XRCC5 / Ku80 / Ku86 antibody

Principal name

XRCC5 / Ku80 / Ku86 antibody

Alternative names for XRCC5 / Ku80 / Ku86 antibody

TLAA, G22P2, CTCBF, CTC85, DNA repair protein XRCC5, ATP-dependent DNA helicase II 80 kDa subunit, Lupus Ku autoantigen protein p86, 86 kDa subunit of Ku antigen, Thyroid-lupus autoantigen, CTC box-binding factor 85 kDa subunit, Nuclear factor IV

SwissProt ID

P13010 (Human), P27641 (Mouse)

Gene ID

7520 (XRCC5), 22596 (Xrcc5)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Hst (Hamster), Mky (Monkey), Bov (Bovine), African clawed frog, Can (Canine)

Available hosts

Mouse, Rabbit, Goat

Available applications

Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), Paraffin Sections (P), Immunoprecipitation (IP), Flow Cytometry (F)

Background of XRCC5 / Ku80 / Ku86 antibody

Ku Antigen (DNA-dependent DNA helicase) is a heterodimer (of 72 and 87 kDa polypeptides) which contributes to genomic integrity through its ability to bind DNA double-strand breaks and facilitate repair by the nonhomologous end-joining pathway. A DNA double-strand break is a major lesion that destroys the integrity of the DNA molecule. Such damage is introduced by ionizing radiation. Ku binds to free double-stranded DNA ends and is the DNA-binding component of the DNA-dependent protein kinase. Thus, the Ku protein is involved in DNA repair and in V(D)J recombination, and the Ku-DNA-dependent protein kinase complex may have a role in those same processes. Ku70 and Ku80 share a common topology and form a dyad-symmetrical molecule with a preformed ring that encircles duplex DNA. The binding site can cradle 2 full turns of DNA while encircling only the central 3-4 base pairs. Ku makes no contacts with DNA bases and few with the sugar-phosphate backbone, but it fits sterically to major and minor groove contours so as to position the DNA helix in a defined path through the protein ring. These features are well designed to structurally support broken DNA ends and to bring the DNA helix into phase across the junction during end processing and ligation. Mouse cells deficient for Ku80 display a marked increase in chromosomal aberrations, including breakage, translocations, and aneuploidy. Despite the observed chromosome instabilities, Ku80 -/- mice have only a slightly earlier onset of cancer. Loss of p53 synergizes with Ku80 to promote tumorigenesis such that all Ku80 -/- and p53 -/- mice succumb to disseminated pro-B-cell lymphoma before 3 months of age. The p70/p80 complex binds to the ends of double-stranded DNA in a cell cycle-dependent manner, being associated with chromosomes of interphase cells, followed by complete dissociation from the condensing chromosomes in early prophase. Some patients with systemic lupus erythematosus produce very large amounts of autoantibodies to p70 and p80. The autoantibody has been found in patients with autoimmune thyroid disease (Graves disease) as well as in those with lupus.

General readings

General / background references:Jackson SP The recognition of DNA damage. Curr Opin Genet Dev 6:19-25 (1996). PubMed PMID: 8791484
Weaver DT What to do at an end: DNA double-strand-break repair. Trends Genet 11:388-92 (1995). PubMed PMID: 7482764

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Ku70/80 (XRCC6/5) (C-term) antibody

Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Ku70/80 (XRCC6/5) (C-term) antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Ku70/80 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, ICC/IF, P, WB
50 µg / €205.00
  Assay Biotech

Ku70/80 (XRCC6/5) antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Ku70/80 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, ICC/IF, P, WB
0.1 mg / €310.00
  Assay Biotech

XRCC5 / Ku80 / Ku86 antibody

Immunohistochemistry (IHC) analysis of Ku80 Antibody (Cat.-No AP06398PU-N) in paraffin-embedded human lung carcinoma tissue. Rabbit Aff - Purified Hu ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

XRCC5 / Ku80 / Ku86 antibody

Ku80 antibody [N3C2], Internal detects XRCC5 protein by Western blot analysis. A. 30 ug 293T whole cell lysate/extract. B. 30 ug A431 whole cell lysate/extract. C. 30 ug HeLa whole cell lysate/extract. D. 30 ug HepG2 whole cell lysate/extract. 7.5 % SDS-P Rabbit IgG Aff - Purified Hu ICC/IF, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

XRCC5 / Ku80 / Ku86 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-XRCC5 rabbit polyclonal antibody. (TA590469) Rabbit IgG Purified Can, Hu, Mky, Ms E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

XRCC5 / Ku80 / Ku86 antibody

Immunohistochemical analysis of paraffin-embedded human tonsil tissues (left) and human colon cancer tissues (right) using XRCC5 mouse mAb with DAB staining. Mouse IgG1 5C5 Ascites Hu, Ms E, ICC/IF, P, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

XRCC5 / Ku80 / Ku86 antibody

Human Pancreas; XRCC5 antibody - C-terminal region (ARP34121_T100) in Human Pancreas cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Bov, Hu, Ms, Rt P, WB
0.1 mg / €310.00
  AVIVA Systems Biology

XRCC5 / Ku80 / Ku86 antibody

Human tonsil stained with anti-Ku80 antibody Rabbit IgG SP130 Purified Bov, Hu, Rt P, WB
0.5 ml / €510.00
  Acris Antibodies GmbH

XRCC5 / Ku80 / Ku86 antibody

Human tonsil stained with anti-Ku80 antibody Rabbit IgG SP130 Purified Bov, Hu, Rt P, WB
1 ml / €730.00
  Acris Antibodies GmbH

XRCC5 / Ku80 / Ku86 antibody

Human tonsil stained with anti-Ku80 antibody Rabbit IgG SP130 Purified Bov, Hu, Rt P, WB
0.1 ml / €290.00
  Acris Antibodies GmbH

XRCC5 / Ku80 / Ku86 antibody

TA303099 (0.1µg/ml) staining of HeLa cell lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
20 µg / €200.00
  Acris Antibodies GmbH

XRCC5 / Ku80 / Ku86 (C-term) antibody

Human placenta stained with anti-Ku80 antibody Rabbit IgG Purified Hu P
0.5 ml / €360.00
  Acris Antibodies GmbH

XRCC5 / Ku80 / Ku86 (C-term) antibody

Human placenta stained with anti-Ku80 antibody Rabbit IgG Purified Hu P
1 ml / €520.00
  Acris Antibodies GmbH

XRCC5 / Ku80 / Ku86 (C-term) antibody

Human placenta stained with anti-Ku80 antibody Rabbit IgG Purified Hu P
0.1 ml / €190.00
  Acris Antibodies GmbH

XRCC5 / Ku80 / Ku86 antibody

Human placenta stained with anti-Ku80 antibody Rabbit IgG Purified Hu P
0.5 ml / €360.00
  Acris Antibodies GmbH

XRCC5 / Ku80 / Ku86 antibody

Human placenta stained with anti-Ku80 antibody Rabbit IgG Purified Hu P
1 ml / €520.00
  Acris Antibodies GmbH

XRCC5 / Ku80 / Ku86 antibody

Human placenta stained with anti-Ku80 antibody Rabbit IgG Purified Hu P
0.1 ml / €190.00
  Acris Antibodies GmbH

XRCC5 / Ku80 / Ku86 antibody

TA303099 (0.1µg/ml) staining of HeLa cell lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

XRCC5 / Ku80 / Ku86 antibody

XRCC5 Antibody (CenterK439) (Cat. #TA324453) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the XRCC5 antibody detected the XRCC5 protein (arrow). Rabbit IgG Purified Hu F, ICC/IF, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

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