WT1 / Wilms tumor protein antibody

Principal name

WT1 / Wilms tumor protein antibody

Alternative names for WT1 / Wilms tumor protein antibody

WT33

SwissProt ID

O62651 (Pig), P19544 (Human)

Gene ID

7490 (WT1), 9589 (WTAP), 5395 (PMS2)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Por (Porcine), Rb (Rabbit), Chk (Chicken), Ze (Zebrafish), African clawed frog, Bov (Bovine), Eq (Equine)

Available hosts

Rabbit, Mouse, Goat

Available applications

Paraffin Sections (P), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunoprecipitation (IP), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Flow Cytometry (F)

Background of WT1 / Wilms tumor protein antibody

WT1 is a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilm\'s tumors. Multiple transcript variants, resulting from alternative splicing at two coding exons, have been well characterized. There is also evidence for the use of non-AUG (CUG) translation initiation site upstream of, and in-frame with the first AUG, leading to additional isoforms.This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilm\'s tumors. Multiple transcript variants, resulting from alternative splicing at two coding exons, have been well characterized. There is also evidence for the use of non-AUG (CUG) translation initiation site upstream of, and in-frame with the first AUG, leading to additional isoforms. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated.

General readings

Rao MK, Pham J, Imam JS, MacLean JA, Murali D, Furuta Y, Sinha-Hikim AP, Wilkinson MF. Tissue-specific RNAi reveals that WT1 expression in nurse cells controls germ cell survival and spermatogenesis. Genes Dev. 2006 Jan 15;20(2):147-52. PMID: 16418481

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

WT1 / Wilms tumor protein (C-term) antibody

Wilm's tumor stained with Anti-WT1 Antibody (Cat.-No AP15857PU) Rabbit Purified Can, Hu, Ms, Por, Rb, Rt IP, P, WB
0.5 ml / €360.00
  Acris Antibodies GmbH

WT1 / Wilms tumor protein antibody

Wilm's tumor stained with Anti-WT1 Antibody (Cat.-No AP15857PU) Rabbit Purified Can, Hu, Ms, Por, Rb, Rt IP, P, WB
1 ml / €440.00
  Acris Antibodies GmbH

WT1 / Wilms tumor protein (C-term) antibody

Wilm's tumor stained with Anti-WT1 Antibody (Cat.-No AP15857PU) Rabbit Purified Can, Hu, Ms, Por, Rb, Rt IP, P, WB
0.1 ml / €180.00
  Acris Antibodies GmbH

WT1 / Wilms tumor protein antibody

Western blot analysis of kidney cell lysate using WT1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

WT1 / Wilms tumor protein antibody

Western Blot- Wilms Tumor 1 Antibody (6F-H2) detects WT1 in 293 cell lysate Mouse IgG1 6F-H2 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

WT1 / Wilms tumor protein antibody

TA302633 (1µg/ml) staining of Human Spleen lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

WT1 / Wilms tumor protein antibody

Wilms Tumor 1 antibody detects Wilms Tumor 1 protein at nucleus and cytosol on mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. Wilms Tumor 1 antibody (TA308577) dilution: 1:500. Rabbit IgG Aff - Purified Hu, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

WT1 / Wilms tumor protein antibody

Immunohistochemistry staining of Paraffin Mesothelioma tissue by WT1 antibody (dilution: 1:100 - 1:500; visualization of staining: Nuclear) Mouse IgG1 6F-H2 Purified Hu P
1 ml / €460.00
  OriGene Technologies, Inc.

WT1 / Wilms tumor protein antibody

WB Suggested Anti-WT1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Hela cell lysate Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

WT1 / Wilms tumor protein antibody

WB Suggested Anti-WT1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: HT1080 cell lysate Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rb, Rt P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

WT1 / Wilms tumor protein antibody

Formalin-Fixed, Paraffin-Embedded Human Wilms tumor stained with WT1 antibody Cat.-No. AM00403SU-L using peroxidase conjugate and DAB chromogen. Note the nuclear staining of tumor cells. Mouse IgG1 6F-H2 Supernatant Hu P
1 ml / €350.00
  Acris Antibodies GmbH

WT1 / Wilms tumor protein (349-439) antibody

Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2a 1E9 Purified Hu E, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

WT1 / Wilms tumor protein (C-term) antibody

AP07246PU-N WT1 Antibody staining of Formalin-Fixed Paraffin-Embedded Human Kidney at 10 µg/ml followed by Biotin conjugated Goat anti-Rabbit IgG secondary antibody, Alkaline Phosphatase-Streptavidin and Chromogen. Rabbit Aff - Purified Hu, Ms, Rt IP, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

WT1 / Wilms tumor protein (Center) antibody

WT1 Antibody (Center E361) (AP54572PU-N)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of WT1 Antibody (Center E361) for immunohistochemistry. Clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu ICC/IF, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

WT1 / Wilms tumor protein antibody

Western Blot analysis of WT1 expression in transfected 293T cell line (H00007490-T02) by WT1 MaxPab polyclonal antibody.

Lane 1: WT1 transfected lysate(34.50 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

WT1 / Wilms tumor protein antibody

WT1 monoclonal antibody (M01), clone 2H4 Western Blot analysis of WT1 expression in A-431 ( Cat # L015V1 ). Mouse IgG2b 2H4 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

WT1 / Wilms tumor protein antibody

WT1 / Wilms tumor protein Mouse IgG2a 3B12 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

WT1 / Wilms tumor protein antibody

WT1 / Wilms tumor protein Mouse IgG2b 1A12 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

WT1 / Wilms tumor protein antibody

WT1 / Wilms tumor protein Mouse IgG 1E2 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

WT1 / Wilms tumor protein antibody

WT1 / Wilms tumor protein Mouse IgG2a 1E9 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

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