Dros (Drosophila), Hu (Human), Ms (Mouse), Rt (Rat)
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P), Western blot / Immunoblot (WB), Flow Cytometry (F)
Background of XPO6 / Exportin-6 antibody
Exportins, such as XPO6, recruit cargo in the nucleoplasm in the presence of RAN (MIM 601179)-GTP and form ternary export complexes. These complexes are transported through nuclear pore complexes to the cytoplasm, where GTP is hydrolyzed and the export complex is disassembled.[supplied by OMIM]
Immunohistochemical of paraffin-embedded human ovary using 11408-1-AP(XPO6 antibody) at dilution of 1:25 (under 40x lens)
Western Blot: XPO6/Exportin 6 Antibody [NB100-59853] - Detection of Human XPO6/Exportin 6 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-XPO6/Exportin 6 antibody NB100-59853 used for WB at 1 mcg/ml used for IP at 3 mcg/mg lysate. XPO6/Exportin 6 was also immunoprecipitated by another rabbit anti-XPO6/Exportin 6 antibody, which recognizes an upstream epitope.
Formalin-fixed and paraffin-embedded human brain tissue reacted with XPO6 antibody (C-term) Cat.-No AP17841PU-N, which was peroxidase conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Flow cytometry analysis of Ramos cells using XPO6 antibody (C-term) Cat.-No AP17841PU-N (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.