WNT3 antibody

Principal name

WNT3 antibody

Alternative names for WNT3 antibody

Proto-oncogene protein Wnt-3, INT4, INT-4

SwissProt ID

P17553 (Mouse), P31284 (Xenla), P56703 (Human)

Gene ID

7473 (WNT3)

Available reactivities

Hu (Human), Ms (Mouse), Can (Canine), Rt (Rat), Ze (Zebrafish)

Available hosts

Goat, Rabbit, Mouse

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Frozen Sections (C)

Background of WNT3 antibody

Wnts have diverse roles in governing cell fate, proliferation, migration, and polarity during development, particularly in embryogenesis and carcinogenesis. WNTs are transduced through at least three distinct intracellular signaling pathways, including the canonical Wnt/b-catenin, Wnt/Ca2+ and Wnt polarity pathways. Distinct sets of Wnt and Frizzled ligand-receptor pairs activate each of these pathways and lead to unique cellular responses. Wnt3 is expressed in embryonic mouse brain (with peak expression at around day 12 of gestation) and at low levels in the adult.
It is present in the cerebellum during mouse development, and is restricted to the Purkinje cell layer in the adult mouse. Wnt3 is required for normal limb development and plays an important role in the establishment of the apical ectodermal ridge. In motorneurons, Wnt3 is a retrograde signal that controls terminal branching of muscle afferents. Wnt3 is highly expressed in human cancer cell lines including A549 (lung cancer), MKN45 (gastric cancer) and MCF7 (breast cancer).
Wnt3 and Wnt3a are nearly 90% identical at the amino acid level.

General readings

Schmitt AM, Shi J, Wolf AM, Lu CC, King LA, Zou Y. , Wnt-Ryk signalling mediates medial-lateral retinotectal topographic mapping. ,Nature. 2006 Jan 5;439(7072):31-7. , PMID: 16280981

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

WNT3 antibody

TA302636 (1µg/ml) staining of lysate of cell line HEK293 (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

WNT3 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-WNT3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801734) Mouse IgG1 OTI1C5 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

WNT3 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-WNT3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801867) Mouse IgG2a OTI1G7 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

WNT3 antibody

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-WNT3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801892) Mouse IgG2b OTIG5 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

WNT3 (315-329) antibody

Human Thyroid (formalin-fixed, paraffin-embedded) stained with WNT3 antibody AP22539PU-N at 3.75 µg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Goat Aff - Purified Amph, Bear, Bov, Bt, Can, Chk, Eq, Fi, Hu, Mky, Ms, Op, Platypus, Por, Prim, Rt, Tr E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

WNT3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY WNT3 (RC211115, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-WNT3. Mouse IgG1 OTI1D9 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

WNT3 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-WNT3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801736) Mouse IgG1 OTI1F6 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

WNT3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY WNT3 (RC211115, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-WNT3. Mouse IgG1 OTI3F5 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

WNT3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY WNT3 (RC211115, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-WNT3. Mouse IgG1 OTID4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

WNT3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY WNT3 (RC211115, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-WNT3. Mouse IgG2a OTIC7 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

WNT3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY WNT3 (RC211115, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-WNT3. Mouse IgG2a OTI2A4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

WNT3 antibody

Western Blot analysis of WNT3 expression in transfected 293T cell line (H00007473-T01) by WNT3 MaxPab polyclonal antibody.

Lane 1: WNT3 transfected lysate(39.60 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

WNT3 antibody

WNT3 polyclonal antibody ( Cat # PAB7032 ) ( 1 µg/ml ) staining of human brain lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu, Ms E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

WNT3 (Center) antibody

Human Brain; WB Suggested Anti-WNT3 Antibody Titration: 0.2-1 ug/ml. Positive Control: Human brain; WNT3 antibody - middle region (ARP49956_P050) in Human Brain cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

WNT3 antibody

Western Blot: WNT3 Antibody [NB100-40793] - NB100-40793 (1ug/ml) staining of Human Brain lysate (35ug protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat IgG Aff - Purified Hu, Ms E, ICC/IF, P, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

WNT3 antibody

Western Blot: WNT3 Antibody [NBP1-56996] - This Anti-WNT3 antibody was used in Western Blot of Fetal Brain    tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu WB
50 µg / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

WNT3

WNT3 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

WNT3

WNT3 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates (Overexpressed)

Catalog No.    

WNT3 overexpression lysate

WNT3 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

19 Item(s)

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