WWOX / FOR antibody

Principal name

WWOX / FOR antibody

Alternative names for WWOX / FOR antibody

WOX1, WWOXv1, WWOXv8, WOX2, WWOXv2, WOX3, WWOXdelta6-8, WWOXv4, WWOXdelta5-8, WWOXv3, FOR II, FOR2, FOR I, FOR1, FOR III, FOR3, FOR IV, WW domain-containing oxidoreductase, Fragile site FRA16D oxidoreductase

SwissProt ID

Q91WL8 (Mouse), Q9NZC7 (Human), Q9NZC7-3

Gene ID

51741 (WWOX), 80707 (Wwox)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine)

Available hosts

Rabbit, Mouse

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Dot blot (Dot), Enzyme Immunoassay (E), Frozen Sections (C), Flow Cytometry (F)

Background of WWOX / FOR antibody

WW domain-containing oxidoreductase (Wwox) proteins belong to a family of proteins (Wwox 1–Wwox 8) that are involved in regulation of programmed cell death. Human Wwox gene encodes a putative tumor suppressor WW domain-containing oxidoreductase Wwox1 (also known as Wwox or FOR). Wwox expression has been shown in prostate, lung, breast and other cancers. In addition, numerous aberrant Wwox mRNA transcripts have been found in cancer cells. Wwox gene maps to the common fragile site FRA16D on chromosome 16q23.3-24.1, is altered in breast, esophageal, and ovarian cancer (2). Wwox transcripts with missing Wwox exons are noted in 7 primary tumors (7 of 27; 25.9%) and 5 of 8 cell lines. In addition, loss of heterozygosity at the Wwox locus was observed in 10 primary lung tumors (2).
Wwox1 is a pro-apoptotic protein. In response to stress or apoptotic stimuli, WOX1 became phosphorylated at Tyr33, which enabled its complex formation with activated p53 and JNK1. The p53/Wwox1 complex translocated to the mitochondria and further to the nuclei to mediate apoptosis. Wwox1 mutants, which were inactivated for nuclear translocation or Tyr33 phosphorylation, failed to induce apoptosis, indicating that activation of Wwox1 via Tyr33 phosphorylation, followed by nuclear translocation, is essential for inducing cell death. Wwox1 induced apoptosis synergistically with p53. In contrast, transiently activated JNK1 induced anti-apoptotic response, and this protective activity inhibited Wwox1-induced apoptosis. Taken together, Wwox1 is involved in stress and apoptotic responses, and is likely to regulate the activation of both p53 and JNK1. In murine fetuses, WOX1 was present prevalently in the brainstem, spinal cord and peripheral nerve bundles, but its expression decreased after birth. In parallel, the expression of WOX1, as determined by Western blotting, was significantly reduced in the brain stem and spinal cord of adult mice. Notably, high levels of WOX1 immunoreactivity was observed in the neural crest-derived structures such as cranial and spinal ganglia and cranial mesenchyme during the late fetal stage. In the adult brain, WOX1 is abundant in the epithelial cells of the choroids plexus and ependymal cells, while a low to moderate level of WOX1 is observed within white matter tracts, such as axonal profiles of the corpus callosum, striatum, optic tract, and cerebral peduncle (3). WOX2 is significantly downregulated in the neurons of AD hippocampi (4). A neuronal protectvie hormone, 17-estradiol, also increase the binding of WOX1 and GSK-3 with Tau.

General readings

1. R. L. Friedman, S. P. Manly, M. McMahon, I. M. Kerr, and G. R. Stark, Cell, 38: 745-755, 1984 .Yendamuri S, Kuroki T, Trapasso F, Henry AC,
Dumon KR, Huebner K, Williams NN, Kaiser LR, Croce CM. WW domain containing oxidoreductase gene expression is altered in non-small cell lung
cancer. Cancer Res. 2003 Feb 15;63(4):878-81.
2. Chang NS, Doherty J, Ensign A, Lewis J, Heath J, Schultz L, Chen ST, Oppermann U. Molecular mechanisms underlying WOX1 activation during
apoptotic and stress responses. Biochem Pharmacol. 2003 Oct 15;66(8):1347-54. Related Articles, Links
3. Chang NS, Doherty J, Ensign A. JNK1 physically interacts with WW domain-containing oxidoreductase (WOX1) and inhibits WOX1-mediated apoptosis. J Biol Chem. 2003 Mar 14;278(11):9195-202. Epub 2003 Jan 06.
4. Sze CI, Su M, Pugazhenthi S, Jambal P, Hsu LJ, Heath J, Schultz L, Chang NS. Downregulation of WOX1 induces Tau phosphorylation in vitro: A potential role in Alzheimer's disease. J Biol Chem. 2004 May 7 Epub 2004 May 04.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

WWOX / FOR antibody

  Rabbit Polyclonal WWOX Antibody  
3 ug of anti-WWOX immunoprecipitated the 46 kDa WWOX protein fromt he cytosol of Molt4 T-cells, treated with nocodazole (10uM, 24 hr.) to synchronize mitosis. Rabbit Purified Hu, Ms C, F
0.1 ml / €325.00
  OriGene Technologies, Inc.

WWOX / FOR antibody

  Rabbit anti-WWOX polyclonal antibody  
Western blot analysis of MCF-7 cell lysates with anti-WWOX antibody (1:1000). Rabbit Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

WWOX / FOR antibody

  Rabbit Polyclonal Anti-WWOX Antibody  
WWOX / FOR Rabbit IgG Purified Hu, Ms E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

WWOX / FOR antibody

Western blot (WB) analysis of WWOX antibody (Cat.-No.: AP20494PU-N) in extracts from HepG2 cells. Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

WWOX / FOR (C-term) antibody

WWOX Antibody (C-term) (AP54576PU-N) western blot analysis in mouse testis tissue lysates (35 µg/lane). This demonstrates the WWOX antibody detected the WWOX protein (arrow). Rabbit Ig Aff - Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

WWOX / FOR antibody

Western blot analysis of extracts from HepG2 cells, using WWOX Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, WB
50 µg / €230.00
  Assay Biotech

WWOX / FOR antibody

Western blot analysis of extracts from HepG2 cells, using WWOX Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, WB
0.1 mg / €330.00
  Assay Biotech

WWOX / FOR antibody

WWOX / FOR Rabbit Purified Hu, Ms ICC/IF, IP, P, WB
0.1 ml / €330.00
  Neuromics Antibodies

CRISPR

Catalog No. Species Donor Vector  

WWOX - human gene knockout kit via CRISPR

WWOX - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

WWOX - human gene knockout kit via CRISPR

WWOX - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

WWOX - human gene knockout kit via CRISPR

WWOX - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Wwox - mouse gene knockout kit via CRISPR

Wwox - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Wwox - mouse gene knockout kit via CRISPR

Wwox - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Wwox - mouse gene knockout kit via CRISPR

Wwox - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Wwox - mouse gene knockout kit via CRISPR

Wwox - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

WWOX / FOR (transcript variant 1)

  Recombinant protein of human WW domain containing oxidoreductase (WWOX), transcript variant 1  
WWOX / FOR Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

WWOX / FOR (1-234, His-tag)

WWOX / FOR Human Purified > 85 % by SDS - PAGE E. coli
0.25 mg / €750.00
  Acris Antibodies GmbH

WWOX / FOR (1-234, His-tag)

WWOX / FOR Human Purified > 85 % by SDS - PAGE E. coli
50 µg / €300.00
  Acris Antibodies GmbH

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, WWOX (mGFP-tagged)-Human WW domain containing oxidoreductase (WWOX), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, WWOX (mGFP-tagged)-Human WW domain containing oxidoreductase (WWOX), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, WWOX (mGFP-tagged) - Human WW domain containing oxidoreductase (WWOX), transcript variant 3, 200 uL, >10^7 TU/mL

Lenti ORF particles, WWOX (mGFP-tagged) - Human WW domain containing oxidoreductase (WWOX), transcript variant 3, 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

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