WNT3A antibody

Principal name

WNT3A antibody

Alternative names for WNT3A antibody

Protein Wnt-3a

SwissProt ID

P27467 (Mouse), P31285 (Xenla), P56704 (Human)

Gene ID

89780 (WNT3A), 22416 (Wnt3a)

Available reactivities

Hu (Human), Can (Canine), Chk (Chicken), Ms (Mouse), Ze (Zebrafish), African clawed frog, Xen (Xenopus), Rt (Rat), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Rabbit, Mouse, Rat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Frozen Sections (C), Neutralisation (NEUT)

Background of WNT3A antibody

Wnt-3a belongs to the Wnt family of signaling proteins that play a key role in maintaining the integrity of embryonic and adult tissues. Expression of Wnt-3a occurs primarily along the dorsal midline across overlapping regions of the Central Nervous System (CNS). Wnt-3a signaling is essential for various morphogenetic events including embryonic patterning, cell determination, cell proliferation, CNS development, and cytoskeletal formation. Like other members of this family, Wnt-3a contains a highly conserved lipid modified cysteine rich domain that is essential for cell signaling. During a biochemical process called the canonical Wnt pathway; Wnt family members bind to and activate seven-pass transmembrane receptors of the Frizzled family ultimately leading to the disruption of β-Catenein degradation. Intracellular accumulation of β-Catenin increases translocation of the protein into the nucleus where it binds to TCF/LEF transcription factors to promote gene expression. Lack of Wnt signaling disrupts transcriptional activation of tumor suppressor genes and has shown to result in neoplastic transformation, oncogenesis, and human degenerative diseases.

General readings

Baenziger C., et al. (2006). Cell 125:509-522Bourhis E., et al. (2010). J. Biol. Chem. 285:9172-9179

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

WNT3A antibody

This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma.  The recommended concentration is 0.125 ug/ml - 0.25 ug/ml with an overnight incubation at 4C.  An HRP-labeled polymer detection system was used with a DAB chromogen.  Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended.  Optimal concentrations and conditions may vary. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

WNT3A antibody

WB Suggested Anti-WNT3A Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human heart Rabbit IgG Purified Can, Eq, GP, Hu, Ms, Rb, Rt, Ze P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

WNT3A antibody

Paraffin embedded sections of human breast cancer tissue were incubated with anti-human Wnt3a (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB) Mouse IgG2a 3A6 Purified Hu E, P, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

WNT3A antibody

Paraffin embedded sections of human breast cancer tissue were incubated with anti-human Wnt3a (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB) Mouse IgG2a 3A6 Purified Hu E, P, WB
50 µl / €230.00
  Acris Antibodies GmbH

WNT3A antibody

WNT3A Rabbit Biotin Hu E, WB
50 µg / €340.00
  Acris Antibodies GmbH

WNT3A antibody

WNT3A Rabbit Biotin Hu E, WB
25 µg / €230.00
  Acris Antibodies GmbH

WNT3A antibody

This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 0.125 mg/ml-0.25 µg/ml with an overnight incubation at 4?C.  An HRP-labeled polymer detection system was used with a DAB chromogen.  Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. Rabbit Aff - Purified Hu E, P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

WNT3A antibody

This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 0.125 mg/ml-0.25 µg/ml with an overnight incubation at 4?C.  An HRP-labeled polymer detection system was used with a DAB chromogen.  Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. Rabbit Aff - Purified Hu E, P, WB
50 µg / €230.00
  Acris Antibodies GmbH

WNT3A antibody

Immunofluorescence of purified MaxPab antibody to WNT3A on HeLa cell. [antibody concentration 10 ug/ml] Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

WNT3A antibody

Immunoprecipitation of WNT3A transfected lysate using anti-WNT3A MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with WNT3A purified MaxPab mouse polyclonal antibody (B01P) (H00089780-B01P). Rabbit Serum Hu E(detection), IP
0.1 ml / €380.00
  Abnova Taiwan Corp.

WNT3A antibody

Western Blot analysis of WNT3A expression in transfected 293T cell line (H00089780-T01) by WNT3A MaxPab polyclonal antibody.

Lane 1: WNT3A transfected lysate(42.90 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

WNT3A antibody

The lysate of A-549 ( 20 µg ) was resolved by SDS-PAGE and probed with WNT3A monoclonal antibody, clone 3A6 ( Cat # MAB2063 ) ( 1:1000 ). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG2a 3A6 Hu E, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

WNT3A antibody

Human Prostate; prostate; WNT3A antibody - N-terminal region (ARP58818_P050) in Human Prostate cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Can, Chk, Hu, Ms, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

WNT3A (C-term) antibody

Human Placenta; Immunohistochemistry with Placenta tissue at an antibody concentration of Dilution: 1:500 using anti-Wnt3a antibody (ARP58819_P050); Wnt3a antibody - C-terminal region (ARP58819_P050) in Human Placenta cells using Immunohistochemistry Rabbit Aff - Purified Hu, Ms WB
50 µg / €350.00
  AVIVA Systems Biology

WNT3A antibody

WNT3A Rabbit IgG Aff - Purified Hu, Ms WB
0.1 mg / €290.00
  Boster Immunoleader

WNT3A antibody

WNT3A Mouse IgG2a 3A6 Purified Hu E, P, SDS-PAGE, WB
0.1 ml / €380.00
  Novus Biologicals Inc.

WNT3A antibody

WNT3A Rabbit Aff - Purified Hu, Ms P, WB
50 µg / €440.00
  Novus Biologicals Inc.

WNT3A antibody

WNT3A Rabbit Aff - Purified Can, Chk, Hu, Ms, Xen, Ze P, WB
50 µg / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

WNT3A

WNT3A Mouse > 75 %
Preparation: .
Purity Detail: >75% as determined by SDS-PAGE and Coomassie blue staining.
Cell culture

Regular Price: 10 µg / €399.00

Special Price: 10 µg / €239.00

  OriGene Technologies, Inc.

WNT3A

WNT3A Mouse Purified > 75 % pure by SDS-PAGE and visualised by silver stain Cell culture
10 µg / €330.00
  Acris Antibodies GmbH

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