIF1 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

WIF1 antibody

Western Blot analysis of WIF1 expression in transfected 293T cell line (H00011197-T01) by WIF1 MaxPab polyclonal antibody.

Lane 1: WIF1 transfected lysate(41.69 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

WIF1 antibody

Western Blot analysis of WIF1 expression in transfected 293T cell line (H00011197-T01) by WIF1 MaxPab polyclonal antibody.

Lane 1: WIF1 transfected lysate(41.50 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

WIF1 antibody

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with WIF1 polyclonal antibody ( Cat # PAB2563 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. Rabbit Hu E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

WIF1 antibody

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with WIF1 polyclonal antibody ( Cat # PAB2564 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. Rabbit Hu E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

WIF1 (N-term) antibody

Human Muscle; WB Suggested Anti-WIF1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Muscle; WIF1 antibody - N-terminal region (ARP52298_P050) in Human Muscle cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

WIF1 antibody

WIF1 Rat IgG2 RM0144-3M51 Purified Ms WB
0.1 mg / €440.00
  Novus Biologicals Inc.

WIF1 antibody

Immunohistochemistry-Paraffin: WIF1 Antibody (1G5) [NBP1-51658] - Immunohistochemical analysis of paraffin-embedded ovary tumour tissues (left) and lung cancer (right) using WIF1 mouse mAb with DAB staining. Mouse IgG1 1G5 Purified Hu E, ICC/IF, P, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

WIF1 antibody

WIF1 Rabbit Aff - Purified Hu WB
50 µg / €440.00
  Novus Biologicals Inc.

WIF1 antibody

Immunohistochemistry-Paraffin: WIF1 Antibody [NBP1-86251] Immunohistochemical staining of human duodenum shows strong cytoplasmic and membranous positivity in glandular cells. Rabbit IgG Aff - Purified Hu P
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

WIF1

WIF1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

WIF1

WIF1 Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
10 µg / €139.00
  OriGene Technologies, Inc.

WIF1 (29-379, His-tag)

WIF1 Human Purified > 90 % by SDS - PAGE Insect cells
0.25 mg / €990.00
  Acris Antibodies GmbH

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