WNK1 antibody

Principal name

WNK1 antibody

Alternative names for WNK1 antibody

KDP, KIAA0344, PRKWNK1, p65, Serine/threonine-protein kinase WNK1, Protein kinase with no lysine 1, Protein kinase lysine-deficient 1, Kinase deficient protein, Erythrocyte 65 kDa protein

SwissProt ID

P83741 (Mouse), Q9H4A3 (Human), Q9JIH7 (Rat)

Gene ID

65125 (WNK1), 116477 (Wnk1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Por (Porcine)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Immunoprecipitation (IP), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), Frozen Sections (C)

Background of WNK1 antibody

The protein kinase superfamily contains over a thousand proteins in 57 subfamilies that all share a catalytic core of 250 - 300 amino acids organized in two domains. WNK kinases (With No lysine (K)) are serine-threonine protein kinases that contain a cysteine residue in place of a lysine residue in a family of proteins that traditionally contain a lysine following a short string of hydro-phobic residues. WNK kinases contain a lysine upstream of the traditional position, within a glycine string. This lysine functions as an anchor and orients ATP through interactions with the alpha and beta phosphoryl groups. The catalytic domains of WNK2, WNK3 and WNK4 are 95% homologous to WNK1. Human WNK1 maps to chromosome 12p13 and encodes a 2382 protein that is primarily expressed in heart, kidney, muscle and distal nephron. Human WNK3 maps to chromosome Xp11.21-p11.23 and encodes a protein that is primarily expressed in brain. Human WNK4 maps to chromosome 17q21-q22 and encodes a 1243 amino acid protein that is expressed in kidney. Aberrant function of WNK kinases and their associated signaling pathways are implicated in hypertension, increased renal salt reabsorption, and impaired K+ and H+ excretion.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

WNK1 antibody

Western Blot: WNK1 antibody - Detection of the short form of WNK1 in F-11 cell lysate. Rabbit Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

WNK1 antibody

Western blot analysis of WNK1 expression in EGF treated 293 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

WNK1 antibody

Western blot analysis of extracts of various cell lines, using WNK1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

WNK1 antibody

Anti-WNK1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Aff - Purified Hu P
50 µg / €379.00
  OriGene Technologies, Inc.

WNK1 antibody

Anti-WNK1 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Purified Hu
50 µg / €379.00
  OriGene Technologies, Inc.

WNK1 antibody

ELISA: HSN2 Antibody Rabbit IgG Purified Hu E
50 µg / €325.00
  OriGene Technologies, Inc.

WNK1 antibody

Immunoblot against extracts of COS cells transiently transfected with Myc-tagged WNK1, Myc-tagged WNK3 or untagged Kv2.1 plasmid probed with N391/68 (left) or 19E10 (right) TC supe. Mouse IgG2b N391/68 Supernatant Rt C, ICC/IF, WB
5 ml / €320.00
  Antibodies Incorporated

NeuroMab

WNK1 antibody

Immunohistochemistry (IHC) analyzes of WNK1 antibody (Cat.-No.: AP06363PU-N) in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

WNK1 (Internal) antibody

Skeletal muscle: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Bear, Bov, Bt, Chk, Elephant, Eq, Hst, Hu, Mky, Ms, Op, Platypus, Prim, Rb, Rt, Tr, Xen E, P
50 µg / €470.00
  Acris Antibodies GmbH

WNK1 antibody

Western Blot analysis of WNK1 expression in transfected 293T cell line (H00065125-T02) by WNK1 MaxPab polyclonal antibody.

Lane 1: WNK1 transfected lysate(42.40 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

WNK1 antibody

WNK1 Rabbit Hu, Ms, Rt WB
0.1 ml / €470.00
  Abnova Taiwan Corp.

WNK1 pThr58 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using WNK1 (Phospho-Thr58) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €205.00
  Assay Biotech

WNK1 pThr58 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using WNK1 (Phospho-Thr58) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €310.00
  Assay Biotech

WNK1 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using WNK1 (Ab-58) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €205.00
  Assay Biotech

WNK1 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using WNK1 (Ab-58) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €310.00
  Assay Biotech

WNK1 pThr60 antibody

Immunohistochemical analysis of p65 (pT60) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Ms, Por, Rt P, WB
30 µl / €210.00
  Cohesion Biosciences Ltd.

WNK1 pThr60 antibody

Immunohistochemical analysis of p65 (pT60) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Ms, Por, Rt P, WB
0.1 ml / €330.00
  Cohesion Biosciences Ltd.

WNK1 pThr60 antibody

Immunohistochemical analysis of p65 (pT60) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Ms, Por, Rt P, WB
0.2 ml / €530.00
  Cohesion Biosciences Ltd.

WNK1 antibody

Immunohistochemical analysis of p65 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Ms, Por, Rt P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

WNK1 antibody

Immunohistochemical analysis of p65 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Ms, Por, Rt P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

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