WNT3A antibody

Principal name

WNT3A antibody

Alternative names for WNT3A antibody

Protein Wnt-3a

SwissProt ID

P27467 (Mouse), P31285 (Xenla), P56704 (Human)

Gene ID

89780 (WNT3A), 22416 (Wnt3a)

Available reactivities

Hu (Human), Can (Canine), Chk (Chicken), Ms (Mouse), Ze (Zebrafish), African clawed frog, Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Rt (Rat)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)

Background of WNT3A antibody

Wnt-3a belongs to the Wnt family of signaling proteins that play a key role in maintaining the integrity of embryonic and adult tissues. Expression of Wnt-3a occurs primarily along the dorsal midline across overlapping regions of the Central Nervous System (CNS). Wnt-3a signaling is essential for various morphogenetic events including embryonic patterning, cell determination, cell proliferation, CNS development, and cytoskeletal formation. Like other members of this family, Wnt-3a contains a highly conserved lipid modified cysteine rich domain that is essential for cell signaling. During a biochemical process called the canonical Wnt pathway; Wnt family members bind to and activate seven-pass transmembrane receptors of the Frizzled family ultimately leading to the disruption of β-Catenein degradation. Intracellular accumulation of β-Catenin increases translocation of the protein into the nucleus where it binds to TCF/LEF transcription factors to promote gene expression. Lack of Wnt signaling disrupts transcriptional activation of tumor suppressor genes and has shown to result in neoplastic transformation, oncogenesis, and human degenerative diseases.

General readings

Baenziger C., et al. (2006). Cell 125:509-522Bourhis E., et al. (2010). J. Biol. Chem. 285:9172-9179

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

WNT3A antibody

  Anti-Human WNT-3a Rabbit Polyclonal Antibody  
This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma.  The recommended concentration is 0.125 ug/ml - 0.25 ug/ml with an overnight incubation at 4C.  An HRP-labeled polymer detection system was used with a DAB chromogen.  Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended.  Optimal concentrations and conditions may vary. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

WNT3A antibody

  Rabbit Polyclonal Anti-WNT3A Antibody - N-terminal region  
WB Suggested Anti-WNT3A Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human heart Rabbit IgG Purified Can, Eq, GP, Hu, Ms, Rb, Rt, Ze P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

WNT3A antibody

  Rabbit Polyclonal Anti-WNT3A Antibody  
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: Mouse lung tissue, Primary antibody: (WNT3A Antibody) at dilution 1/325, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes Rabbit IgG Purified Hu, Ms E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

WNT3A antibody

Paraffin embedded sections of human breast cancer tissue were incubated with anti-human Wnt3a (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB) Mouse IgG2a 3A6 Purified Hu E, P, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

WNT3A antibody

Paraffin embedded sections of human breast cancer tissue were incubated with anti-human Wnt3a (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB) Mouse IgG2a 3A6 Purified Hu E, P, WB
50 µl / €230.00
  Acris Antibodies GmbH

WNT3A antibody

WNT3A Rabbit Biotin Hu E, WB
50 µg / €340.00
  Acris Antibodies GmbH

WNT3A antibody

WNT3A Rabbit Biotin Hu E, WB
25 µg / €230.00
  Acris Antibodies GmbH

WNT3A antibody

This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 0.125 mg/ml-0.25 µg/ml with an overnight incubation at 4˚C.  An HRP-labeled polymer detection system was used with a DAB chromogen.  Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. Rabbit Aff - Purified Hu E, P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

WNT3A antibody

This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 0.125 mg/ml-0.25 µg/ml with an overnight incubation at 4˚C.  An HRP-labeled polymer detection system was used with a DAB chromogen.  Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. Rabbit Aff - Purified Hu E, P, WB
50 µg / €230.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

WNT3A - human gene knockout kit via CRISPR

WNT3A - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

WNT3A - human gene knockout kit via CRISPR

WNT3A - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

WNT3A - human gene knockout kit via CRISPR

WNT3A - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

WNT3A - human gene knockout kit via CRISPR

WNT3A - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Wnt3a - mouse gene knockout kit via CRISPR

Wnt3a - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

WNT3A

WNT3A Mouse Purified > 75 % pure by SDS-PAGE and visualised by silver stain Cell culture
10 µg / €330.00
  Acris Antibodies GmbH

WNT3A (19-352, )

WNT3A Human Purified > 85 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

WNT3A (19-352, )

WNT3A Human Purified > 85 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

Lysates

Catalog No.    

WNT3A overexpression lysate

WNT3A overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, WNT3A (mGFP-tagged) - Human wingless-type MMTV integration site family, member 3A (WNT3A), 200 uL, >10^7 TU/mL

Lenti ORF particles, WNT3A (mGFP-tagged) - Human wingless-type MMTV integration site family, member 3A (WNT3A), 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, WNT3A (Myc-DDK tagged) - Human wingless-type MMTV integration site family, member 3A (WNT3A) , 200 uL, >10^7 TU/mL

Lenti ORF particles, WNT3A (Myc-DDK tagged) - Human wingless-type MMTV integration site family, member 3A (WNT3A) , 200 uL, >10^7 TU/mL  
200 µl / €940.00
  OriGene Technologies, Inc.

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