XPO1 / Exportin-1 antibody

Principal name

XPO1 / Exportin-1 antibody

Alternative names for XPO1 / Exportin-1 antibody

Exp1, CRM1, Chromosome region maintenance 1 protein homolog

SwissProt ID

O14980 (Human), Q6P5F9 (Mouse), Q80U96 (Rat)

Gene ID

7514 (XPO1), 3675140 (IPR001792)

Available reactivities

Bov (Bovine), Hu (Human), Ms (Mouse), Rt (Rat), Xen (Xenopus), Chimp (Chimpanzee), Can (Canine), African clawed frog, Eq (Equine), Por (Porcine), Rb (Rabbit), Ye (Yeast), Ze (Zebrafish), GP (Guinea Pig)

Available hosts

Rabbit

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), PLA/Proximity ligation assay (PLA), Frozen Sections (C), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of XPO1 / Exportin-1 antibody

CRM1 mediates the nuclear export of cellular proteins (cargoes) bearing a leucine-rich nuclear export signal (NES) and of RNAs. In the nucleus, in association with RANBP3, CRM1 binds cooperatively to the NES on its target protein and to the GTPase Ran in its active GTP-bound form. Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the cargo from the export receptor. The direction of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. CRM1 also mediates the nuclear export of the Rev protein of HIV-1 and Rex protein of HTLV-1. It is involved in Rex dimerization, U3 snoRNA transport from Cajal bodies to nucleoli. CRM1 is also involved in the control of several cellular processes by controlling the localization of cyclin B, MPAK, and MAPKAP kinase 2. CRM1 is also involved in the regulation NFAT and AP-1. Diffusion of metabolites and small non-nuclear molecules as well as active, mediated import of protein and export of protein and RNA through the nuclear envelope occurs through nuclear pore complexes or NPC's. NPC's contain up to 100 different polypeptides which have a combined mass of about 125 megadaltons. The channel available for passive transport through the NPC is about 9-10 nm in diameter while carrier mediated changes in the NPC result in an approx. 25 nm channel used for larger, actively transported molecules. Active transport of molecules through the NPC is a signal-mediated process that is not well understood. Proteins to be exported from the nucleus to cytoplasm contain leucine-rich nuclear export signals (NESs). NESs are recognized by transport receptors belonging to the importin-beta family of proteins. It has been shown that NES export is mediated by CRM 1 (Chromosome Region Maintenance 1), an importin-beta family member that binds NESs, Ran:GTP, and Ran binding protein (RanBP). CRM 1 mediates the nuclear exportation of numerous proteins, including histone deacetylases (HDACs), viral proteins, immunophilins, and STAT 1.

General readings

Cheong,J.K., (2008) J. Biol. Chem. 283 (17), 11661-11676

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

XPO1 / Exportin-1 antibody

WB Suggested Anti-XPO1 Antibody Titration: 0.2-1 ug/ml; Positive Control: Human heart Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rb, Rt, Ye, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

XPO1 / Exportin-1 antibody

Human Pineal Tissue; Rabbit Anti-XPO1 Antibody . Catalog Number: ARP40465_P050 . Formalin Fixed Paraffin Embedded Tissue: Human Pineal Tissue . Observed Staining: Cytoplasmic in pinealocytes . Primary Antibody Concentration: 1:100 . Other Working Concentrations: 1/600 . Secondary Antibody: Donkey anti-Rabbit-Cy3 . Secondary Antibody Concentration: 1:200 . Magnification: 20X . Exposure Time: 0.5 - 2.0 sec .; Rabbit Anti-XPO1 Antibody (ARP40465_P050) ) in Human Pineal Tissue using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Bov, Can, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

XPO1 / Exportin-1 antibody

WB Suggested Anti-XPO1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: 293T cell lysate; XPO1 is supported by BioGPS gene expression data to be expressed in HEK293T Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

XPO1 / Exportin-1 (390-408) antibody

Spleen: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Bear, Bov, Bt, Can, Chk, Elephant, Eq, Fi, Hst, Hu, Mky, Ms, Op, Prim, Rb, Rt, Xen P, WB
50 µg / €470.00
  Acris Antibodies GmbH

XPO1 / Exportin-1 (C-term) antibody

Western blot analysis of XPO1 antibody (C-term) (AP17839PU-N) in mouse bladder tissue lysates (35ug/lane). XPO1 (arrow) was detected using the purified Pab. Rabbit Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

XPO1 / Exportin-1 antibody

IHC analysis of human spleen. Using XPO1 polyclonal antibody ( Cat # PAB0296 ) at 10 µg/ml. Rabbit Bov, Chimp, Hu C, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

XPO1 / Exportin-1 antibody

Human Heart; Rabbit Anti-XPO1 antibody. Catalog Number: ARP40466 . Paraffin Embedded Tissue: Human Heart cell Cellular Data: cardiac cell of renal tubule. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X; XPO1 antibody - middle region (ARP40466_P050) in Human Heart using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Bov, Can, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

XPO1 / Exportin-1 antibody

Immunohistochemistry: CRM1 Antibody [NB100-40862]-Detection of Human CRM1 (Exportin 1). FFPE section of human breast tumor.  Antibody: Affinity purified rabbit anti-CRM1used at a dilution of 1:250. Rabbit Hu, Ms P
0.1 ml / €500.00
  Novus Biologicals Inc.

XPO1 / Exportin-1 antibody

Immunohistochemistry-Paraffin: CRM1 Antibody [NB100-79802] - FFPE section of human breast tumor.  Antibody: Affinity purified rabbit anti-CRM1 used at a dilution of 1:250.  Detection: DAB staining using Immunohistochemistry Accessory Kit Rabbit IgG Aff - Purified Hu, Ms IP, P, PLA, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

XPO1 / Exportin-1 antibody

Detection of CRM1 (Exportin 1) by Immunoprecipitation.  Samples: Whole cell lysate (1 mg/IP; ¼ of reaction loaded/lane)  from HeLa cells. Antibodies: Affinity purified rabbit anti-CRM1  antibody BL1956 (Cat. No. NB100-79811) used at 3 mcg/mg lysate  for IP. CRM1 was also immunoprecipitated by  rabbit anti-  CRM1 antibodies BL1958 (Cat. No. NB100-79802) and less  efficiently by BL1957, which recognize downstream epitopes on  CRM1. Immunoprecipitated CRM1 was blotted using BL1958 at  0.1 mcg/ml. Detection: Chemiluminescence with an exposure  time of 3 seconds Rabbit IgG Aff - Purified Hu IP
0.1 ml / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

XPO1 / Exportin-1

XPO1 / Exportin-1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

XPO1 / Exportin-1

XPO1 / Exportin-1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

12 Item(s)

per page
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